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Testing the Anti-Bacterial Activity of Star Gooseberry Leaves (Souropus Androgynus L) Using the Disc Diffusion Method Using the n-Hexane Fraction Against Propionic Bacterium Acnes Devita Riafinola Andaririt; Ardi Broto
Strada Journal of Pharmacy Vol. 5 No. 2 (2023): October
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjp.v5i2.100

Acne vulgaris or acne, is a common inflammatory condition in the polysebaceous unit that occurs in teenagers and young adults and is characterized by comedones, papules, pustules, and nodules. One of the factors that causes acne is the Propionic bacterium acnes bacteria. One type of plant with antibacterial properties for acne is the Star Gooseberry Leaf plant (Souropus Androgynus L), which contains alkaloids, saponins, tannins, and flavonoids that act as antibacterials. Susanti (2012). This research aimed to obtain data on the antibacterial activity of the compound components contained in the n-hexane fraction of Star Gooseberry Leaves (Souropus Androgynus L) against the Propionic bacterium acnes bacteria, which can be seen from the inhibition zone. The method used was disc diffusion with three groups of control, namely (K(S), K(+), and K(-)). The test bacteria Propionic bacterium acnes, where the Control Sample consists of 4 test concentrations, namely 30 mg, 60 mg, 120 mg, and 240 mg. The positive control used two µg/disc Clindamycin, while the negative control was 2% DMSO. The results of the disc diffusion test of the ethanol fraction of Star Gooseberry Leaves (Souropus Androgynus L) using the disc diffusion method with concentrations of 30 mg/ml and 60 mg/ml did not provide an inhibition zone, the concentration of 120 mg/ml was 11.1 ± 0.10 mm, the concentration was 240 mg/ml was 14.63 ± 0.25 mm. The negative control DMSO 2% did not provide an inhibitory zone diameter, whereas the positive control Clindamycin 2 µg/disk provided an inhibition zone of 25.47 ± 0.70 mm.

Analysis of Factors Influencing Pharmacist Compliance in Reporting Side Effects of Medications in District Health Center X Pebrian, Nggio; Hanie Kusuma Wardani; Ardhi Broto Sumanto; Hanik Maria Dewi
Strada Journal of Pharmacy Vol. 7 No. 1 (2025): April
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjp.v7i1.174

The occurrence of Adverse Drug Reactions (ADR) cases has resulted in an increase in the number of patients experiencing pain and death, both when patients are in the hospital and in everyday life. The purpose of this study was to analyze the factors that influence pharmacist compliance in reporting adverse drug reactions at Health Center X. This study used a quantitative research type with a cross-sectional research design. The study population was all pharmacists at Health Center X with a sample size of 25 respondents. The independent variables were knowledge, attitudes, availability and accessibility, influence of colleagues and rewards, availability of facilities and policies/regulations related to ADR reporting and the dependent variable was practices related to ADR reporting. Data collection used a questionnaire and was analyzed using the Spearman test. From the results of the study, it is known that the significance value of knowledge is 0.351>0.05, the significance value of attitude is 0.545>0.05, the significance value of availability and accessibility is 0.586>0.05, the significance value of availability of facilities is 0.413>0.05, the significance value of ADR reporting policy is 0.127>0.05, meaning that there is no influence between the variables of knowledge, attitude, availability and accessibility, availability of facilities and ADR reporting policy on pharmacist compliance at health centers in District X. While the significance value of the influence of coworkers and rewards is 0.012<0.05, meaning that there is an influence between the variables of the policy of the influence of coworkers on pharmacist compliance at health centers X.

Formulation of Fibrauren tinctoria Lour. Extract Serum, As an Antioxidant with the 1,1-Diphenyl 2-Picryhydrazyl (DPPH) Method Wijayanto, Arif; Herdianty, Juvita; Putri, Luluk Aniqoh Meliana; Sumanto, Ardhi Broto
Open Access Health Scientific Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Griya Eka Sejahtera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55700/oahsj.v6i2.125

Background: Serum is a preparation with a high concentration of active ingredients and low viscosity, which delivers a thin film of active ingredients to the skin surface. The use of Fibraurea tinctoria has been carried out since ancient times by the Dayak, Banjar, and Kutai tribes as a traditional treatment for liver disease, diabetes, and to increase the body's immunity from various causes of disease such as bacteria. Fibraurea tinctoria is believed to be able to ward off the negative effects caused by free radicals such as antioxidants.Methods: This experimental study investigated the antioxidant activity of Fibraurea tinctoria extract serum with varying concentrations of hydroxyethyl cellulose 1.0%, 1.5%, and 2.0% using the DPPH methods, which is simple, easy, fast, and sensitive, and requires a small sample. This method provides information on the reactivity of the tested compound with a stable radical. Evaluations of the Fibraurea tinctoria extract serum preparation was carried out by observing the results of physical quality tests, including organoleptic tests, homogeneity tests, pH tests, adhesion tests, and spreadability tests. The serum preparation was tested for 3 days and stored at room temperature. Data were analyzed descriptively by comparing it with the Indonesian National Standard.Results: The results showed that the serum extract preparations of Fibraurea tinctoria F1st, F2nd, and F3rd showed varying IC₅₀ values, where lower values indicated stronger antioxidant potential. Formulation F3rd showed the lowest IC₅₀ value (3.11 ppm), F2nd (3.74 ppm) and F1st (4.67 ppm), all of which were stronger than the base (5.11 ppm).Conclusion: In this research, formulations F1st, F2nd, and F3rd had the highest antioxidant activity, with F3rd at a concentration of 2.0%. The identified strong antioxidant activity is the main basis for the potential of Fibraurea tinctoria extract serum as an active antioxidant ingredient.

Anti-Acne Activity Evaluation Of Cyperus rotundus L. Rhizome Fraction Gel Against Staphylococcus aureus And Propionibacterium acnes Qadariah, Musdalifah; Juvita Herdianty; Luluk Aniqoh Meliana Putri; Ardhi Broto Sumanto
Strada Journal of Pharmacy Vol. 7 No. 2 (2025): October
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjp.v7i2.203

Acne is an inflammatory skin condition often caused by bacteria such as Propionibacterium acnes and Staphylococcus aureus. The increasing incidence of antibiotic resistance encourages the search for alternatives from natural ingredients. Nut grass rhizome (Cyperus rotundus L.) is known to have antibacterial compounds. This study aimed to formulate a gel from the fractions of nut grass rhizome, test its activity against acne-causing bacteria, and identify the most potent fraction. This study employed a laboratory experimental design. The nut grass rhizome was extracted with 70% ethanol and then fractionated by liquid-liquid partitioning using n-hexane, ethyl acetate, and water as solvents. Each fraction was formulated into a gel at a 5% concentration, and its antibacterial activity was tested using the well diffusion method. All gel formulas met the physical quality evaluation requirements. The ethyl acetate fraction (F2) exhibited the strongest antibacterial activity, with an average inhibition zone of 19.48 ± 0.99 mm (moderate category) against Staphylococcus aureus and 25.86 ± 0.65 mm (strong category) against Propionibacterium acnes. The nut grass rhizome fraction gel preparation possesses anti-acne activity. The ethyl acetate fraction proved to be the most potent, showing efficacy against Propionibacterium acnes (inhibition zone 25.86 mm) that was statistically equivalent to the positive control (p = 0.767).

Investigation of the Anticancer Potential of Vernonia amygdalina Methanol Extract: A New Hope for MDA-MB-231 Breast Cancer Therapy Mesak, Ivan Junius; Sumanto, Ardhi Broto
Open Access Health Scientific Journal Vol. 7 No. 1 (2026): February 2026
Publisher : Griya Eka Sejahtera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55700/oahsj.v7i1.143

Background: MDA-MB-231 is a breast cancer cell line derived from metastatic adenocarcinoma and classified as a triple-negative breast cancer (TNBC) subtype. This breast cancer subtype is highly aggressive, has a high recurrence rate, and offers limited treatment options due to the absence of estrogen, progesterone, and HER-2 receptor expression. Therefore, the development of advanced therapeutic strategies is urgently needed to inhibit the metastasis of TNBC cancer cells. This study aimed to investigate the potential of the methanol extract of Vernonia amygdalina leaves as a natural chemotherapeutic agent for metastatic breast cancer therapy, with a focus on inducing cell death through apoptosis mechanisms. Methods: This study is an experimental research that began with the collection and identification of raw materials, followed by the preparation of simplicia, extraction processes, and phytochemical screening. Subsequently, cytotoxicity testing was conducted using the MTT assay, cell cycle analysis was performed using the PI-staining assay, and apoptosis was assessed using the Annexin V/PI-staining assay, all of which were analyzed through flow cytometry.Results: The methanol extract of Vernonia amygdalina demonstrated a moderate cytotoxic effect with an IC₅₀ value of 109.36 µg/mL. The extract induced S-phase cell cycle arrest in a dose-dependent manner, indicating its ability to reduce cell viability by inhibiting DNA replication. In the apoptosis assay, the highest percentage of cell death (3.12%) was observed at the concentration of 54.5 µg/mL, suggesting that this dose produced the strongest apoptotic response among the treatments tested. Conclusion: The methanol extract of Vernonia amygdalina leaves shows potential as a natural chemotherapeutic agent for metastatic breast cancer. The extract can induce cancer cell death through apoptosis, indicating its promise for further development as a supportive anticancer therapy.

Anti-Acne Activity Evaluation Of Cyperus rotundus L. Rhizome Fraction Gel Against Staphylococcus aureus And Propionibacterium acnes Qadariah, Musdalifah; Juvita Herdianty; Luluk Aniqoh Meliana Putri; Ardhi Broto Sumanto
Strada Journal of Pharmacy Vol. 7 No. 2 (2025): October
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/sjp.v7i2.203

Acne is an inflammatory skin condition often caused by bacteria such as Propionibacterium acnes and Staphylococcus aureus. The increasing incidence of antibiotic resistance encourages the search for alternatives from natural ingredients. Nut grass rhizome (Cyperus rotundus L.) is known to have antibacterial compounds. This study aimed to formulate a gel from the fractions of nut grass rhizome, test its activity against acne-causing bacteria, and identify the most potent fraction. This study employed a laboratory experimental design. The nut grass rhizome was extracted with 70% ethanol and then fractionated by liquid-liquid partitioning using n-hexane, ethyl acetate, and water as solvents. Each fraction was formulated into a gel at a 5% concentration, and its antibacterial activity was tested using the well diffusion method. All gel formulas met the physical quality evaluation requirements. The ethyl acetate fraction (F2) exhibited the strongest antibacterial activity, with an average inhibition zone of 19.48 ± 0.99 mm (moderate category) against Staphylococcus aureus and 25.86 ± 0.65 mm (strong category) against Propionibacterium acnes. The nut grass rhizome fraction gel preparation possesses anti-acne activity. The ethyl acetate fraction proved to be the most potent, showing efficacy against Propionibacterium acnes (inhibition zone 25.86 mm) that was statistically equivalent to the positive control (p = 0.767).

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