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All Journal Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Ilmiah Medicamento Physical Therapy Journal of Indonesia
Amaral, Meriana Barreto
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Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Light physical activity and vitamin D improve sperm quality of male Rattus norvegicus exposed to cigarette smoke Amaral, Meriana Barreto; Satriyasa, Bagus Komang; Yasa, I Wayan Putu Sutirta; Sudarmaja, I Made; Jawi, I Made; Manuaba, Ida Bagus Putra; Linawati, Ni Made; Widiyanti, I Gusti Ayu
Physical Therapy Journal of Indonesia Vol. 6 No. 1 (2025): January-June 2025
Publisher : Universitas Udayana dan Diaspora Taipei Medical University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/ptji.v6i1.261

Abstract

Background: Male infertility is a significant global health issue, with rising prevalence linked to environmental factors such as secondhand smoke, which impairs sperm quality. This study aimed to examine how light physical activity and vitamin D supplementation improved sperm quality in rats exposed to cigarette smoke. Methods: This study used a randomized experimental design with 38 rats, divided into two groups. The control group was exposed to cigarette smoke and received vitamin D at 18 IU/kg body weight (BW) daily. The treatment group was exposed to cigarette smoke, received the same vitamin D dose, and underwent light physical activity. The 35-day research period involved the analysis of evaluated parameters. Differences between these parameters were assessed using an independent t-test, with statistical significance set at a P-value < 0.05. Results: Compared to the control group (p-values< 0.001), the treatment group, receiving light physical activity and vitamin D supplementation, exhibited significantly lower malondialdehyde (MDA) levels (0.96 ± 0.14 nmol/mL), higher vitamin D receptor expression (2.44 ± 0.58 cells/HPF), and increased testosterone levels (4.33 ± 0.76 ng/dL). Sperm quality was also significantly improved in the treatment group, showing higher sperm count (2.24 ± 0.81 × 10⁶/mL), motility (4.20 ± 0.23%), viability (3.07 ± 0.39%), and normal morphology (5.34 ± 0.17%) (p-values< 0.001). Conclusion: Light physical activity and vitamin D supplementation improved sperm quality in cigarette smoke-exposed male rats, correlating with reduced MDA and elevated vitamin D receptor expression and testosterone. Further clinical trials are warranted to explore human applications.
Women's Participation in The Selection of Contraceptive Methods – Knowledge, Attitude and Culture Amaral, Meriana Barreto
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 8, No 3: September 2023
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30604/jika.v8i3.2287

Abstract

Timor Leste is one of the developing countries with one of the main problems faced is the population sector, namely the high rate of population growth. The purpose of this study is for what factors influence the knowledge, attitudes and culture of women to participate in the selection of contraceptive methods at the Formosa Health Center. This research method is carried out descriptively with a quantitative approach. The population of this study was Women of reproductive age who sought family planning services at the Formosa Health Center. The results of this study revealed that most participants or women sought family planning services at the Formosa Health Center, dominated by women from the age group of 20 to 24 years and with a higher level of education. We found that there was little information regarding the type of use and source of information, participants with knowledge and level of education preferred to use artificial methods, especially the most widely used ones were Injections, compared to Pills, IUD and Implant. It is highlighted that the side effects of the injection contraceptive method are dizziness and headache. However, there is no fundamental reason to stop use. It is emphasized that only a quarter of women have the knowledge that the advantage of using contraceptives is only to prevent pregnancy.
Anti-Aging Efficacy of Mangrove Leaf Extract Cream Assessed by SPF and Collagen Density Enhancement: In Vitro and In Vivo Approaches Wardani, I Gusti Agung Ayu Kusuma; Adrianta, Ketut Agus; Udayani, Ni Nyoman Wahyu; Amaral, Meriana Barreto; Suena, Ni Made Dharma Shantini
Jurnal Ilmiah Medicamento Vol 12 No 1 (2026): Jurnal Ilmiah Medicamento (In progress)
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v12i1.13138

Abstract

Background: Excessive ultraviolet (UV) exposure is a major contributor to skin photoaging through oxidative stress, inflammation, and collagen degradation. Mangrove leaf extract (Xylocarpus granatum J. Koenig) contains bioactive compounds with potential photoprotective properties. In this study, the extract was formulated into an oil-in-water (O/W) cream suitable for cosmetic applications.Objective: This study aimed to determine the in vitro sun protection factor (SPF), percentage of erythema transmission (%Te), and percentage of pigmentation transmission (%Tp) of the mangrove leaf extract cream, and to evaluate its effectiveness on UVB-exposed guinea pig skin using collagen density as an anti-aging biomarker.Methods: In vitro assessments of SPF, %Te, and %Tp were performed using UV–Vis spectrophotometry. In vivo evaluation was conducted using 20 guinea pigs to examine the effect of the cream on collagen density through histopathological analysis of UVB-exposed skin.Results: The mangrove leaf extract cream exhibited strong photoprotective activity, with SPF values ranging from 20.68 ± 0.51 to 33.70 ± 0.67, %Te values from 3.13 ± 0.08 to 1.75 ± 0.03, and %Tp values from 4.40 ± 0.12 to 3.31 ± 0.03. Furthermore, the cream significantly increased collagen density (71.77 ± 5.27–81.02 ± 0.96) compared with the positive control (52.16 ± 2.32) (p < 0.05).Conclusions: The mangrove leaf extract cream provides effective protection against UVB radiation, characterized by high-protection SPF, extra-protection %Te, and sunblock-level %Tp. The 5% formulation significantly enhanced collagen density, supporting its potential as a natural anti-aging active ingredient for photoaging prevention through improved UV protection and dermal structure.
Co-Authors Bagus Komang Satriyasa I Made Jawi I Made Sudarmaja I Wayan Putu Sutirta Yasa Ida Bagus Putra Manuaba Ketut Agus Adrianta Ni Made Dharma Shantini Suena Ni Made Linawati Ni Nyoman Wahyu Udayani Wardani, I Gusti Agung Ayu Kusuma Widiyanti, I Gusti Ayu