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AKTIVITAS Stenotrophomonas rhizophila DAN Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Ganoderma boninense Rupaedah, Bedah; Amanda, Debby Viola; Indrayanti, Reni; Asiani, Nia; Sukmadi, Bambang; Ali, Asep; Wahid, Abdul; Firmansyah, Taufik; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninenseÂ ABSTRACTBasal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp.Â ABSTRAKPenyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila, Â Â Â Trichoderma sp.

AKTIVITAS Stenotrophomonas rhizophila DAN Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Ganoderma boninense Rupaedah, Bedah; Amanda, Debby Viola; Indrayanti, Reni; Asiani, Nia; Sukmadi, Bambang; Ali, Asep; Wahid, Abdul; Firmansyah, Taufik; Sugianto, Mahmud
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninenseÃ‚Â ABSTRACTBasal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp.Ã‚Â ABSTRAKPenyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila, Ã‚Â Ã‚Â Ã‚Â Trichoderma sp.

The Influence of Self-Efficacy and Learning Independence Againts The Outcomes of The Study Material on Ecosystem Biology High School Student of Grade X Ervan Nurkholis; Mieke Miarsyah; Reni Indrayanti
Indonesian Journal of Science and Education (IJOSE) Vol 2, No 1 (2018): Indonesian Journal of Science and Education
Publisher : Fakultas Keguruan dan Ilmu Pendidikan

In education word one of a serious problem is a high to low student learning outcomes. Good learning outcomes will be achieved when students have the self-efficacy and learning independence in overcoming learning difficulties which affect them. This research aims to find out and analyze the influence of self-efficacy and learning independence against the outcomes of the study of biology students. The research method used is ex pose facto 2x2 factorial design. The sample of this research is the grade X with number of 74 students. The instruments in this research are self-efficacy questioner, learning independence questioner and the outcomes study quesioner. Prerequisites testing conducted distributed normal data using Kolmogorov Smirnov and homogenous test using Bartlet test. Based on hypothesis testing, it was concluded that there is no influence of self-efficacy and learning independence against the outcomes of the study and there is influence between learning independence on student learning outcomes at Senior High School in Bekasi.Keywords: self-efficacy, learning independence, student learning outcomes, ex post facto

Corelation Between Learning Motivation and Learning Outcomes of Senior High School Students in Heredity Material Rahayu Rahayu; Mieke Miarsyah; Reni Indrayanti
Indonesian Journal of Science and Education (IJOSE) Vol 2, No 1 (2018): Indonesian Journal of Science and Education
Publisher : Fakultas Keguruan dan Ilmu Pendidikan

Learning outcomes is an ability that must be owned by learner after getting a learning experience. This ability is not an independent thing, but has correlation with many factors, such as learning motivation. The aim of this research is to know the correlationn between learning motivation and learning outcomes of senior high school students in Heredity materials. This research was conducted on students grade XII of SMA IT Thariq Bin Ziyad on first and second semester in the school year 2016/2017. Research method that is used is survey through correlational study. Data were collected by using questionnary for learning motivation, and test instrument for learning outcomes in Heredity material. All data were distribute normally and homogenuous. Data were analysed by simple linear regression test showed that regression equation y = 9,72 + 0,34x. Result of this research showed that there is possitive correlation between learning motivation and learning outcomes of students in Heredity materials with correlation coofisient 0,414. This result illustrate that higher learning motivation will make students’ learning outcomes in Heredity material become better. The other way, if students learning motivation is low, the learning outcomes will be low as well.Keywords: Learning motivation, learning ourcomes, Heredity material

Evaluasi Keragaman Fenotipik Pisang Cv. Ampyang Hasil Iradiasi Sinar Gamma di Rumah Kaca Reni Indrayanti; Nurhajati A. Mattjik; Asep Setiawan; , Sudarsono
Jurnal Hortikultura Indonesia Vol. 3 No. 1 (2012): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTBanana and plantain are important for food security. Increase of genetic variability is difficult because most of edible bananas are triploid, sterile and parthenocarpy. Therefore, breeding through convetional method are difficult. The objective of this research were to evaluate phenotypic variation of banana cv. Ampyang (Musa acuminata, AAA, subgroup non-Cavendish) regenerated from in vitro induced mutation by gamma irradiation in a greenhouse. The objectives of this research were to characterize and evaluate phenotypic diversity of banana cv. Ampyang plant, in vi tro regenerated from gamma irradiated plantlet in a greenhouse. The phenotypes (both quantitative and qualitative characters) were evaluated when the plants were at six months after acclimatization. Result indicated banana plant sregenerated from gamma irradiated explants exhibited lower plant height, shorter leaf, and leaf length by leaf width ratio than from non-irradiated ones. Population of banana cv. Ampyang gamma irradiated at30, 40, 45 and 50 Gy showed broader variation in qualitative characters than those treated with 0 Gy. Banana cv. Ampyang originated from explants treated with 0 Gy showed similar stomatal density than those from explants treated with 45 and 50 Gy. On the other hand, those originated from explants treated with 20, 25, 30 or 40 Gy showed lower stomatal densities than those treated with 0 Gy. The results have demonstrated that mutation breeding with in vitro technique could produce morphological changes as well as increase in variability of quantitative traits. A number of these parameters were supposed to be applicable for characterization of variation in other banana cultivars.Key words: Musa acuminata (AAA), stomata density, doses of gamma irradiationABSTRAKPisang penting untuk keamanan pangan. Peningkatan keragaman genetik pada pisang sulit dilakukan karena sebagian besar pisang yang dapat dimakan bersifat triploid, steril dan partenokarpi.Oleh sebab itu, pemuliaan tanaman secara konvensional sulit dilakukan. Tujuan penelitian ini ialah untuk mengevaluasi variasi fenotipik pisang cv. Ampyang (Musa acuminata, AAA, subgroup non-Cavendish)yang diregenerasikan dari kultur in vitro dan diinduksi mutasi dengan sinar gamma. Fenotipe (karakter kualitatif dan kuantitatif) dievaluasi ketika tanaman berumur 6 bulan setelah aklimatisasi. Hasil penelitian menunjukkan bahwa tanaman pisang yang diregenerasikan dari eksplan yang diiradiasi sinar gamma memiliki tinggi tanaman yang lebih rendah, daun yang lebih pendek, dan rasio panjang dan lebar daun yang lebih rendah dibanding tanaman non-iradiasi. Populasi pisang cv. Ampyang hasil iradiasi sinar gamma 30, 40, 45 dan 50 Gy menunjukkan variasi yang lebih lebar dalam karakter kualitatif dibanding tanaman kontrol (0 Gy). Pisang cv. Ampyang asal eksplan kontrol (0 Gy) menunjukkan densitas stomata yang sama dengan tanaman eksplan yang diiradiasi sinar gamma 45 dan 50 Gy. Tanaman yang berasal dari eksplan yang diiradiasi sinar gamma 20, 25, 30 or 40 Gy menunjukkan densitas stomata yang lebih rendah dari tanaman kontrol (0 Gy). Hasil penelitian ini menunjukkan bahwa pemuliaan mutasi dengan teknik in vitro dapat menghasilkan perubahan morfologi dan juga peningkatan variabilitas sifat kuantitatif dan sejumlah parameter tersebut diharapkan dapat diaplikasikan untuk karakterisasi variasi kultivar pisang lainnya.Kata kunci : Musa acuminata (AAA), densitas stomata, dosis iradiasi sinar gamma

AKTIVITAS Stenotrophomonas rhizophila DAN Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Ganoderma boninense Bedah Rupaedah; Debby Viola Amanda; Reni Indrayanti; Nia Asiani; Bambang Sukmadi; Asep Ali; Abdul Wahid; Taufik Firmansyah; Mahmud Sugianto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Activities of Stenotrophomonas rhizophila and Trichoderma sp. in Inhibiting the Growth of Ganoderma boninenseÂ ABSTRACTBasal stem rot (BSR) disease in oil palm (Elaeis guineensis Jacq.) due to infection of Ganoderma boninense. Various efforts to overcome BSR disease has been done, such as by utilizing endophytic microbes. The purpose of this research was to determine the activities of Stenotrophomonas rhizophila and Trichoderma sp inhibiting the growth of G. boninense. This research was divided into three stages, namely: stability test of S. rhizophila activity against G. boninense; activity of chitinase and cellulase enzymes produced by S. rhizophila; the effectiveness of S. rhizophila and Trichoderma sp. on G. boninense in a greenhouse. The parameters observed were plant height, leaves number, chlorophyll content, disease incidence and severity. The stability testing of S. rhizophila activity against G. boninense showed 53% of inhibition. Chitinase activity showed negative result. While cellulase index was about 0.46. The effectiveness test showed the significantly different results on plant height, leaves number and chlorophyll content.Keywords: Chitinase, cellulase, Ganoderma boninense, Stenotrophomonas rhizophila, Trichoderma sp.Â ABSTRAKPenyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit (Elaeis guineensis Jacq.) muncul karena diinfeksi oleh Ganoderma boninense. Berbagai upaya penanggulangan penyakit BPB telah dilakukan, diantaranya dengan memanfaatkan mikroba endofit. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas bakteri Stenotrophomonas rhizophila dan Trichoderma sp. dalam menghambat pertumbuhan G. boninense. Penelitian ini dibagi menjadi tiga tahapan, yaitu: pengujian stabilitas aktivitas S. rhizophila terhadap G. boninense; pengujian aktivitas enzim kitinase dan selulase yang dihasilkan oleh S. rhizophila; pengujian efektivitas S. rhizophila dan Trichoderma sp. terhadap G. boninense di rumah kaca. Parameter yang diamati pada pengujian efektivitas berupa tinggi tanaman, jumlah daun, jumlah klorofil, kejadian dan keparahan penyakit. Uji stabilitas aktivitas S. rhizophila terhadap G. boninense menunjukkan adanya penghambatan rata-rata sebesar 53%. Uji aktivitas enzim kitinase pada bakteri S. rhizophila menunjukkan hasil negatif. Sedangkan indeks enzim selulase pada bakteri S. rhizophila sebesar 0.46. Pada uji efektivitas tampak hasil yang berbeda nyata pada parameter tinggi tanaman, jumlah daun dan kandungan klorofil.Kata Kunci: Kitinase, selulase, Ganoderma boninense, Stenotrophomonas rhizophila, Â Â Â Trichoderma sp.

Karakterisasi Sifat Toleransi terhadap Cekaman Kering Kacang Tanah (Arachis hypogega L.) Varietas Nasional pada Tahap Perkecambahan Adisyahputra Adisyahputra; Settings Reni Indrayanti; Dwi Eldina
Jurnal Matematika Sains dan Teknologi Vol. 5 No. 1 (2004)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.387 KB)

The aim of this research is to determine character for drought tolerance character prediction of peanut national variety on germination phase using PEG 6000 solution. Preliminary test using drought tolerance genotipes (US 605 and US 693), susceptible genotipe (PI 409) conducted to evaluate appropriate concentration of PEG solution as drought treatment. PEG 10% is appropriate for drought treatment. Experiment using factorial random complete design with eight national varieties, Badak, Gajah, Jerapah, Kelinci, Komodo, Macan, Panther, Singa, and PEG solution. Minimum water uptake for germination is obtained from proportion between seedling weight to seed weight with seed weight. Root length, number of lateral root and seedling dry weight (without cotyledon) are counted on seventh day after germination. Seed germinated using UKDdp method. ANOVA two way for water uptake variable, ANOVA one way for root length and number of lateral root and seedling dry weight (without cotyledon) is used to analyze data, continue with DMRT and Pearson product moment correlation between minimum water uptake for germination and root length, seedling dry weight (without cotyledon). And Spearman correlation is used between minimum water uptakes for germination with number of lateral root. The results base on this research are, drought tolerance characterization of peanut national variety could evaluate on germination phase simulated by 10% PEG 6000 solution. (2) Minimum water uptake for peanut seed germination could be use as determine character to drought (3) Base on minimum water uptake for germination, Gajah and Panther grouped as drought tolerance varieties, Macan, Jerapah, Singa and Badak as medium tolerance varieties, and Komodo and kelinci as susceptible varieties.(4) In peanut, root length, number of lateral root and seedling dry weight (without cotyledon) can not be use as determine character to drought on germination phase.

Isolation and Characterization of Potential Isolates of Rhizosphere Bacteria to Contro Budok Disease in Patchouli Plant NFN Sukamto; Novi Listiana; Reni Indrayanti; Dono Wahyuno
Buletin Penelitian Tanaman Rempah dan Obat Vol 30, No 1 (2019): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v30n1.2019.35-46

Synchytrium pogostemonis, the causal agent of budok disease, is one of the major limiting factors in patchouli production in Indonesia. Patchouli farmers usually control budok disease with chemical fungicides. Chemical control pollutes environment and disrupts agricultural ecosystem. Therefore, an environmentally friendly pest control should be conducted to control the disease. The objective of the study was to isolate and evaluate some rhizobacteria from the rhizosphere of patchouli and black pepper plants against Fusarium oxysporum f.sp. vanillae, F. solani, Sclerotium rolfsii. Potential rhizobacterial isolates were tested to control budok disease on a pot scale. The results showed that 26 rhizobacterial isolates from 100 tested were antagonistic to F. oxysporum, F. solani and S. rolfsii. Four rhizobacteria isolates (RL13-A, RL31-A, RL35-A, RL32-A) showed strong inhibition (>40 %) against the 3 pathogens. In polibag experiment, RL35-A, PS9, RL13-A, RL32-B, RL31-A isolates were able to suppress budok disease significantly by 84.01; 76.00; 65.99; 43.99; and 21.98 % respectively. These results indicated that RL35-A isolates have strong antagonistic effect compared to other isolates. Based on 16S rDNA analysis, RL35-A isolates possessed close relationship (99 %) with all species of Enterobacter sp. The antibiotic compound extracted from RL35-A culture broth using GC-MS analysis was identified as phenol, 2,6-dimethoxy-(canola). These results suggested that Enterobacter sp. was potential to be developed as biological agent for controlling budok disease in patchouli plants.

VIRULENSI DAN EFEKTIFITAS FILTRAT KULTUR F. oxysporum f.sp cubense ISOLAT BANYUWANGI UNTUK PENGUJIAN KETAHANAN PISANG AMPYANG TERHADAP LAYU FUSARIUM Reni Indrayanti; , Sudarsono
Zuriat Vol 22, No 1 (2011)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v22i1.6840

Fusarium oxysporum f.sp. cubense (Foc) isolate Banyuwangi merupakan cendawan patogen yang menginfeksi tanaman abaka (Musa textilis Nee). Virulensi dan patogenitas isolat ini terhadap pisang meja cv. Ampyang (Musa acuminata, AAA) belum diketahui. Tujuan percobaan ini adalah (1) menguji virulensi miselium biotropik Foc isolat Banyuwangi terhadap pisang cv. Ampyang secara in vitro dengan menggunakan metode kultur ganda (dual culture method); (2) mendapatkan konsentrasi filtrat kultur (FK) Foc isolat Banyuwangi yang efektif untuk digunakan sebagai agen penyeleksi pengujian seleksi in vitro pada plantlet pisang. Hasil percobaan virulensi Foc isolate Banyuwangi terhadap pisang Ampyang menunjukkan bahwa plantlet pisang yang dikulturkan bersamaan dengan mycelia Foc secara in vitro memperlihatkan adanya klorosis dan gejala kelayuan daun pada 10 hari setelah inokulasi. Plantlet mengalami kematian rata-rata pada usia 30.44 hari. Hal ini menunjukkan bahwa Foc isolat Banyuwangi virulen terhadap pisang Ampyang. Metode kultur ganda diyakini merupakan metode yang sederhana, mudah dan cepat serta efektif untuk skrining awal virulensi dan patogenitas Foc terhadap pisang. Hasil pengujian efektivitas filtrat kultur Foc sebagai agen penyeleksi menunjukkan bahwa efek penghambatan filtrat kultur Foc pada tunas pisang cv. Ampyang berada pada konsentrasi 40-60% (v/v). Pada konsentrasi tersebut 60.75 – 82.50% tunas mengalami kematian, sehingga diketahui bahwa filtrat kultur Foc isolate Banyuwangi cukup efektif sebagai agen penyeleksi untuk pengujian seleksi in vitro ketahanan tanaman pisang terhadap layu Fusarium.

SELEKSI IN VITRO UNTUK MENDAPATKAN TUNAS PISANG AMPYANG HASIL IRADIASI GAMMA INSENSITIF FILTRAT KULTUR F. oxysporum f.sp cubense Reni Indrayanti; Nurhajati A. Mattjik; Asep Setiawan; , Sudarsono
Zuriat Vol 23, No 1 (2012)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v23i1.6868

Pisang cv. Ampyang (Musa acuminata, AAA, subgroup non-Cavendish) merupakan jenis pisang meja, dan keberadaannya ini sudah sulit dijumpai karena diduga rentan terhadap penyakit layu Fusarium. Tujuan percobaan ini adalah untuk mendapatkan varian plantlet pisang cv. Ampyang insensitif filtrat kultur (FK) Foc melalui seleksi in vitro secara bertingkat. Pendekatan percobaan dilakukan dengan menyeleksi plantlet varian pisang hasil mutasi induksi dengan iradiasi gamma (20, 25, 30, 40, 45 dan 50 Gy) dalam media selektif mengandung FK Foc secara bertingkat (30%, 40%, 50% dan 60%). Kultur filtrat yang berasal dari cendawan Fusarium oxysporum f.sp. cubense (Foc) isolat Banyuwangi digunakan sebagai agen penyeleksi Tunas pisang in vitro yang mampu bertahan hidup dalam medium selektif diidentifikasikan sebagai tunas yang insensitif terhadap filtrat kultur Foc. Hasil penelitian ini menunjukkan bahwa seleksi in vitro pada media selektif mengandung filtratekultur Foc 30%, belum mampu menghambat pertumbuhan tunas varian yang diseleksi. Peningkatan konsentrasi filtrat kultur Foc sampai 50% hanya mampu menghambat beberapa perlakuan plantlet varian hasil iradiasi. Penghambatan pertumbuhan tunas secara signifikan terlihat pada media selektif mengandung filtrat kultur Foc 60%, dan hasil percobaan ini diperoleh 1695 plantlet (57.7%) teridentifikasi insensitif terhadap FK Foc. Plantlet-plantlet tersebut akan digunakan sebagai plasma nutfah untuk mendapatkan klon-klon tanaman pisang cv. Ampyang resisten layu Fusarium.

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