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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Putri, Hilda Arsyah Eka
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Isolation and Characterization of Pathogenic Microbes Origin in Strawberry (Fragaria sp.) Based on Koch's Postulates Asharo, Rizal Koen; Indrayanti, Reni; Damayanti, Aldira Putri; Putri, Hilda Arsyah Eka; Nabilah, Saskia; Pasaribu, Pinta Omas
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 2 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i2.269

Abstract

Strawberry (Fragaria sp.) is a horticultural plant originating from the Americas. Strawberries have a distinctive sweet and refreshing taste, and contain lots of vitamins and antioxidants that are useful for body health. But strawberries are also easily damaged. This damage can be caused by spoilage microorganisms such as bacteria and fungi that can grow if conditions permit such as the presence of appropriate temperature and humidity conditions. In this experiment, aims to isolate pathogenic microorganisms that cause disease in symptomatic strawberries and prove that these pathogens actually cause disease using Koch's Postulates method and then descriptive analysis is carried out. From the experimental results, it was found that soft rot disease in strawberries was characterized by rotten fruit parts that looked wet, slightly brownish in color, slimy, there were fine white threads and emitted a foul odor with a percentage of IP (disease occurrence) of 100% and KP (disease severity) of 92.66% where based on the score the damage was the highest.
Isolation and Characterization of Pathogenic Mold Causing Potato Tuber Rot Disease Asharo, Rizal Koen; Indrayanti, Reni; Amala, Azizatul; Raihan, Eldrian Daffa; Tampanguma, Raymond Rayhand; Putri, Hilda Arsyah Eka; Pasaribu, Pinta Omas; Wardana, Nurul Assyifa
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.6355

Abstract

The potato plant (Solanum tuberosum L.) is a perennial crop that contains high amounts of carbohydrates, minerals, and vitamins in its tubers, making it a carbohydrate-rich alternative food to rice or corn. One of the diseases that often appear on potato plants is potato rot caused by pathogenic molds. This study aims to isolate and characterize pathogenic fungi that cause blight on potato tubers based on Koch's Postulates. Potato pathogenic molds were isolated from potato tubers that had been rotted, then the molds were grown on PDA and purified twice. The purified isolates were then inoculated onto 30 healthy potato tubers and incubated for 7 days. Healthy potato tubers experienced the same symptoms as potato tuber rot. The isolated pathogenic fungi were then characterized so that the pathogenic fungi of Phytophthora infestans were obtained. In this study, Koch's Postulate method was successfully applied to isolate and characterize the pathogenic fungi that cause potato tuber rot. The pathogenic fungi caused a potato tuber rot disease incidence value of 100% with a disease severity value of 60.7%. The two factors that determine the disease incidence and disease severity values are internal factors (genes and traits of the pathogen) and external factors (environment).
Co-Authors Amala, Azizatul Damayanti, Aldira Putri Nabilah, Saskia Pinta Omas Pasaribu Raihan, Eldrian Daffa Reni Indrayanti Rizal Koen Asharo Tampanguma, Raymond Rayhand Wardana, Nurul Assyifa