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All Journal International Journal of Applied and Scientific Research (IJASR)
Obeagu E I
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Religion Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Social Sciences

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Histomorphological and Hormonal Changes in the Testes of Albino Rats Treated with Herbal Aphrodisiac Formular Wilson, Ajanwachukwu Ogbonna; Ohiwerei Wisdom Omogbai; Olugbenga Mary; Echekwube Marylyn E; Obeagu E I
International Journal of Applied and Scientific Research Vol. 2 No. 12 (2024): December 2024
Publisher : MultiTech Publisher

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Abstract

Herbal supplements, commonly referred to as botanicals, are plant-derived products utilized for their therapeutic properties. This study aims to examine the histomorphological and hormonal changes in the testes of albino rats treated with a herbal aphrodisiac formula. A total of eight male and two female rats were used in the study, with the males divided into control and test groups, while the females were used for mating with the test groups. Group A (Control) received only normal feed (growers’ mash) and distilled water daily for four weeks. Group B was administered 0.2 ml of Men’s Formula supplement and sacrificed on day 7, whereas Group C was administered 0.2 ml of Men’s Formula supplement and sacrificed on day 14. The body weights of the animals were measured before and after acclimatization, and similar weight assessments were performed at the end of each phase, with the average weights recorded accordingly. At the end of the procedure, the testes of each rat were excised under chloroform anesthesia and fixed in 10% formal saline for subsequent histological examination. Blood samples were collected via cardiac puncture into plain bottles, and sera were later separated for hormonal analysis. The results indicated that the herbal aphrodisiac formula enhanced testosterone levels and increased testis weight. However, no significant change (P > 0.05) was observed in body weight, and testicular histology remained unaffected. Further studies with prolonged usage of the herbal aphrodisiac formula are recommended to determine potential long-term effects
Histological Effect of Ultra Violet (UV) Light on the Testis of Albino Wistar Rats Uwuigbe Mathew; Ohiwerei Wisdom Omogbai; Ogbe O Clementina; Ajanwachukwu Ogbonna Wilson; Obeagu E I
International Journal of Applied and Scientific Research Vol. 2 No. 12 (2024): December 2024
Publisher : MultiTech Publisher

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Abstract

UV light, or ultraviolet light, is a type of radiation that falls between the visible light and X-ray regions of the electromagnetic spectrum. It is a form of electromagnetic radiation emitted by the sun and artificial sources, such as tanning beds and UV lamps. It has a shorter wavelength than visible light, ranging from 100 to 400 nanometers (nm). It is categorized into three types based on their wavelengths: UVA, UVB, and UVC. The aim of this study is to evaluate the histopathological effect of uv light on the testis of male Wistar albino rats. A total of fifty (50) adult Albino Wistar rats of comparable sizes were used for this study. They were divided into four equal groups (A – E) with ten (10) rats each. Group A served as the control and the rats were given distilled water and feed only. In addition to feed and water, Groups B rats were kept under Uv light for 30mins, Group C rats were kept under Uv light for 1hour twice daily, Group D rats were kept under Uv light for 2 hours thrice daily and Group E were kept under Uv light for 4 hours thrice daily. The administration was given daily for 14 days (2 weeks) and the weights of both the test and control animals was monitored before and after administration of Uv light. After the administration, the rats were put under light chloroform anaesthesia and the stomach was obtained. ANOVA was used to analyze the results of the weight and differences was considered significant at p<0.05 level of confidence. All data was expressed in table as mean ± standard deviation (SD). From the results, it was observed that Group B showed mild adenofibroma, Group C exhibited denatured testicular cells, indicating severe damage. Group D had adenocarcinoma and fatty tissue, suggesting potential malignancy and Group E presented fatty tissue. In conclusion, the exposure of testis to uv light cause significant alterations in testis histology
Co-Authors Ajanwachukwu Ogbonna Wilson Echekwube Marylyn E Ogbe O Clementina Ohiwerei Wisdom Omogbai Olugbenga Mary Uwuigbe Mathew Wilson, Ajanwachukwu Ogbonna