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All Journal Jurnal Biosains Pascasarjana Humantech : Jurnal Ilmiah Multidisiplin Indonesia
Sitanggang, Robestri Natalia
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Public Health

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COMPARISON OF 10% BUFFERED FORMALIN NEUTRAL FIXATION SOLUTION WITH BOUIN ON MICROSCOPIC IMAGES OF CHICKEN HEPAR AND CEREBRUM WITH HEMATOXYLIN EOSIN (HE) STAINING Sitanggang, Robestri Natalia
Jurnal Biosains Pascasarjana Vol. 27 No. 1 (2025): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v27i1.2025.5-9

Abstract

The fixation process is an important stage in the manufacture of histopathology preparations. Fixation aims to prevent autolysis and tissue degradation, so that the results can be observed both anatomically and microscopically. Fixation solutions that are often used in routine histopathological examinations are 10% Formalin Buffer Neutral and Bouin. This study was used to determine the results of the comparison of 10% NBF fixation solution with Bouin on the microscopic picture of hepar and cerebrum with hematoxylin eosin staining. The type of research used is a cross sectional approach with a qualitative descriptive design. Sampling was performed on chicken animals. The samples used were hepar and cerebrum fixed with 10% NBF and 10% Bouin for 12 hours, stained with Hematoxylin Eosin and observed microscopically. Then give an assessment with good, less good, or not good categories based on assessment indicators based on cell color and shape. The results showed a microscopic picture of hepatic and cerebrum tissue as much as 6 preparations fixed with 10% NBF liquid showed good results. While hepatic and cerebrum tissue fixed with bouin liquid as many as 6 preparations showed poor results. The conclusion is that there are differences in the microscopic results of hepatic and cerebrum tissue fixed with 10% NBF solution and Bouin. Good microscopic observation results based on the assessment indicator criteria are tissues fixed with 10% NBF liquid.
The Impact of Pro-Inflammatory Cytokines on the Clinical Manifestations and Disease Progression of Spondyloarthritis: a Narrative Review Sari, Lia Cahya; Sitanggang, Robestri Natalia; Qudsiyyah, Farida
Humantech : Jurnal Ilmiah Multidisiplin Indonesia Vol. 4 No. 2 (2025): Humantech : Jurnal Ilmiah Multidisiplin Indonesia
Publisher : Program Studi Akuntansi IKOPIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32670/qn0cnp03

Abstract

Spondyloarthritis (SpA) is a group of inflammatory joint and spine diseases with various clinical manifestations. The classification and diagnosis of SpA are determined based on observations of the sacroiliac joints, hip joints, enthesitis, or the presence of human leukocyte antigen B27 (HLA-B*27). The main clinical manifestations of SpA are inflammatory pain, stiffness, and swelling of the joints and spine. Although the pathogenesis of SpA is not fully understood, genetic and environmental factors can lead to inflammation by producing pro-inflammatory cytokines such as TNF-α and the IL-23/IL-17 axis, which cause dysregulation of immune cells and stromal cells. This review aims to find out the impact of pro-inflammatory cytokines on the clinical manifestations and disease progression of Spondyloarthritis. Pro-inflammatory cytokines, such as TNF-α, IL-17, and IL-23 have an important role in the pathogenesis and progression of Spondyloarthritis. IL-17A stimulates the expression of RANKL and inhibits Wnt signaling, thereby suppressing osteoblast activity, promoting osteoclast differentiation and bone resorption in axSpA and PsA. IL-17 also trigger arthritis, spondylitis, and conjunctivitis in ReA. Increased expression of IL-17, IL-23, and TNF-α due to microbiota activity provides immune cell recruitment and mucosal injury, thus maintaining chronic inflammation in IBD. Pro-inflammatory cytokines have an impact on clinical manifestations of spondyloarthritis by stimulating an inflammatory response in the joints, leading to pain, and influencing disease progression by causing bone and joint tissue destruction. They also contribute to the formation of scar tissue around the joints, reducing mobility and causing significant stiffness in the spine
Co-Authors Qudsiyyah, Farida Sari, Lia Cahya