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All Journal Borneo Journal of Medical Laboratory Technology
Ramdhani , Fitria Hariati
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

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Journal : Borneo Journal of Medical Laboratory Technology

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Analisis Aktivitas Antibakteri Ekstrak Daun Kelor (Moringa oleifera Lam.) Terhadap Pertumbuhan Pseudomonas aeruginosa Rahmah, Windya Nazmatur; Ramdhani , Fitria Hariati; Hanida , Luthfiani; Sartika, Fera
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 2 (2025): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i2.9711

Abstract

Moringa leaves (Moringa oleifera Lam.) are known to have various health benefits, one of which is their potential as an antibacterial agent. This study aims to activate the antibacterial activity of moringa leaf extract against the growth of Pseudomonas aeruginosa. The test was carried out with five treatments, namely moringa leaf extract with concentrations of 15%, 30%, 50%, positive control, and negative control. The well diffusion method was used in the test, with incubation at 37°C for 24 hours. The diameter of the inhibition zone was measured as an indicator of effectiveness. The results showed that a concentration of 15% did not produce an inhibition zone, while concentrations of 30% and 50% showed an average inhibition zone of 0.87 mm and 2.85 mm, respectively. Based on the CLSI criteria, all results were divided into resistant because the size of the inhibition zone was ≤ 15 mm. Thus, it can be concluded that moringa leaf extract has not shown optimal effectiveness in inhibiting the growth of Pseudomonas aeruginosa..
Karakterisasi Imunogenesitas Protein Pilli Salmonella Typhi: Analisis Absorban Antibodi dan Spesifisitas dengan Western Blot pada Mencit Balb/C Hidayani, Al; Darmawati, Sri; Ramdhani , Fitria Hariati; Rahmah, Windya Nazmatur
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 2 (2026): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i2.12477

Abstract

Protein pili berperan dalam adhesi dan bersifat imunogenik, berpotensi sebagai kandidat vaksin subunit. Penelitian ini menganalisis imunogenesitas protein pili S. typhi isolat BA07.4 melalui pengukuran absorbansi antibodi dan karakterisasi spesifisitasnya. Protein pili diisolasi dengan metode biphasic, dimurnikan, dan digunakan untuk imunisasi 30 ekor mencit Balb/C (6 kelompok: kontrol dan dosis 2, 3, 4, 5, 6 µg) secara intraperitoneal tiga kali. Kadar antibodi diukur dengan inhouse ELISA (λ 405 nm) dan spesifisitasnya dianalisis dengan Western blot. Rerata absorbansi tertinggi pada dosis 5 µg (2,827), diikuti dosis 6 µg (2,678), 4 µg (2,456), 3 µg (2,134), 2 µg (1,856), dan kontrol (0,423). Analisis One Way ANOVA menunjukkan perbedaan signifikan antar kelompok (p=0,000) dengan semua kelompok perlakuan berbeda signifikan terhadap kontrol (p<0,05). Western blot menunjukkan pita spesifik pada berat molekul 87, 58, dan 42 kDa pada kelompok dosis 5 µg, sementara kontrol tidak menunjukkan pita. Protein pili S. typhi bersifat imunogenik dengan dosis optimum 5 µg, menghasilkan antibodi yang secara spesifik mengenali sub unit protein pili 87, 58, dan 42 kDa, mendukung potensinya sebagai kandidat vaksin subunit.
Co-Authors Fera Sartika Hanida , Luthfiani Hidayani, Al Sri Darmawati Windya Nazmatur Rahmah