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Fauziah, Sarah Sabilla
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Agriculture, Biological Sciences & Forestry Chemistry Decision Sciences, Operations Research & Management Education Environmental Science

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Diagnosa Agen Penyakit Ikan di Kecamatan Cibereum, Sukabumi, Jawa Barat Ramadhani, Dian Eka; Pratiwi, Rifqah; Gultom, Novayanti Magdalena; Hakim, Rafi Fathul; Hapsari, Monic; Alhaq, Sofyan; Maula, Athaya; Fauziah, Sarah Sabilla; Hafid, Muhammad Erlan; Nurrafa, Nazla Wafi
JURNAL MEGAPTERA Vol 2, No 2 (2023): Jurnal Megaptera (JMTR)
Publisher : Politeknik Kelautan dan Perikanan Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jmtr.v2i1.13826

Abstract

Penelitian ini bertujuan untuk mendiagnosis agen penyakit ikan yang dibudidayakan di Kecamatan Cibereum, Sukabumi. Penelitian ini merupakan jenis survei lapangan dengan menggunakan metode observasi melalui survei dan wawancara pada lokasi yang ditentukan, serta melakukan pengamatan sampel di laboratorium untuk pemeriksaan agen penyakit, kemudian data dikumpulkan lalu dianalisis secara deskriptif. Pemeriksaan agen penyakit meliputi diagnosis level 1 berdasarkan histori berlangsungnya kegiatan budidaya melalui pengamatan langsung dan wawancara dengan pembudidaya. Sedangkan diagnosis level 2 yaitu pemeriksaan secara mikrobiologi di laboratorium. Jenis ikan yang diperiksa yaitu ikan nila Oreochromis niloticus dan ikan gurami Osphronemus goramy yang berasal dari 5 lokasi budidaya. Parameter yang diamati diantaranya prevalensi, intensity, mean abundance, mean intensity ektoparasit dan endoparasit, pemeriksaan jenis bakteri, dan pemeriksaan darah ikan, dan kemudian data dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa diagnosis level 1 menunjukkan bahwa ikan yang dibudidayakan menunjukkan abnormalitas diantaranya tubuh ikan berwarna pucat, terdapat pendarahan, muncul jamur pada sisik ikan, warna insang tidak normal dan bergerak soliter di permukaan air di pojokan kolam. Hasil diagnosis level 2 menunjukkan bahwa bakteri Flavobacterium, Cardiobacterium, dan Bordetella pertusis terdapat pada semua sampel yang diperiksa. Pemeriksaan parasit menunjukkan hasil prevalence (P) terhadap parasit Tricodina sp., Oodinium, Gyrodactylus Dactylogyrus 100%, intensity (I) 25-56, mean intensity (MI) 25.2, dan mean abundance (MA) 25.2. Hasil pemeriksaan darah menunjukkan bahwa jumlah sel darah merah (SDM) berkisar 8.49 x 105 sel/mm3 , hemoglobin antara 8-22%g, dan hematokrit 0.16- 0.35%. Berdasarkan hasil diagnosis level 1 dan 2 pada lima sampel di lokasi budidaya di Cibereum dinyatakan bahwa ikan dalam kondisi sakit.This research aims to diagnose disease agents in fish cultivated in Cibereum District, Sukabumi. This research is a type of field survey using observation methods through surveys and interviews at specified locations, as well as observing samples in the laboratory to examine disease agents, and then the data collected is analyzed descriptively. Examination of disease agents includes level 1 diagnosis based on the history of cultivation activities through direct observation and interviews with cultivators. Meanwhile, level 2 diagnosis is a microbiological examination in the laboratory. The types of fish examined were tilapia Oreochromis niloticus and gourami Osphronemus goramy which came from 5 cultivation locations. The parameters observed included prevalence, intensity, mean abundance, mean intensity of ectoparasites and endoparasites, examination of bacterial types, and examination of fish blood, and then the data was analyzed descriptively. The research results showed that a level 1 diagnosis showed that the fish being farmed showed abnormalities including pale fish bodies, bleeding, fungus appearing on the fish scales, abnormal gill color, and solitary movement on the surface of the water in the corner of the pond. Level 2 diagnosis results showed that Flavobacterium, Cardiobacterium, and Bordetella pertussis bacteria were present in all samples examined. Parasite examination showed that the prevalence (P) of Tricodina sp., Oodinium, Gyrodactylus Dactylogyrus parasites was 100%, intensity (I) 25-56, mean intensity (MI) 25.2, and mean abundance (MA) 25.2. The results of blood tests showed that the number of red blood cells (HR) was around 8.49 x 105 cells/mm3 , hemoglobin between 8-22%g, and hematocrit 0.16-0.35%. Based on the results of diagnosis levels 1 and 2 on five samples at the cultivation location in Cibereum, it was stated that the fish were sick.
Pengujian Obat Kimia untuk Menghambat Pertumbuhan Bakteri Patogen Aeromonas hydrophila Secara In Vitro dan In Vivo Kurniawinata, Mohamad Iqbal; Ramadhani, Dian Eka; Shinta, Deby; Bintoro, Abrisam Hadi; Indryani, Herlina; Wibisono, Adhitya; Permana, Shadam Dwi; Pamungkas, Riza; Pauziah, Nisa; Maula, Athaya; Fauziah, Sarah Sabilla; Hafid, Muhamad Erlan; Nurrafa, Nazla Wafi; Hapsari, Monic; Pratiwi, Rifqah
JURNAL MEGAPTERA Vol 3, No 2 (2024): Jurnal Megaptera (JMTR)
Publisher : Politeknik Kelautan dan Perikanan Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jmtr.v3i2.15151

Abstract

Penelitian ini bertujuan untuk mendapatkan jenis obat kimia dan dosis terbaik untuk menghambat pertumbuhan bakteri patogen Aeromonas hydrophila (AHA). Penelitian ini dilakukan di Laboratorium Kesehatan dan Lingkungan Sekolah, Program Studi Teknologi dan Manajemen Pembenihan Ikan, Sekolah Vokasi IPB. Rancangan percobaan yang dilakukan dari penelitian ini terdiri dari 4 perlakuan, diantaranya, kontrol (K), garam ikan 5 g/L, 10 g/L, 15 g/L, 20 g/L, 25 g/L, 30 g/L, methylene blue 0,5 mL/L, 1,0 mL/L, 1,5 mL/L, 2 mL/L, 2,5 mL/L, 3 mL/L, lukastop 0,01 mL/L, 0,013 mL/L. Pengujian obat dilakukan secara in vitro dengan pengujian tunggal dan gabungan masing-masing obat. Pengujian in vivo dilakukan dengan dosis terbaik dari hasil in vitro diujikan pada ikan sebagai pengobatan pada infeksi bakteri AHA. Parameter in vitro yang diamati yaitu zona hambat dan total bakteri patogen Aeromonas hydrophila, sedangkan parameter in vivo yang diamati yaitu Survival Rate (SR), Laju Pertumbuhan Harian (LPH) dan Mortality Rate (MR). Hasil pengujian in vitro pada pengujian tunggal yang terbaik didapatkan pada perlakuan methylene blue 3 mL/L dengan zona hambat sebesar 18,25 mm dan jumlah bakteri AHA terendah yaitu 5,197 × 109 CFU/mL. Hasil pengujian in vitro pada pengujian gabungan yang terbaik didapatkan pada perlakuan garam ikan 25 g/L dan methylene blue 3 mL/L dengan zona hambat 17,9 mm. Hasil pengujian in vivo pengobatan dengan methylene blue 3 mL/L, menghasilkan nilai SR 80% dan mampu membantu penyembuhan pasca infeksi serta meningkatkan nafsu makan ikan.This study aims to obtain the best type of chemical drug and dosage to inhibit the growth of the pathogenic bacteria Aeromonas hydrophila (AHA). This study was conducted at the School Health and Environment Laboratory, Fish Seed Technology and Management Study Program, IPB Vocational School, Sukabumi Campus. The experimental design carried out in this study consisted of 4 treatments, including control (K), fish salt 5 g/L, 10 g/L, 15 g/L, 20 g/L, 25 g/L, 30 g/L, methylene blue 0,5 mL/L, 1,0 mL/L, 1,5 mL/L, 2,0 mL/L, 2,5 mL/L, 3,0 mL/L, lukastop 0,01 mL/L, 0,013 mL/L. Drug testing was carried out in vitro with single and combined testing of each drug. In vivo testing was carried out with the best dose from the in vitro results tested on fish as a treatment for AHA bacterial infections. The observed in vitro parameters were the inhibition zone and total pathogenic bacteria Aeromonas hydrophila, while the observed in vivo parameters were survival rate (SR), daily growth rate (LPH), and mortality rate (MR). In vitro tests showed that methylene blue 3 mL/L treatment had the best results on a single test. The inhibition zone was 18,25 mm, and the number of AHA bacteria in the sample was 5,197 × 109 CFU/mL. The treatment of 25 g/L fish salt and 3 mL/L methylene blue yielded the best in vitro test results, with an inhibition zone of 17,9 mm. The results of vivo therapy testing with 3 mL/L methylene blue produced an SR value of 80% and were able to aid in post-infection healing and increase fish appetite.
Uji Efektivitas Antibiotik Cyprofloxacin, Enrofloxacin, Oxytetracycline Untuk Menghambat Bakteri Patogen Aeromonas Hydrophila Secara In Vitro Dan In Vivo Ramadhan, Dian Eka; Ansori, M Isya Rais; Maulana, Moch Rega; Putri, Meisya Meliana; Anisa, Anisa; Juliansyah, Ridho; Juliani, Risma Rosyada; Zahra, Ghaida Refiana; Anjani, Resti Dwi; Tama, Muhammad Lutfi Hauzan; Maula, Athaya; Fauziah, Sarah Sabilla; Djunaedi, Muhamad Erlan Hafid; Nurrafa, Nazla Wafi; Kurniawinata, Mohamad Iqbal
JURNAL MEGAPTERA Vol 4, No 1 (2025): Jurnal Megaptera (JMTR)
Publisher : Politeknik Kelautan dan Perikanan Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jmtr.v4i1.15149

Abstract

Penelitian ini bertujuan untuk memperoleh jenis antibiotik dan dosis terbaik dalam menghambat bakteri patogen Aeromonas hydrophila (AHA). Penelitian dilakukan di Laboratorium Kesehatan dan Lingkungan Program Studi Teknologi dan Manajemen Pembenihan Ikan, Sekolah Vokasi IPB Sukabumi. Penelitian menggunakan metode tunggal dengan dua ulangan dan 10 perlakuan, yaitu: kontrol (K), P1 ciprofloxacin dosis 0,01 ppm, P2 ciprofloxacin dosis 0,02 ppm, P3 ciprofloxacin dosis 0,03 ppm, P4 enrofloxacin dosis 10 ppm, P5 enrofloxacin dosis 15 ppm, P6 enrofloxacin dosis 20 ppm, P7 oksitetrasiklin dosis 40 ppm, P8 oksitetrasiklin dosis 50 ppm, dan P9 oksitetrasiklin dosis 60 ppm. Pada metode kombinasi, digunakan dua ulangan dengan empat perlakuan, yaitu tiga perlakuan terbaik dari metode tunggal dan satu kontrol: K (kontrol), P1 ciprofloxacin dosis 3000 ppm + oksitetrasiklin dosis 60 ppm, P2 enrofloxacin dosis 10 ppm + ciprofloxacin dosis 0,003 ppm, dan P3 oksitetrasiklin dosis 60 ppm + enrofloxacin dosis 10 ppm. Sementara itu, pada metode ko-kultur digunakan dua ulangan dengan empat perlakuan, yaitu: P0 (kontrol/AHA), P2 enrofloxacin dosis 10 ppm, P3 ciprofloxacin dosis 3000 ppm, dan P4 oksitetrasiklin dosis 60 ppm. Parameter yang diamati meliputi zona hambat dan total jumlah bakteri patogen. Berdasarkan hasil penelitian, antibiotik ciprofloxacin pada dosis tunggal 0,003 ppm menunjukkan efektivitas paling tinggi terhadap bakteri patogen Aeromonas hydrophila, dengan zona hambat sebesar 18,4 mm. Pada metode kultur gabungan, ciprofloxacin juga terbukti paling efektif terhadap bakteri AHA, dengan hasil total plate count (TPC) terendah sebesar 5,7 × 10⁵ CFU/ml. Uji in vivo dengan ciprofloxacin dosis 3000 ppm menghasilkan tingkat kelangsungan hidup (SR) sebesar 55%.This study aimed to determine the most effective type and dose of antibiotics to inhibit the pathogenic bacterium Aeromonas hydrophila. The research was conducted at the Health and Environmental Laboratory of the Fish Hatchery Technology and Management Study Program, IPB Sukabumi Vocational School. The study used a single-treatment method with two replications and ten treatments, namely: control (K), P1 ciprofloxacin at a dose of 0.01 ppm, P2 ciprofloxacin at 0.02 ppm, P3 ciprofloxacin at 0.03 ppm, P4 enrofloxacin at 10 ppm, P5 enrofloxacin at 15 ppm, P6 enrofloxacin at 20 ppm, P7 oxytetracycline at 40 ppm, P8 oxytetracycline at 50 ppm, and P9 oxytetracycline at 60 ppm. In the combination method, two replications were used with four treatments: three best treatments from the single method and one control, namely: K (control), P1 ciprofloxacin at 3000 ppm + oxytetracycline at 60 ppm, P2 enrofloxacin at 10 ppm + ciprofloxacin at 0.003 ppm, and P3 oxytetracycline at 60 ppm + enrofloxacin at 10 ppm. Meanwhile, in the coculture method, two replications and four treatments were applied: P0 (control/AHA), P2 enrofloxacin at 10 ppm, P3 ciprofloxacin at 3000 ppm, and P4 oxytetracycline at 60 ppm. The observed parameters included the inhibition zone and total pathogenic bacterial count. Based on the results, ciprofloxacin at a single dose of 0.003 ppm was the most effective against Aeromonas hydrophila, with an inhibition zone of 18.4 mm. In the combination culture method, ciprofloxacin also proved to be the most effective against AHA bacteria, with the lowest total plate count (TPC) result of 5.7 × 10⁵ CFU/ml. The in vivo test using ciprofloxacin at a dose of 3000 ppm resulted in a survival rate (SR) of 55%.
Co-Authors Alhaq, Sofyan Anisa Anisa Anjani, Resti Dwi Ansori, M Isya Rais Bintoro, Abrisam Hadi Djunaedi, Muhamad Erlan Hafid Gultom, Novayanti Magdalena Hafid, Muhamad Erlan Hafid, Muhammad Erlan Hakim, Rafi Fathul Hapsari, Monic Indryani, Herlina Iqbal Kurniawinata, Mohamad Juliani, Risma Rosyada Juliansyah, Ridho Maula, Athaya Maulana, Moch Rega Nurrafa, Nazla Wafi Pauziah, Nisa Permana, Shadam Dwi Pratiwi, Rifqah Putri, Meisya Meliana Ramadhan, Dian Eka Ramadhani, Dian Eka Riza Pamungkas, Riza Shinta, Deby Tama, Muhammad Lutfi Hauzan Wibisono, Adhitya Zahra, Ghaida Refiana