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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Current Biochemistry QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Halal Studies and Society
Dwicesaria, Maheswari Alfira
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Social Sciences Other

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Journal : Current Biochemistry

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Kandungan Nutrisi dan Daya Inhibisi α-glukosidase Ekstrak Daging Buah Salak Sidempuan (Salacca sumatrana) Safithri, Mega; Ritonga, Aprilita Putri Defan; Indariani, Susi; Dwicesaria, Maheswari Alfira; Sulistiyani; Hasim
Current Biochemistry Vol. 11 No. 1 (2024)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.11.1.1

Abstract

Penelitian terhadap pemanfaatan salak khususnya salak Sidempuan sebagai inhibitor α-glukosidase belum dilakukan. Penelitian ini bertujuan mengukur kadar proksimat (air, abu, protein kasar, lemak kasar, serat kasar, dan karbohidrat), menganalisis komponen fitokimia, kandungan total fenolik dan aktivitas penghambatan α-glukosidase pada ekstrak air dan etanol daging buah salak Sidempuan. Kadar total fenol diukur menggunakan metode Folin Ciocalteu. Aktivitas penghambatan α-glukosidase diukur menggunakan substrat pNPG. Kadar karbohidrat, air, abu, lemak kasar, protein kasar, dan serat kasar berturut-turut sebesar 86.35%, 6.57%, 3.60%, 1.44%, 1.24%, dan 0.82%. Kedua ekstrak mengandung senyawa flavonoid dan saponin. Ekstrak etanol 70% daging buah salak Sidempuan memiliki kandungan total fenol sebesar 10.6 mg GAE/g ekstrak, lebih tinggi dibandingkan ekstrak air sebesar 5.55 mg GAE/g esktrak. Ekstrak etanol daging buah salak Sidempuan memiliki konsentrasi inhibisi (IC50) sebesar 13.69 mg/L. Konsentrasi ini lebih kecil dibandingkan ekstrak air yaitu 4160.56 mg/L, namun masih lebih besar dibandingkan akarbosa yaitu 1.15x10-3 mg/L sebagai kontrol positif.
Antibacterial Activity of Hermetia illucens (Black Soldier Fly) Maggot Extract Against Salmonella typhi Andrianto, Dimas; Hasim; Muhammad, Zulfikar; Dwicesaria, Maheswari Alfira
Current Biochemistry Vol. 12 No. 2 (2025)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.12.2.1

Abstract

Typhus is an acute fever caused by Salmonella typhi bacterial infection. This research aims to test the efficacy of BSF maggot extract as an antibacterial against Escherichia coli and Salmonella typhi. The stages in this research are the preparation of maggot samples, determination of water content of samples, extraction of maggot BSF, screening of antibacterial activity of maggot BSF against Escherichia coli and Salmonella typhi, fractionation from the best results of antibacterial activity of maggot extract BSF and the test of antibacterial activity of maggot BSF fraction against Escherichia coli and Salmonella typhi, fractionation of the best results of antibacterial activity of maggot extract BSF and the test of antibacterial activity of maggot BSF fraction against Escherichia coli and Salmonella typhi. Extraction was carried out by a multi-level maceration method using acetone, methanol and water solvents, then the extract was tested for antibacterial activity against Salmonella typhi using the disc diffusion method with the best results, acetone extract with an inhibition zone of 8.13 mm ± 0.153. Then, acetone extract was fractionated according to its polarity, namely n-hexane, dichloromethane, ethyl acetate, and water. The results of the fractionation were tested for antibacterial activity against Escherichia coli and Salmonella typhi using the disc method with the best fraction was the n-hexane fraction, at a concentration of 2500 ppm with inhibition zones 8.37 mm ± 0.32 (Escherichia coli) and 9.00 mm ± 0.00 (Salmonella typhi)
Co-Authors Achyar, Catellia Auliany DIMAS ANDRIANTO Febrina, Adella Fitrilia, Tiana Gholam, Gusnia Meilin Hasim Hudayanti, Martini Indariani, Susi Kustiariyah Tarman Mega Safithri Nusyarah, Ayu Tri Purwanto, Ukhradiya Magharaniq Safira Rini Kurniasih, Rini Ritonga, Aprilita Putri Defan Rosyidah, Rara Annisaur Saputra, Rahadita Maura Amanda Silitonga, Ria Gusharani Sulistiyani Wong, Chen Wai Zulfikar Muhammad, Zulfikar