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Identifikasi bakteri gram negatif batang pada urine suspek infeksi saluran kemih mahasiswi di Poltekkes Kemenkes Aceh Erlinawati Erlinawati; Asri Jumadewi; Fitriana Fitriana
Jurnal SAGO Gizi dan Kesehatan Vol 5, No 2 (2024): April
Publisher : Poltekkes Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v5i2.1547

Abstract

Background: Urinary tract infection is a disease caused by the growth of microorganisms in the human urinary tract.  25-35% of adult women experience UTIs based on data from clinical epidemiology studies. On this campus, students are predominantly female with busy activities, so they tend to consume less drink according to the standard and often experience symptoms of UTI caused by gram-negative rod bacteria.Objectives: to determine the presence or absence and type of gram-negative rod bacteria in the urine of female students suspected of UTI in the D-III TLM Study Program.Methods: analytical observational design with cross sectional design, which was conducted at UPTD Labkes Center and Medical Device Testing in August 2023 with a sample of 20 female students' urine.  Data analysis in this study is univariate analysis to find the number and type of gram-negative rod bacteria and observe bacterial growth.Results: the identification results showed that the number of positive samples was 75% and the negative samples were 25%.  The types of bacteria are: Escherichia coli (35%), Enterobacter Sp (20%), Citrobacter disversus (5%) and Serratia rubidae (15%).  This is because most D-III TLM students have high activity, so they pay less attention to water intake or rarely drink and they also often experience anyang or difficulty urinating which is a symptom of UTI.Conclusion: In the urine of female students suspected of UTI in the D-III TLM Study Program of the Poltekkes Kemenkes Aceh there are gram-negative rod bacteria.  
Indonesia PENGARUH PEMANASAN MEDIA NA Erlinawati Sukedi; Fitriana Fitriana; Asri Jumadewi
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikalsains.v13i1.6389

Abstract

The Microbiology Laboratory at the Department of Medical Laboratory Technology, Poltekkes Kemenkes Aceh, often uses Nutrient Agar (NA) media to count the total number of bacteria in various bacteriology practicums. This media, after being made and cooled to harden, is stored in a refrigerator at 2-8°C for 1-2 weeks. Before use, the media must be reheated at 100°C to thaw it. This process is often done repeatedly to save labor and facilities in the laboratory. However, repeated heating of NA media is thought to lower the pH of the media and reduce the number of bacterial colonies that grow, potentially affecting the accuracy of microbiological test results. Therefore, this study was conducted to determine the extent to which repeated heating of NA media affects the pH and growth of bacterial colonies. Experimental method with a completely randomized design. Data were collected through repeating the heating of NA media 4 times, and the pH and number of colonies growing were measured after certain time intervals until heating was done 5 times. The results showed that repeated heating of NA media affected pH and bacterial growth. The more often the media is heated, the lower the pH of the media, and the fewer bacterial colonies grow. Repeated heating of Nutrient Agar (NA) media has an effect on bacterial TPC or the amount of Escherichia coli bacterial growth.
Penyuluhan Bahaya Kandidiasis Sebagai Penyakit Menular Seksual Pada Mahasiswa Tingkat Akhir Poltekkes Kemenkes Aceh Rahmayanti Rahmayanti; Erlinawati Erlinawati; Siti Hadijah; Darmawati Darmawati; Fitriana Fitriana
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 6, No 2 (2026): Abdira
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v6i2.1730

Abstract

Sexually transmitted infections have a significant impact on sexual and reproductive health worldwide. Yeast infections are not generally considered sexually transmitted diseases (STDs) because they are caused by an overgrowth of fungi already present in the body. However, sexual activity can sometimes cause this imbalance. The purpose of this community service is to increase the knowledge and understanding of candidiasis among the public, particularly final-year students of the Aceh Ministry of Health Polytechnic of Health. The method used was an outreach method, using visual aids and a questionnaire as an instrument. The target audience was 80 final-year students majoring in TLM at the Aceh Ministry of Health Polytechnic of Health. The activity began with a pre-test followed by a presentation of the activity material. After the presentation, a question-and-answer session was held with participants given the opportunity to ask questions, followed by brochures being distributed, followed by a post-test. Data analysis showed a 95% increase in student knowledge after the outreach. It can be concluded that this outreach activity ran smoothly and successfully, with participants' knowledge increasing after the outreach.