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All Journal Buletin Peternakan Jurnal Ilmu Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Kumawula: Jurnal Pengabdian Kepada Masyarakat REKASATWA : Jurnal Ilmiah Peternakan Livestock and Animal Research Jurnal Agripet

Aulia Puspita Anugra Yekti

Dosen Produksi Ternak Fakultas Peternakan, Universitas Brawijay

Author-ID : 2845970

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Social Sciences Veterinary Other

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Pengaruh pengencer air kelapa tua yang berbeda varietas terhadap kualitas semen cair Kambing Boer pada penyimpanan 3-50C Aziz, Achmad Fadhli; Salim, Muhammad Ade; Isnaini, Nurul; Yekti, Aulia Puspita Anugra; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 28, No 2 (2018): Agustus
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2018.028.02.03

Artificial Insemination using liquid semen is technology reproduction can improve goat productivity without liquid nitrogen. The Purpose of this research was to determine differences of old coconut water varieties of red (Cocos rubescens) and green (Cocos viridis) as diluent Boer goat semen, based on motility, viability and spermabnormalities during storage at 3-50C. The materials used for this research was Boer goat fresh semen as much as 3 heads were collected 2 times a week. Experimental Design. The treatment were devided into three Treatmen, there were: P0 (Tris+ 20% egg yolk); P1 (old coconut water varieties red + 20% egg yolk); P2 (old coconut water varieties green + 20% egg yolk). The data were analyzed by randomized block design, if the result are different or significantly different then continued to be tested with Duncan's multiple range test. The result showed that during storage until days 4, P0 has the best result on motility (40,5 + 1,1 %), viability (45,18+ 1,56 %), and abnormality (1,89+ 0,59 %). While on the diluent using old coconut water green variety better then red variety. On day of 2 P1 and P2 have motility (61 + 1,3%) and (51+ 7,5%); Viability (67,14+17,99%) and (55,37+10,66%), abnormality (1,23 + 1,02%) dan (1,70+ 0,99%).

Conception rate hasil inseminasi buatan menggunakan semen sexing beku pada Sapi Persilangan Ongole Yekti, Aulia Puspita Anugra; Kurniaesa, Titah Ulya; Isnaini, Nurul; Kuswati, Kuswati; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 28, No 3 (2018): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2018.028.03.07

Artificial Insemination (AI) is a technique that is widely used by farmers to improve the genetic quality of livestock. This method can be increased in value by using sexing semen that can regulate and produce the sex of the offspring as expected. The purpose of this study was to determine the success of AI by using frozen sexing semen on Ongole Crossbred cows in Malang Regency. The materials used were 98 Ongole crossbred cows in traditional farm of Malang Regency especially in Pakis, Tumpang, Jabung and Singosari Districts. The method used was a field experiment with two treatments: 49 cows were inseminated by using frozen sexing semen (P1) and 49 cows were inseminated by using non sexing semen (P0). The variables observed were the Non-Return Rate (NRR) value observedin 3 estrous cycle after AI and CR value based on pregnancy observation at 8 months of pregnancy. Data were analyzed descriptively and continued with a one-proportion z-test. The datashowed that the CR value on P1; P0 were 55.10% and 65, 31% respectively.furthemore, the NRR value on P1 from NRR1 to NRR3 were 87,76%, 73,47% and 67,35%, while on P0 95,92%, 83,67% and 75,51% respectively.It can be conclude thatCR and NRR value of sexing semenwas lower than non sexing semen, however sexing semen can be applied for certain purposes such as producing offspring as expected

The Quality of Frozen Semen with Different Thawing Duration and Temperature on Simmental Bull Nisa, Dhinda Choirun; Susilawati, Trinil; Yekti, Aulia Puspita Anugra; Rachmawati, Achadiah
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 32, No 1 (2022): April 2022
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2022.032.01.11

This study aimed to determine the accuracy of various thawing duration and temperatures to the quality of Simmental bull frozen semen. The experimental design used was a randomized group design with five treatments and ten replications, then analyzed using Chi-Square to compare the expected percentage of motility is 40%, and total motile spermatozoa are 10 million/straw value of SNI. A further test is carried out with Duncan's Multiple Range Test (DMRT) if there is a difference. The thawing treatments were 30 seconds at 37ËšC water temperatures (T0) according to SNI and at 15 seconds (T1), 30 seconds (T2), 45 seconds (T3), and 60 seconds (T4) with 28ËšC tap water temperatures. Variables observed involve motility, viability, abnormalities, concentration, and total motile spermatozoa. The data were analyzed using Analysis of Variance (ANOVA) with a significant difference in variance (P<0,05). The results showed that the different duration and temperatures of thawing influence motility (P<0,05) and the total motile spermatozoa, while the viability, abnormalities, and concentration had no influence (P>0,05). The motility and the total motile spermatozoa reach the highest results at the tap water temperatures T3 (P<0,05), while the lowest results were in T1 (P<0,05). It can be concluded that the different duration and temperatures of thawing influence motility and total motile spermatozoa. The best quality of Simmental bull frozen semen can be obtained with thawing using tap water at 28â„ƒ temperatures for 45 seconds.

Tingkat Keberhasilan Inseminasi Buatan Menggunakan Semen Cair Menggunakan Media Pengencer Tris Aminomethan Kuning Telur pada Sapi Persilangan Ongole Moch Saifudin; Nurul Isnaini; Aulia Puspita Anugra Yekti; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 19, No 1 (2018): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Artificial insemination (AI) using liquid semen give an alternative for the areas doesn’t exist a liquit nitrogen.The purpose of the research was to know about the succesfull artificial insemination (AI) using liquid semen with Tris Aminometan + egg yolk diluent of ongole crossbreed cattle indicated by Non Return Rate (NRR) and Conception Rate (CR). This research was conducted in sub district Tumpang Malang Regency, East Java on June 2017 until November 2017. The material used in this research were 54 ongole crossbreed cows normal reproduction and estrus condition. The method in this research was experimental using 54 heads of cattle with 27 heads inseminated used frozen semen (T0) and 27 heads inseminated liquid semen (T1). The parameters observed were NRR1, NRR2, and NRR3 and CR. The data showed the first parameter of AI success was 81.48% of NRR1, 81.48% of NRR2, and 81.48% of NRR3 used frozen semen and 96.30% of NRR1, 88.89% of NRR2and 81.48% of NRR3 used liquid semen. While for CR showed result 62.96% for frozen semen and 59.26% for liquid semen. In conclusion, the value of NRR for all treatments still meet the AI success standards. The result of Conception Rate of Frozen semen treatments is 62.96% and the liquid semen is 59.26%.Keyword: frozen semen, liquid semen, Non Return Rate, and Conception Rate.Inseminasi buatan (IB) menggunakan semen cair memberikan alternatif daerah yang tidak ada nitrogen cair. Tujuan dari penelitian ini adalah untuk mengevaluasi keberhasilan IB menggunakan semen cair dengan pengencer Tris Aminomethan kuning telur pada sapi persilangan ongol yang berdasarkan Non Return Rate (NRR) dan Conception Rate (CR). Penelitian ini dilaksanakan dikecamatan Tumpang Kabupaten Malang, Jawa Timur pada bulan Juni 2017 hingga November 2017. Materi yang digunakan dalam penelitian ini adalah 54 ekor sapi betina dari berbagai bangsa yang normal dan dalam kondisi birahi. Metode dalam penelitian ini adalah percobaan yang menggunakan 54 ekor sapi dengan rincian 27 ekor di IB semen beku (P0) dan 27 ekor di IB semen cair (P1). Parameter yang diamati adalah NRR1, NRR2, dan NRR3 dan CR. Data menunjukan keberhasilan IB adalah NRR1 81,48%, NRR2 81,48%, dan NRR3 81,48% untuk semen beku dan NRR1 96,30%, NRR2 88, 89%, dan NRR3 81,48% untuk semen cair. Sedangkan untuk CR menunjukkan hasil 62,96% untuk semen beku dan 59,26% untuk semen cair. Kesimpulannya, nilai NRR untuk semua perlakuan masih memenuhi standar keberhasilan IB. Hasil konsepsi perlakuan semen beku adalah 62,96% dan semen cair 59,26%. Kata kunci : semen beku, semen cair, Non Return Rate dan Conception Rate.

Kualitas Semen Cair Sapi Peranakan Ongole pada Pengencer Tris Aminomethan Kuning Telur tanpa Raffinosa yang Disimpan pada Media yang Berbeda Suhu Deny Sulistyowati; Muhammad Azharil Faris; Aulia Puspita Anugra Yekti; Sri Wahjuningsih; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 19, No 1 (2018): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

The aim of this research was to know the storage media with the best temperature to maintain the quality of Ongole crossbred bull liquid semen diluted with tris aminomethan without raffinose + 20% egg yolk. The material used in this research was semen obtained from Ongole crossbred bull located in Sumber Sekar Laboratory of Faculty of Animal Husbandry Universitas Brawijaya Malang. The method was used in laboratory experimental research with experimental design used 5 treatments with 5 replications, there were M1 (thermos containing ice cubes with temperature 0o C), M2 (refrigerator temperature 3-5o C), M3 (thermos containing ice water with temperature 9o C), M4 (thermos containing water with temperature 25o C), and M5 (empty thermos with temperature 28o C). The variables observed were motility, viability, abnormality, and total motile sperm. Data were analyzed using Randomized Block Design, total motile sperm tested using Pearson's Chi Square with expectation value of 40 million motile sperm/ml. The result of this research showed that the storage media had a significant effect on the quality of Ongole crossbred bull liquid semen of motility, viability, and abnormality. Total motile sperm at the 3rd hour showed significantly different M3 and M4 (P <0.05) lower than the expected value of 40 million motile sperm/ml. The conclusion of the research shows that M1 (flask containing ice cubes with temperature 0o C) was the best storage media to carry liquid semen for artificial insemination application in the field.

Peningkatan Conception Rate dengan Inseminasi Buatan Menggunakan Semen Sexing Double Dosis pada Sapi Persilangan Ongole Aulia Puspita Anugra Yekti; Erin Ayu Octaviani; Kuswati Kuswati; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 20, No 2 (2019): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2019.020.02.6

Tujuan dari penelitian ini adalah untuk mengetahui peningkatan conception rate melalui insmeinasi buatan dengan menggunakan semen sexing double dosis pada sapi persilangan ongole. Dalam penelitian ini 96 ekor sapi induk persilangan ongole yang terdiri atas 64 ekor sapi yang diinseminasi dengan semen beku dan 32 ekor sapi yang diinseminasi dengan semen sexing beku double dosis. Sapi yang digunakan dipilih secara acak dengan berdasarkan beberapa kriteria yaitu bebas dari gangguan reproduksi dan menunjukkan tanda-tanda estrus yang jelas. Tempat deposisi semen adalah 4+ atau cornua utery untuk meningkatkan peluang keberhasilan kebuntingan. Penelitian ini terdiri dari dua perlakuan yaitu inseminasi dengan semen beku non sexing (P1) dan semen sexing beku (P2). Parameter yang diamati adalah Non Return Rate (NRR), Service per Conception (S / C) dan Conception Rate (CR). Hasil penelitian menunjukkan bahwa NRR 1 dan NRR2 pada P1 adalah 90,63% dan 79,69%, sedangkan pada P2 masing-masing adalah 81,25% dan 78,12%. Nilai S / C pada P1 adalah 1,09 sedangkan pada P2 adalah 1,19. Sedangkan, nilai CR pada P1 dan P2 masing-masing adalah 43,75% dan 68,75. Dapat disimpulkan bahwa IB dengan semen sexing double dosis dapat meningkatkan nilai conception rate pada Sapi Persilangan Ongole.

Tampilan Reproduksi Sapi Rambon Betina pada Paritas yang Berbeda Ika Fitri Puspitasari; Nurul Isnaini; Aulia Puspita Anugra Yekti; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 19, No 2 (2018): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Penelitian ini bertujuan untuk mengetahui tampilan reproduksi sapi Rambon betina pada paritas yang berbeda. Variabel dalam penelitian ini adalah nilai Service per Conception, Calving Interval, Conception Rate dan Days Open di Kecamatan Glagah, Kabupaten Banyuwangi. Materi yang digunakan adalah 113 ekor sapi Rambon dipilih secara purposive sampling pada paritas yang berbeda. Data dianalisis dengan analisa deskriptif menggunakan nilai rataan dan standar deviasi pada nilai Service per conception, Calving Interval, Conception Rate, Days Open dan dilanjutkan menggunakan uji Kruskal Wallis. Hasil penelitian ini menunjukkan bahwa nilai Service per Conception pada paritas satu memiliki efisiensi terbaik sebesar 1,1±0,31 kali. Nilai Calving Interval pada paritas tiga memiliki efisiensi terbaik sebesar 374,4±29,02 hari. Nilai Days Open pada paritas tiga memiliki efisiensi terbaik sebesar 96,9±27,93 hari. Nilai Conception Rate pada paritas satu menunjukkan efisiensi terbaik yaitu sebesar 90%. Disimpulkan bahwa tampilan reproduksi sapi Rambon di Kecamatan Glagah Kabupaten Banyuwangi dalam kondisi optimal.

Kualitas Spermatozoa Post Thawing Semen Beku Sperma Y Hasil Sexing Pada Sapi Limousin Ali Mahfud; Nurul Isnaini; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 20, No 1 (2019): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2019.020.01.1

Kualitas spermatozoa pada semen beku sexing sangat penting dalam menunjang keberhasilan inseminasi buatan untuk mendapatkan pedat dengan jenis kelamin sesuai harapan. Tujuan penelitian ini bertujuan untuk mengetahui kualitas semen beku semen beku non sexing dan sexing secara makroskopis dan mikroskopis. Materi dalam penelitian menggunakan starw semen beku non sexing dan sexing dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Metode penelitian ini menggunakan eksperimental dengan percobaan labolatorium (laboratory experiment). Variabel penelitian meliputi keadaan umum meliputi tahun produksi straw, secara makroskopis meliputi pH semen beku dan secara mikroskopis meliputi persentase motilitas, viabilitas, abnormalitas, konsentrasi dan total spermatozoa motil. Data yang diperoleh diolah Microsoft Excel dengan analisis Ragam Analysis of variance (Anova) single factor dianalisis secara deskriptif. Hasil penelitian yaitu semen beku non sexing dan sexing meliputi keadaan umum diproduksi tahun 2012 dan 2018, kualitas secara makroskopis memiliki nilai pH sebesar 6,67 dan 6,60 dan kualitas secara mikroskopis memiliki persentase motilitas sebesar 36,00 % dan 31,40 %, viabilitas sebesar 81,70 % dan 75,89 %, abnormalitas sebesar 6,93 % dan 6,78 %, konsentrasi spermatozoa sebesar 31,67 juta/ mini straw dan 12,125 juta/ mini straw dan total spermatozoa motil sebesar 11,39 juta/mini straw dan 5,10125 juta/mini straw. Kesimpulan penelitian bahwa Kualitas post thawing semen beku non sexing lebih tinggi daripada semen beku sexing menggunakan metode SGDP baik secara makroskopis maupun mikroskopis.

Kualitas Semen dengan Berbagai Formulasi Pengencer Dasar Air Kelapa Hijau Selama Simpan Dingin pada Sapi Madura Aulia Puspita Anugra Yekti; Jois Harsah; Muchamad Luthfi; Muhammad Dikman; Asri Nurul Huda; Kuswati Kuswati; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

ABSTRAKInseminasi Buatan dengan menggunakan semen cair digunakan untuk daerah yang sulit nitrogen cair dan mempunyai kualitas yang lebih baik dari pada semen beku. Tujuan penelitian ini adalah untuk mengetahui kualitas berbagai bahan pengencer dasar air kelapa penyimpanan dingin 2-5°C.Penelitian ini dilaksanakan di Loka Penelitian Sapi Potong Grati, Pasuruan. Semen yang digunakan berasal dari sapi madura sebanyak dua ekor, berumur 5 dan 3 tahun dan berat badan yaitu 397 kg dan 360,5 kg. Sapi madura ditampung seminggu 2 kali dengan motilitas > 70% , sedangkan air kelapa yang digunakan adalah air kelapa hijau yang masih muda. Pengenceran semen cair dibagi menjadi 4 yaitu P0 (CEP-3 + 20% kuning telur) sebagai kontrol, P1 (air kelapa hijau +20% kuning telur), P2 (P1 + 0,4% putih telur + 1% fruktosa) dan P3 (P1 + 0,4% putih telur kuning telur +2% fruktosa). Data dianalisis menggunakan uji Pearson’s Chi Square dan Uji Deskriptif. Hasil penelitian menunjukkan motilitas spermatozoa sesuai standar SNI yaitu motilitas> 40% pada pengencer CEP-3 dapat disimpan selama hari ke-8 (40,50±6,43%) sedangkan pada pengencer dasar air kelapa hijau pada P1, P2 dan P3 tidak menunjukkan perbedaan yang nyata (P>0,05) selama disimpan 6 hari yaitu 40,50±10,12%, 38,00±4,22%, 40,00±8,50%. Abnormalitas dari semua perlakuan menunjukan nilai <20%. Viabilitas didapatkan nilai tertinggi pada perlakuan P0(89,58±2,16%) kemudian P1(89,39±3,79%), P2(88,62±4,59%) dan P3(87,93±4,41%).Kata kunci: CEP-3, semen cair, sapi madura, simpan dingin, air kelapa hijau ABSTRACTArtificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%). Keywords:CEP-3, liquid semen, madura bull, cool storage, green coconut water ABSTRACT Artificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%).

KUALITAS DAN KAPASITASI SPERMATOZOA SAPI BALI, MADURA, DAN PERANAKAN ONGOLE Aulia Puspita Anugra Yekti; Willy Saputra Saputra Tatulus; Dian Ratnawati; Luqman Affandhy; Kuswati Kuswati; Asri Nurul Huda; Trinil Susilawati
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 2 (2018): JITRO, Mei
Publisher : Universitas Halu Oleo

ABSTRAKTujuan penelitian ini adalah untuk mengetahui perbedaan kualitas dan kapasitasi spermatozoa pada sapi potong lokal. Selain itu, penelitian ini juga diharapkan dapat digunakan sebagai standar uji kualitas semen. Bahan yang digunakan dalam penelitian ini adalah dua ekor sapi jantan ongole, sapi bali dan sapi madura. Hasil penelitian menunjukkan bahwa persentase motilitas spermatozoa sapi bali 70,83±2,04%, sapi madura 70,00±0,00% dan sapi PO 71,67±2, 58%. persentase Viabilitas spermatozoa sapi bali adalah 89,39±2,84%, sapi madura 90,60 ± 3,13% dan sapi PO 92,13±2,08%. Persentase abnormalitas spermatozoa sapi bali adalah 3,48±1,09%, sapi madura 2,13±0,86% dan sapi PO 2,86±0,51%. Konsentrasi sperma sapi bali 1126,67±169,08 juta/mL, sapi madura 1076,67±3,94 juta/mL dan sapi PO 1210±160,87 juta/mL. Total spermatozoa motil pada sapi bali adalah 3136,9±653,4 juta/mL, sapi madura 3520,41±357,48 juta dan sapi PO 3653,83±1293,59 juta/mL. Persentase status akrosom spermatozoa adalah 85,72±1,72%, sapi madura 85,35±0,76% dan sapi PO 86,40±1,97%. Data yang didapatkan dalam penelitian ini dianalisis menggunakan rancangan acak lengkap dimana hasil analisa menunjukkan tidak ada perbedaan kualitas yang signifikan (P>0,05). Dapat disimpulkan perbedaan jenis pada sapi lokal tidak berpengaruh pada kualitas dan kapasitas spermatozoa, tetapi sapi PO memiliki persentase yang lebih tinggi dari kualitas dan kapasitasi sperma dibandingkan sapi bali dan sapi madura.Kata kunci : kualitas semen, spermatozoa, kapasitasi, sapi lokalABSTRACTThe purpose of this research was to determine the differences in the quality and capacitation of local beef cattle sperm. In addition, this study was also expected to be used as a standard of semen quality test. The material used in this study were two bulls of crossbreed ongole, bali cattle and madura cattle. The results of this study revealed that percentage of motility sperm bali cattle 70.83 ± 2.04%, madura cattle 70.00 ± 0.00% and PO cattle 71.67 ± 2, 58%. percentage of Viability sperm of bali cattle was 89.39 ± 2.84, madura cattle 90.60 ± 3.13% and PO cattle 92.13 ± 2.08%. Percentage of abnormality sperm bali cattle were 3.48 ± 1.09%, madura cattle 2.13 ± 0.86% and PO cattle 2.86 ± 0.51%. percentage of concentration sperm bali cattle 1126.67 ± 169.08 million / mL, madura cattle 1076.67 ± 73.94 million / mL and PO cattle 1210 ± 160.87 million / mL. percentage of total motile sperm bali was 3136.9 ± 653.4 million / mL, madura cattle 3520.41 ± 357.48 million and PO cattle 3653.83 ± 1293.59 million / mL. percentage of status acrosom sperm is 85.72 ± 1.72%, madura cows 85.35 ± 0.76 and cattle PO 86.40 ± 1.97. Data of this research was analyzed using Block Randomized Design (BRD) which showed not significant differences in quality (P> 0.05). The conclusion based on the observations made in this research, differences in local cattle have no effect on the quality and capacitation of spermatozoa, but cross breed ongole cattle have a higher percentage of quality and capacitation of sperm than bali cattle and madura cattle. Semen of bali cattle, and madura cattle used in this study can be used for artificial insemination.Keywords: semen quality, sperm capacitation, local cattle

Co-Authors Achadiah Rachmawati Adelina Ari Hamiyanti Adelina Ari Hamiyanti Ahmad Budi Purnawan Ahmad Salim Ali mahfud Ali Mahfud Alifian Ibnu Ansori Amir Firdaus Andriawan Soni Hantoro Anisa Ramadhani Ardyah Ramadhina Irsanti Putri Aris Sutomo Aryogi Aryogi Asri Nurul Huda Asri Nurul Huda Aziz, Achmad Fadhli Azna Roudlotul Nur Umamah Budi Hartono Chusnul Hanim Dandy Prasetyo Dandy Ulul Azmi Dedi Muhammad Deny Sulistyowati Dewi Masyithoh Dian Ratnawati Dicky Pamungkas Dwi Susan Setiyani Erin Ayu Octaviani Errico Errico Faizal Andri fakhri alfi annashru Febrianto, Nanang Firlia Safa Gatot Ciptadi Habib Asshidiq Syah Hanief Eko Sulistyo Hermanto Hisyam Ryandhika Kusuma Ika Fitri Puspitasari Ika Nurjannah Irvan Mardi Ismail Hasan, Ismail Jois Harsah Joko Riyanto Khairul Rasyad Kurniaesa, Titah Ulya Kusmartono Kusmartono Kuswati , Kuswati Kuswati Kuswati Kuswati Lukman Affandhy Sunarto Luqman Affandhy M. Nur Ihsan Mad Nasir Shamsudin Mashitah ShikhMaidin Mashudi , Mashudi Mashudi Moch Saifudin Muchamad Luthfi Muhammad Ade Salim Muhammad Agus Tahar Muhammad Azharil Faris Muhammad Bayuardhi Muhammad Dikman Muhammad Helmi Muhammad Nur Ihsan Muhammad Zainul Hanif Muhammad Zainul Hanif Nadya Meyta Andriani Nafis, Fardha Ad Durrun Nanang Febrianto Nanang Febrianto Nanang Febrianto Nareswara Hapsari Ndaru Pranowo Widiasmoro Nisa, Dhinda Choirun Nisa’us Sholikah Nolasco da costa Nursahida Ramadhani Nurul Isnaini Nurul Isnaini Nurul Layla Nuryadi Nuryadi Poespitasari Hazanah Ndaru Priyo Sugeng Winarto Puji Akhiroh Putri Utami Putri Utami Putri Utami Putri Utami Putri Utami, Putri Rahmawati, Nanda Ayu Ravenska Ravenska Rheta Eva Ramadhani Setiawan Rifai Mustofa Rizki Prafitri Rizki Prafitri Safitri, Brilla Ismaya Sitiadira Aulia Aldini Sri Wahjuningsih Sri Wahjuningsih Sulistyo, Hanief Eko Syah, Habib Asshidiq Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilawati Trinil Susilowati Wahjuningsih , Sri Wahyudi, Aditiya Willy Saputra Saputra Tatulus Wiranto Wiranto Yadi Malda

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