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All Journal JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Journal of Research Applications in Community Services
Kusumaningtyas, Ida Ayu Mas Amelia
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Optimization of RNA Extraction Method of Banana Leaf Using Li Buffer and GENEzol Reagent Kusumaningtyas, Ida Ayu Mas Amelia; Pambudi, Arief; Effendi, Yunus
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 10, No 2 (2025): Mei 2025
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v10i2.2092

Abstract

High quality extracted RNA from plant tissues is used in downstream transcriptomic application that needs high quantity for multiple analysis. Attaining high quality and high yield for banana plant tissue is a challenge with its high number of secondary metabolites becoming contaminants, effecting the purity of RNA extracted. A procedure to extract banana plant RNA with high quality and quantity in this study is developed to be simple, robust, affordable and accessible using basic lab equipment and materials. This study optimizes RNA extraction from the leaf of juvenile Pisang Susu (Musa spp.) using a combination of Li buffer (modified CTAB) and GENEzol reagent. Treatment differs in the use of β-mercaptoethanol and the duration of sample precipitation with isopropanol. NucleoSpin RNA Plant extraction kit was made as comparison. Measurement of RNA quantity used quantus fluorometer, and quality measured by spectrophotometer. Results showed that the addition of β-mercaptoethanol in Li buffer is vital with samples left to precipitate overnight providing best results. The total RNA obtained had a higher yield compared to the commercial kit with 108 – 211 ng/µl and 0.35 – 0.37 ng/µl respectively. Light absorbance A260/280 indicating sample purity between method has similar RNA quality of 0.917 – 1.084 and 0.843 – 1.026. This study proved that modification of CTAB buffer using Li buffer for RNA extraction resulted in better quality and quantity of RNA compared to the commercial RNA extraction kit.Keywords – Banana, Optimization, RNA Extraction.
Sosialisasi Hidup Ramah Lingkungan dan Pelatihan Menanam dengan Metode Hidroponik Sederhana Pada Anak PAUD Desa Candali Bogor Evandra, Azka Salma; Mudrikah, Isna Ibnah; Anugrah, Dinda Bunga; Kusumaningtyas, Ida Ayu Mas Amelia; Diandra, Azzahra Putri; Dewirani, Randilla; Fattah, Iqbal; Santoso, Bagus Dwi; Nurhasanah, Nunung
Journal of Research Applications in Community Service Vol. 1 No. 2 (2022): Journal of Research Applications in Community Service
Publisher : Universitas Nahdlatul Ulama Sunan Giri Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32665/jarcoms.v1i2.1303

Abstract

Desa Candali adalah salah satu desa binaan Lembaga Amil Zakat (LAZ) Al-Azhar. Desa ini memiliki banyak potensi dan kelebihan, namun terdapat permasalahan yang dimiliki, salah satunya adalah keunggulan sumber daya alam yang dimiliki tidak diikuti dengan keunggulan sumber daya manusia dalam hal pertanian yang berkelanjutan. Penanganan solusi yang diangkat adalah melakukan sosialisasi kepada generasi selanjutnya mengenai pentingnya menjaga lingkungan sekitar serta slot deposit 5000 pelatihan menanam dengan metode hidroponik kepada peserta PAUD Desa Candali. Pemberian pengetahuan pada anak-anak diharapkan dapat melekat dan dipraktekkan secara terus-menerus. Anak-anak PAUD sangat antusias dalam mengikuti kegiatan sehingga kegiatan dapat berjalan dengan lancar. Hasil dari kegiatan yang dilakukan memberikan peningkatan pengetahuan anak-anak PAUD mengenai bagaimana cara menanam dengan metode hidroponik sederhana serta bagaimana cara menjaga lingkungan agar tetap terjaga dan terhindar dari kerusakan. Hasil persentase keberhasilan untuk sosialisasi hidup ramah lingkungan sebesar 100%, sedangkan pelatihan menanam hidroponik sebesar 90%. Hal ini menunjukkan perlu adanya perbaikan kegiatan pelatihan menanam hidroponik dengan menambahkan jangka waktu pelatihan agar pemahaman dan penerapan kegiatan ini tersampaikan dengan lebih baik.
Co-Authors Anugrah, Dinda Bunga Arief Pambudi Dewirani, Randilla Diandra, Azzahra Putri Evandra, Azka Salma Fattah, Iqbal Mudrikah, Isna Ibnah Nunung Nurhasanah Santoso, Bagus Dwi Yunus Effendi