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All Journal Kenanga : Journal of Biological sciences and Applied Biology
Desy Nurrahma Dhani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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PENGGUNAAN EKSTRAK UMBI GADUNG UNGU (Dioscorea hispida) SEBAGAI PERWARNA ALTERNATIF PREPARAT SEDIAAN APUSAN DARAH TEPI (SADT) Ayu Nirmala Sari; Desy Nurrahma Dhani; Alifa Tazkiya; Ananda Wahyu Rizkina; Febri Elvisa
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 3 No. 2 (2023): Oktober 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v3i2.4212

Abstract

Pewarnaan sediaan apusan darah tepi (SADT) pewarnaan yang digunakan untuk ekstraksi antosianin dengan berbagai varian kosentrasi menunjukkan kualitas gambar terbaik. Kemungkinan hal ini disebabkan karena jumlah senyawa antosionin sangat berinteraksi dengan sel darah eritrosit, leukosit dan trombosit secara baik. Tujuan dari penelitian ini agar bisa melihat morfologi sel darah eritrosit, leukosit dan trombosit pada manusia. Hasil penelitian yang telah diui terdapat pada pewarnaan alternatif alami umbi gadung ungu (Dioscorea hispida) dimana terdapat senyawa antosionin untuk pewarnaan, umbi gadung menunjukkan warna ungu ke pink dan terlihat jelas sel darah trombosit, leukosit dan eritrosit, sedangkan pada Giemsa menunjukkan warna biru pudar dtetapi masih terlihat jelas sel darah trombosit, leukosit dan eritrosit.
PEWARNA ALTERNATIF PREPARAT SEDIAAN APUSAN DARAH TEPI (SADT) DARI EKSTRAK BUNGA KAMBOJA (Plumeria rubra L. cv. Acutifolia.) Ayu Nirmala Sari; Desy Nurrahma Dhani; Alifa Tazkiya; Badratun Nafis; Siti Diah Ayu Lestari Gea; Rizki Fazilah
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 4 No. 1 (2024): April 2024
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

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Abstract

Peripheral Blood Smear Preparation (SADT) is a smear preparation made using human blood that is colored for examination in hematology. This examination is done to see and assess peripheral blood cells such as leukocytes, platelets and erythrocytes. Giemsa has a drawback because methylene blue contained in giemsa can be inhaled causing cyanosis. Azur B content can also cause waste because the material contained is not easily decomposed and eosin content can cause allergic reactions.This study aimed to test the water extract of Cambodian flowers (Plumeria rubra L. cv. Acutifolia.) as an alternative dye of peripheral blood smear preparations. This research is in the form of qualitative research with a method of making purple dye for water extraction of frangipani flowers (Plumeria rubra L. cv. Acutifolia.). Microscopic observations of frangipani flower extract (Plumeria rubra L. cv. Acutifolia.) as a preparation of Edge Blood Smear (SADT) can be used as an alternative natural use besides Giemsa staining, because erythrocytes, leokocytes and platelets are able to bind colors well.
Co-Authors Alifa Tazkiya Ananda Wahyu Rizkina Ayu Nirmala Sari Badratun Nafis Febri Elvisa Rizki Fazilah Siti Diah Ayu Lestari Gea