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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Nafis, Fardha Ad Durrun
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The Quality of Sexed Bali Bull Semen Using Percoll Density Gradient Centrifugation in Various Gradients with Tris Aminomethane Diluent Rahmawati, Nanda Ayu; Yekti, Aulia Puspita Anugra; Wahyudi, Aditiya; Nafis, Fardha Ad Durrun; Isnaini, Nurul; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.4

Abstract

This research aims to determine the quality of spermatozoa in the sexed semen that separated using the Percoll Density Gradient Centrifugation (PDGC) method with various gradients using egg yolk tris aminomethane diluent. The study was conducted at the Singosari Artificial Insemination Center from December 2023 to February 2024. The study's material was the semen of one Bali bull with individual motility ? 70%. The experimental design used a Randomized Group Design with the sexed semen PDGC method treatment: T1 = 10 gradients, T2 = 5 gradients, and T3 = 3 gradients, each with ten replications. The analysis showed that the gradient variations significantly impact the individual motility on the bottom layer of sexed semen (P<0.05). Conversely, gradient variance did not affect viability, abnormality, concentration, total motile spermatozoa, and individual motility on the top layer of sexed semen (P>0.05). In conclusion, sexed semen using the PDGC method with varied gradients is suitable for the freezing process due to its high quality.
Co-Authors Aulia Puspita Anugra Yekti Nurul Isnaini Rahmawati, Nanda Ayu Wahyudi, Aditiya