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All Journal Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Majalah Kedokteran Andalas Indonesian Journal of Biomedicine and Clinical Sciences
Fajar Liana, Delima
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Biofilm detection of clinical isolates of Klebsiella pneumoniae from Pontianak, West Kalimantan Anggie, Joycely; Fajar Liana, Delima; Mardhia, Mardhia; Mahyarudin, Mahyarudin; Agustina, Risa
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 2 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/10.22146/inajbcs.v57i2.18918

Abstract

Klebsiella pneumoniae (K. pneumoniae) belongs to the Enterobacteriaceae family, which is known as Gram-negative, encapsulated, and non-motile bacteria. One of its most important virulence factors of K. pneumoniae is the ability to form biofilms. A high percentage of K. pneumoniae as biofilm formation is associated with a high incidence of antibiotic resistance, leading to higher morbidity and mortality. This study aimed to evaluate biofilm formation of clinical isolates of K. pneumonia from Pontianak, West Kalimantan. A total of 24 K. pneumoniae clinical isolates from various specimens were subjected to biofilm formation detection. The biofilm formation was detected by crystal violet formation at wavelength of 570 nm as indicator using microtiter plate assay. As control of biofilm formation was Staphylococcus aureus ATCC 25923, and as control of non-biofilm formation was Staphylococcus epidermidis ATCC 12228. Data on biofilm formation of the isolates from various specimens were analyzed using Fisher’s exact test with SPSS Ver. 26. All the K. pneumoniae clinical isolates were biofilm producer consisting of 19 isolates were strong biofilm producer, four were moderate biofilm producer, and one isolates was weak biofilm producer. No association between biofilm formation and specimen type was observed (p=0.541). In conclusion, all K. pneumoniae clinical isolates from Pontianak, West Kalimantan are identified as biofilm producer.
mrkA, mrkD, AND fimH BIOFILM GENES DETECTION: A STUDY ON Klebsiella pneumoniae CLINICAL ISOLATES ‘Ariqah ‘Azizah, Intan; Mardhia, Mardhia; Fajar Liana, Delima; Mahyarudin, Mahyarudin
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 19 No 1 (2025): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan (In Press)
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v19i1.10331

Abstract

Biofilm formation is a virulence factor contributing to the pathogenicity and antibiotic resistance of Klebsiella pneumoniae. This process occurs due to biofilm genes in clinical isolates, especially the mrkA, mrkD, and fimH genes. These three genes are related the quality of biofilms formed, which lead to chronic and prolonged infections. This study was conducted to determine the presence of mrkA, mrkD, and fimH genes as biofilm formers in Klebsiella pneumoniaeclinical isolates. The study used a descriptive in vitro laboratory study, with total of 24 Klebsiella pneumoniae clinical isolates from the collection of the Department of Microbiology, Faculty of Medicine, Universitas Tanjungpura. Isolates were subculture in MacConkey Agar, Gram staining, followed by DNA extraction and gene detection using PCR. The results showed the presence of the mrkA gene in 91.6% of the isolates, while the mrkD and fimH genes were each in 87.5% of the isolates. The high percentage of biofilm gene detected, should increase health workers and stakeholder awarness about the impact.
Potensi antibakteri bawang putih dan bawang hitam siung tunggal (allium sativum l.) terhadap proteus mirabilis Rahmat Dani, Kafa; Fajar Liana, Delima; Mahyarudin, Mahyarudin
Majalah Kedokteran Andalas Vol. 48 No. 4 (2025): MKA October 2025
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v48.i4.p426-435.2025

Abstract

Latar Belakang: Resistensi Proteus mirabilis meningkat; eksplorasi antibakteri bawang hitam dan bawang putih siung tunggal menjadi alternatif. Tujuan: Menilai serta membandingkan potensi antibakteri ekstrak etanol herbal bawang hitam siung tunggal serta herbal bawang putih siung tunggal terhadap Proteus mirabilis. Metode: Merupakan penelitian true experiment dengan melakukan observasi setelah perlakuan diberikan. Uji antibakteri menggunakan metode difusi cakram. Uji statistik memakai rumus uji Shapiro Wilk, uji alternatif menggunakan rumus Kruskal Wallis serta rumus uji Mann Whitney sebagai penutup. Hasil: Percobaan memperlihatkan ekstrak etanol bawang putih siung tunggal tidak memiliki aktivitas antibakteri apapun pada semua konsentrasi uji. Sementara itu, ekstrak etanol tanaman herbal bawang hitam siung tunggal dengan kepekatan 75% serta 100% menunjukkan adanya zona jernih (zona antibakteri) dengan ukuran masing-masing 8,2 milimeter dan 8,4 milimeter. Simpulan: Bawang hitam siung tunggal memiliki potensi dalam mencegah pertumbuhan bakteri Proteus mirabilis, dengan konsentrasi minimum efektif sebesar 75%.
Co-Authors Agustina, Risa Anggie, Joycely Mahyarudin, Mahyarudin Mardhia, Mardhia Rahmat Dani, Kafa ‘Ariqah ‘Azizah, Intan