Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal ACTA VETERINARIA INDONESIANA ARSHI Veterinary Letters
Perdhana, Ika Satya
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Method Modification of Developing Ischemic Stroke Animal Models Using Intravenous Catheters in Rats Perdhana, Ika Satya; Handharyani, Ekowati; Darusman, Huda Shalahuddin; Nugroho, Setyo Widi; Maheshwari, Hera; Fitri, Arni Diana
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.67-76

Abstract

In various parts of the world, including Indonesia, the incidence of ischemic stroke remains high, and its impact poses a significant burden. This condition is influenced by numerous limitations in ischemic stroke therapy. Research on ischemic stroke is therefore crucial to develop, making the creation of appropriate animal models a vital necessity. One of the most commonly used methods by researchers to create animal models is the Middle Cerebral Artery Occlusion (MCAO) method. However, in Indonesia, its application remains limited due to the lack of available facilities. Method modifications can be implemented to enable the development of animal models in Indonesia. This study employed modifications to the MCAO method on eight Sprague Dawley rats. The modification involved using an intravenous catheter to facilitate the insertion of the filament as an occluder in the middle cerebral artery of the rats. Based on clinical and pathological observations, it can be concluded that the modified MCAO method used in this study is suitable for creating ischemic stroke animal models. With this modification, the creation of ischemic stroke animal models can be carried out in a simpler and more cost-effective manner.
Neurogical deficit score dan gambaran hematologi tikus model strok iskemik dengan metode middle cerebral artery occlusion Perdhana, Ika Satya; Hardiansyah, Muhamad Evans; Maheshwari, Hera; Mohamad, Kusdiantoro
ARSHI Veterinary Letters Vol. 9 No. 3 (2025): ARSHI Veterinary Letters - August 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.3.83-84

Abstract

Stroke is a neurological disorder caused by impaired blood flow to specific regions of the brain, often resulting in paralysis and functional deficits in the affected area. Among its subtypes, ischemic stroke is the most prevalent in the general population. This study aimed to establish a rat model of ischemic stroke induced by middle cerebral artery occlusion (MCAO). Eight male Sprague-Dawley rats aged 8–10 weeks were randomly assigned to two groups: control and treatment group. In the treatment group, the middle cerebral artery was occluded for 45 min. Neurological assessment using the Bederson scale revealed a neurological deficit score (NDS) of 1, characterized by contralateral flexion after left-brain injury. Hematological analysis demonstrated significantly higher white blood cell (WBC) counts (p < 0.05), whereas red blood cell (RBC) counts, hemoglobin concentration, and hematocrit values were significantly lower (p < 0.05) than those in the control group. These findings indicate that the MCAO method with a 45-minute occlusion successfully induced a mild neurological deficit accompanied by distinct hematological alterations, thereby providing a reproducible animal model for ischemic stroke research.
Co-Authors Ekowati Handharyani Fitri, Arni Diana Hardiansyah, Muhamad Evans HERA MAHESHWARI Huda Shalahudin Darusman Kusdiantoro Mohamad Nugroho, Setyo Widi