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All Journal MEDICA (International Medical Scientific Journal)
Etiek Nurhayati
Department of Medical Laboratory Technology, Politeknik Kesehatan Kementerian Kesehatan Pontianak, Pontianak, West Kalimantan, Indonesia
Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Exploring C-Reactive Protein Levels in Menstruating Female Students: A Descriptive Study Agiesa Khoirunzia; Herlinda Djohan; Linda Triana; Etiek Nurhayati
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.482

Abstract

During menstruation, prostaglandins induce uterine contractions, which contribute to the thickening of the endometrial mucosa and the constriction of blood vessels. Elevated levels of prostaglandins may influence C-Reactive Protein (CRP) levels, a marker that increases in response to inflammation or infection as part of the body's immune response. This study aimed to examine CRP levels in female students during menstruation. A descriptive research design was employed to assess CRP levels in menstruating female students. The sample consisted of blood serum collected from 30 students at Madrasah Aliyah Pondok Pesantren Pembangunan Ushuluddin in Singkawang City. The results showed that among the participants, 5 students aged 16 and 1 student aged 15 exhibited positive CRP levels. Regarding the duration of menstruation, positive CRP levels were detected in 4 students on the first day, 1 student on the second day, and 1 student on the third day. Overall, 20% of the students showed positive CRP levels, while 80% had negative CRP levels. The conclusion indicates that a subset of menstruating female students exhibited elevated CRP levels, particularly during the early days of menstruation. This suggests that menstruation, likely through prostaglandin-induced inflammation, may be associated with an increase in CRP levels in some individuals.
Utilization of Red Dragon Fruit (Hylocereus polyrhizus) as an Alternative Dye for Threadworm Eggs (Ascaris lumbricoides) Desti Erfiana; Etiek Nurhayati; Gervacia Jenny Ratnawaty
MEDICA (International Medical Scientific Journal) Vol. 7 No. 1 (2025): MEDICA (International Medical Scientific Journal)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/medica.v7i1.483

Abstract

The red dragon fruit (Hylocereus polyrhizus) contains a pigment called anthocyanin, which gives it its red color and has the potential to be used as a natural dye. In laboratory microscopic preparations, eosin is used as a standard dye. Natural dyes have the advantage of not causing negative effects like cancer or environmental damage. This study aimed to examine the potential of red dragon fruit (Hylocereus polyrhizus) as an alternative dye for the examination of Ascaris lumbricoides eggs. The study design used was static group comparison, with local red dragon fruit samples from Pontianak Tenggara District. Laboratory examination methods involved direct microscopic observation using a 2% eosin control. In this study, the juice from both the skin and flesh of the red dragon fruit was extracted and prepared in solutions of 80%, 90%, and 100% concentrations with four replications. The results showed that the skin of the red dragon fruit at concentrations of 80%, 90%, and 100% was not suitable for coloring the microscopic preparation for observing worm eggs. However, the juice from the flesh of the red dragon fruit at concentrations of 80%, 90%, and 100% could color the microscopic preparations, and the Ascaris lumbricoides eggs could be identified. At the 80% concentration of red dragon fruit flesh, the average score was 2, indicating low contrast background, weak color absorption by the eggs, and unclear egg details. At 90% and 100% concentrations, the average score was 3, indicating a clear contrast background, good color absorption by the eggs, and clear egg detail, similar to eosin 2% staining. This study concludes that the skin of red dragon fruit cannot be used for staining microscopic preparations, while the flesh of red dragon fruit can be used for staining Ascaris lumbricoides eggs, with optimal concentrations of 90% and 100%.
Co-Authors Agiesa Khoirunzia Desti Erfiana Herlinda Djohan Linda Triana Ratnawaty, Gervacia Jenny