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Fraksi Heksan dan Fraksi Metanol ekstrak biji Pepaya Muda dapat menghambat Spermatid Mencit Jantan (Mus musculus) Komang Satriyasa, Bagus
Jurnal Kedokteran YARSI Vol 16, No 2 (2008): MEI - AGUSTUS 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.247 KB) | DOI: 10.33476/jky.v16i2.241

Abstract

Hexane fraction of unripe papaya seed extract contains glycosides, alkaloid and triterpenoids, which is assumed to have an anti fertility effect, so it can be used as a male contraception, although the mechanism of action is not yet clear. Research is conducted at Animal Laboratory Unit, Department of Pharmacology Faculty of Medicine University of Udayana, and Laboratorium Patologi Balai Penyidikan dan Pengujian Veterian (BPPV) Wilayah VI Denpasar Direktorat Jenderal Peternakan, Departemen Pertanian. This study used the pre-test and posttest control group design, using 30 male mice of balb C strain, aged 12 weeks, weight 20-22 gram, subsequently grouped by random into 3 groups each consisting of 10 male mice. One control group (P0 = control group) was given double distilled water, and two treatment groups (P1 = treatment group) was given fraction of the hexane extract of young Carica papaya seed 20 mg/20gram/day, (P2 = treatment group) was given fraction of the methanol extract of young Carica papaya seed 20 mg/20 gram/day. After 36 days of treatment, evaluation of the testis, of the male mice was conducted. Data were analysed by normality test of Kolmogorov Smirnov Goodness of Fit, homogeneity test, and Anova test. This study showed that spermatic cells decreased significantly (p < 0,01). It was concluded that hexane fraction and methanol fraction of unripe carica papaya seeds extract could decrease spermatic cells of male mice (mus musculus).
PELATIHAN DAYA TAHAN OTOT BERBEBAN ½ KG 20 REPETISI 3 SET MENINGKATKAN FREKUENSI PUKULAN PENDETA TANGAN KIRI DAN KANAN PADA ANGGOTA PENCAK SILAT PERISAI DIRI RANTING SESETAN Suryawan, IKW.; Pangkahila, J.A.; Satriyasa, B.K.; Adiputra, LMIS. Handari; Griadi, IP. Adiartha; Wirawan, IMA.
Jurnal Pendidikan Kesehatan Rekreasi Vol 5 No 2 (2019): Juni 2019
Publisher : Jurnal Pendidikan Kesehatan Rekreasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.338 KB) | DOI: 10.5281/zenodo.3338702

Abstract

Pencak silat adalah seni beladiri yang membutuhkan konsentrasi tinggi. Komponen biomotorik yang terpenting dikembangkan adalah kekuatan, daya tahan otot, kecepatan waktu reaksi dan koordinasi. Dalam penelitian ini peneliti menerapkan pelatihan daya tahan otot untuk meningkatkan frekuensi pukulan pendeta dengan dua tipe pelatihan yaitu, Kelompok 1 melakukan pelatihan daya tahan otot dengan beban dumbbell seberat ½ kg sebanyak 20 repetisi 3 set dan Kelompok 2 melakukan pelatihan daya tahan otot berbeban ½ sebanyak 15 repetisi 4 set. Secara keseluruhan penelitian ini bertujuan untuk meningkatkan frekuensi pukulan pendeta. Perbedaan rerata frekuensi pendeta sebelum pelatihan pada Kelompok 1 26,22 ± 1,21 x/30 detik dan sesudah pelatihan 32,28 ± 1,03 x/30 detik menunjukkan adanya perbedaan bermakna p = 0,000 (p<0,05). Rerata frekuensi pukulan pendetasebelum pelatihan pada Kelompok 2 adalah sebesar 26,22 ± 1,56x/30 detik dan sesudah pelatihan 29,56 ± 1,23x/30 detik (p<0,05). Perbedaan frekuensi pukulan sesudah pelatihan antara kedua kelompok juga menunjukkan perbedaan yang bermakna p = 0,000 (p<0,05). Disimpulkan bahwa pelatihan daya tahan otot berbeban ½ kg 20 repetisi 3 set lebih meningkatkan dibandingkan pelatihan daya tahan otot berbeban ½ kg 15 repetisi 4 set.
Aqueous Extract of Purple Sweet Potato Tubers Decrease MDA and Increase SOD2 in Kidney of Diabetic Rats Satriyasa, Bagus Komang
BALI MEDICAL JOURNAL Vol 5 No 3 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.934 KB)

Abstract

Diabetes mellitus (DM) is a metabolic disease characterized by increase of blood glucose levels or hyperglycemia. Hyperglycemia results in oxidative stress and increase of AGEs. Oxidative stress and metabolic stress lead to reduction of endogenous antioxidant activity resulted in various organs injury especially kidney. The complication is caused by the formation of advanced glycation end products (AGEs), and free radicals. Increase free radicals that occur in diabetics accompanied with decline in endogenous antioxidant activity, such as superoxide dismutase (SOD) and catalase. One way to overcome the complications due to oxidative stress is through utilize the foods which possess natural antioxidant such as purple sweet potato. It has been proven in animals that purple sweet potato had antioxidant properties to prevent various complications in DM. Balinese purple sweet potato tubers contain high anthocyanin and it had been researched as antioxidant in rats in vivo. The aims of this research are to determine the effect of aqueous extract of purple sweet potato tubers to decrease malondialdehyde (MDA) and increase SOD2 in kidney of diabetic rats. This experimental study was conducted with randomized posttest only control group design. Research subject is 38 diabetic rats that divided into 2 groups, controlled group and treatment group, with 18 diabetic rats in each group. Treatment was given for 60 days to each group. Treatment group (P1) is diabetic rats group that was given a standard diet and ad libitum drinks, and 4CC/day/rat of purple sweet potato tubers aqueous extract for 60 days. Controlled group (P0) is diabetic rats group as control that was given a standard diet and ad libitum drinks only. After 60 days of treatment, evaluation was performed. The results of this research found that mean MDA in kidney of control and treatment group was 8.40?0.17 and 1.47?0.15, respectively. There was a significant decreae of MDA (p
Unripe Papaya (Carica papaya L.) Seed Hexane Fraction Extract Inhibits Male Mice (Mus musculus) Spermatogenesis Stronger Than Unripe Papaya Seed Methanolic Extract Satriyasa, Bagus Komang
BALI MEDICAL JOURNAL Vol 5 No 2 (2016)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Men and women have the same rights and obligations in family planning program. Practically, participation in family planning program is still predominated by women while participation of men is still low. Low participation of men in family planning program is due to very limited choices in male contraceptive method. In recent years, studies have been refocused on investigating traditional plants as antifertility herbal medicine for men. Studies on antifertility effects of unripe papaya seeds have been done. However, similar studies on hexane fraction extract have not yet been done. In this study, unripe seeds were collected from local Balinese papaya (Carica papaya) fruits. Objective: This study was aimed to assess the effect of unripe papaya seeds hexane fraction extract on spermatogenesis and the testosterone level of male mice. Hexane fraction extract of unripe papaya seeds contains glycosides and triterpenoids, which is assumed to have an antifertility property, so it can be used as a male contraceptive, although the mechanism of action remains to be elucidated. Method: This study used pre-test and post-test control group design, using 30 male mice of balb C strain, 12 weeks of age, weighing 20-22 gram, which were randomly grouped into 3 groups, each consisting of 10 male mice. One control group (P0 = control group) was given double-distilled water, and two treatment groups were given hexane fraction extract of unripe Carica papaya seeds 20 mg/20gram/day, and methanolic extract of unripe Carica papaya seeds 20 mg/20 gram/day (P1 and P2, respectively). After 36 days of treatment, evaluation of the testes and blood of the male mice was conducted. Results: Data were analysed by normality test of Kolmogorov-Smirnov Goodness of Fit, homogeneity test, and Anova test. This study showed that spermatogonia A, primary pachytene spermatocytes, spermatid and Sertoli cells were decreased significantly (p < 0,05) but Leydig cells and testosterone levels were not decreased significantly (p > 0,05). Conclusion: It can be concluded that hexane fraction extract of unripe Carica papaya seeds can decrease the mean number of spermatogonia A cells, spermatocyte of primary pachytene spermatocytes, spermatid and Sertoli cells better than methanolic extract of unripe Carica papaya seeds.
THE ADDITION OF CAFFEINE IN EARLE’S BALANCED SALT SOLUTION MEDIA WITH WASHING UP METHOD INCREASE MEMBRANE INTEGRITY AND ACROSOMAL SPERM Satriyasa, Bagus Komang; Mahendra, Agung Nova
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: caffeine, a methylxanthine derivate, appears to inhibit phosphodiesterase, thereby inhibiting the break down of cAMP and increasing its concentration inside cell. This study aims to assess the effect of caffeine addition in Earles’s Balanced Salt Solution (EBSS) on the increase in membrane integrity and acrosome reaction of spermatozoa using swim up method Methods: This study was carried out at the Clinic of Sexology and Andrology, Sanglah Public Hospital at Denpasar Bali-Indonesia. This study was an experimental study using the design of pre and post test paired control group design. The samples were sperm specimens of eighteen infertile couple male or volunteers who were infertile with age ranged between 20-40 years old. The samples were divided into two groups: treatment group (caffeine + EBSS) and control group (EBSS). The data were analysed statistically by normality test (Kolmogorov - Smirnov Goodness of Fit Test), Homogeneity test, and Paired Student’s t test. Results: The results showed that the caffeine addition in EBSS medium could increase significantly (p<0.05). The integrity of the sperm membrane obtained were from 81.30 % to 86.60 % and acrosomal reaction from 82.60% to 89.60% evaluated by hypo-osmotic swelling test (HOS). The conclusion of this study is that addition of caffeine in EBSS medium increases significantly membrane integrity and acrosomal reaction of the human sperm.
FRAKSI HEKSAN EKSTRAK BIJI PEPAYA MUDA DAPAT MENGHAMBAT PROSES SPERMATOGENESIS MENCIT JANTAN LEBIH BESAR DARIPADA FRAKSI METANOL EKSTRAK BIJI PAPAYA MUDA Satriyasa, Bagus Komang
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 2 Mei 2008
Publisher : Udayana University

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Abstract

Fraksi ekstrak heksan mengandung dua golongan zat aktif yang bersifat antifertilitas yaitu golongan steroid dan golongan triterpenoid yang diperkirakan bersifat antifertilitas, walupun mekanisme kerjanya belum jelas. Rancangan penelitian yang digunakan ialah ‘’ Pre-test post-test control group design’’. Penelitian ini memakai 30 ekor mencit jantan strain balb C , umur sekitar 12 minggu dengan berat 20-22 gram, kemudian dikelompokkan secara random menjadi 3 kelompok yang masing-masing terdiri dari 10 ekor. Satu kelompok kontrol (P0 = yang diberikan aquabides), dan dua kelompok perlakuan (P1 = kelompok perlakuan yang diberikan fraksi heksan ekstrak 20 mg/20 gram/hari, P2 = kelompok perlakuan yang diberikan fraksi ekstrak metanol 20 mg/20 gram/hari). Setelah 36 hari perlakuan lalu dilakukan pemeriksaan testis dan darah mencit. Data yang diperoleh dianalisis secara statistik dengan menggunakan uji normalitas Kolmogorov Smirnov Goodnees of Fit test, uji homogenitas, dan uji anova. Didapatkan hasil bahwa fraksi heksan ekstrak maupun metanol dapat menurunkan jumlah sel spermatogonia A, sel spermatosit primer pakhiten, sel spermatid, dan sel Sertoli secara sangat bermakna (p < 0,01), sedangkan jumlah sel Leydig dan kadar hormon testosteron menurun tidak bermakna (p > 0,05). Dari hasil penelitian ini dapat disimpulkan bahwa fraksi heksan ekstrak biji pepaya dapat menurunkan jumlah rata-rata sel spermatogonia A, spermatosit primer pakiten, spermatid, sel Sertoli, sel Leydig dan kadar hormon testosteron lebih besar dari pada fraksi metanol ekstrak biji pepaya muda.
FRACTIONS OF THE HEXANE EXTRACT OF YOUNG CARICA PAPAYA SEEDS CAN INHIBIT SPERMATOGENESIS IN MALE MICE MORE THAN FRACTION OF THE METHANOL EXTRACT OF YOUNG CARICA PAPAYA SEEDS Satriyasa, Bagus Komang
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 1 Maret 2008
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.165 KB)

Abstract

Fraction of hexane extract contains glycosides and triterpenoids, which isassumed to have an anti fertility ingredient, so it can be used as a male contraceptive,although the mechanism of action is not yet clear.This study used the pre-test and post-test control group design, using 30 malemice of balb C strain, aged 12 weeks, weight 20-22 gram, subsequently grouped byrandom into 3 groups each consisting of 10 male mice. One control group (P0 = controlgroup) was given double distilled water, and two treatment groups (P1 = treatment group)was given fraction of the hexane extract of young Carica papaya seed 20 mg/20gram/day,P2 = treatment group) was given fraction of the methanol extract of young Carica papayaseed 20 mg/20 gram/day). After 36 days of treatment, evaluation of the testis and blood,of the male mice was conducted.Data were analysed by normality test of Kolmogorov Smirnov Goodness of Fit,homogeneity test, and Anova test. This study showed that cells of spermatogonia A,primary pakhiten spermatocyte, spermatid, and Sertoli cells, decreased significantly (p <0,05) but Leydig cells and testosterone were not decreased significantly (p > 0,05).It is concluded that fraction of hexane extract of carica papaya seeds can decreasethe mean number of cells spermatogonia A, spermatocyte of primary pakhiten, spermatid,Sertoli, and Leydig cells and the level of testosterone hormone better than fraction ofmethanol extract of young Carica papaya seeds.
PEMBERIAN EKSTRAK ETANOL KUBIS UNGU (Brassica oleraceae L.) MENURUNKAN KADAR MALONDIALDEHID DAN JUMLAH MAKROFAG JARINGAN PARU TIKUS YANG TERPAPAR ASAP ROKOK Ganesha, I Gde Haryo; Linawati, Ni Made; Satriyasa, Bagus Komang
Jurnal Ilmiah Medicamento Vol 6 No 1 (2020): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v6i1.714

Abstract

Asap rokok merupakan polutan bagi manusia dan lingkungan. Asap rokok mengandung senyawa radikal bebas yang akan menyebabkan stres oksidatif. Pada kondisi stres oksidatif, radikal bebas menyebabkan peroksidasi lipid membran sel dan merusak organisasi membran sel ditandai dengan peningkatan kadar MDA dan peningkatan jumlah makrofag pada paru. Penggunaan antioksidan dari tumbuhan seperti kubis ungu yang mengandung senyawa flavonoid menjadi salah satu alternative untuk menetralisir terjadinya peningkatan malondialdehyde (MDA) dan peningkatan jumlah makrofag pada paru. Penelitian ini adalah penelitian eksperimental dengan menggunakan post-test control group design yang menggunakan 36 ekor tikus putih (Rattus norvegicus) jantan, berumur 2-3 bulan, dengan berat badan 200 gram. Hasil penelitian menunjukan bahwa rerata jumlah rerata kadar malondialdehyde (MDA) pada kelompok (P0) adalah 4,575±2,034 dan pada kelompok (P1) adalah 2,451±1,036 (p &lt; 0,001). Sedangkan untuk rerata jumlah makrofag pada kelompok (P0) adalah 4,294±0,619, dan kelompok (P1) adalah 1,839±0,624, (p &lt; 0,000). Hasil ini menunjukan secara bermakna bahwa pemberian ekstrak kubis ungu (Brassica oleraceavar. capitata L.) pada tikus galur wistar (Rattus norvegicus) jantan dapat menurunkan kadar malondialdehyde (MDA) dan dapat menurunkan jumlah makrofag pada paru. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa pemberian ekstrak etanol kubis ungu pada tikus galur wistar jantan dapat menurunkan kadar malondialdehyde (MDA) dan dapat menurunkan jumlah makrofag pada paru. tikus galur wistar jantan yang dipaparkan asap rokok sebanyak 3 batang selama 14 hari.
Steroidogenesis Mechanism, Disruption Factor, Gene Function, and Role in Male Fertility : A Mini Review I Gede Widhiantara; Anak Agung Ayu Putri Permatasari; I Wayan Rosiana; Putu Angga Wiradana; BagusKomang Satriyasa
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16914

Abstract

Male fertility can be defined as the ability of the male reproductive system to impregnate a woman, whileinfertility is the condition of a married couple who have been married for one year or more and have had sexualintercourse regularly or without using contraception but do not have a pregnancy or offspring. About 10% ofmarried couples experience infertility. The main organ of male reproduction is the testes because in the testesthe process of forming spermatozoa and the hormone testosterone occurs. The hormone testosterone plays adirect role in the continuity of spermatogenesis. Testosterone is produced through a series of steroidogenesismechanisms in testicular Leydig cells. Several factors influence the course of steroidogenesis such as Leydigcells, steroidogenesis proteins, related genes to the influence of free radicals. These factors are closelyrelated to diet and lifestyle. This study is important to understand in efforts to prevent infertility in men.
PENINGKATAN SEL OSTEOBLAST MANDIBULA TIKUS WISTAR JANTAN SETELAH PEMBERIAN FERMENTASI TEH KOMBUCHA Sardi, Ni Wayan Arni; Sukrama, I Dewa Made; Satriyasa, Bagus Komang
Interdental: Jurnal Kedokteran Gigi Vol 14 No 2 (2018): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Faculty of Dentistry, Mahasaraswati Denpasar University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v14i2.376

Abstract

Osteoblasts are bone-forming cells to grow and play a role in regulation of bone metabolism, including alveolar bone. Imbalance of osteoblasts number in bone formation may lead loss of bone mass, which is causes osteoporosis. Reactive Oxygen Species (ROS) is an oxidant that take important roles in osteoclast activation, osteoclastogenesis and activation of osteoblasts inhibition. Kombucha tea has antioxidant effects. This study was conducted to determine Kombucha tea fermented on 14 days make the osteoblasts in the mandibular bone of male wistar rat more numerous.This research was a purely experimental method with Randomized Post Test Control Group Design. Subjects consisted of ten of three – month male wistar rats were divided into 2 groups. Control group received aquadest for 30 days, on the other hand the treatment group were treated by 8 ml of Kombucha tea for 30 days. On the 31st day, rats were euthanized for tissue sampling and histological preparation of mandibular bone and subjected for HE staining. The test results based on comparison between the control group and treatment group with independent t-test showed that there were significant differences in osteoblasts number in mandibular bone of wistar rats (p<0.05). Kombucha tea contain epicatechin gallate (ECG) that stimulate osteoblasts differentiation and inhibit induction of Receptor Activator Of Nuclear Factor-κB Ligand (RANKL). Further more, Epigallocatechin gallate (EGCG) and theaflavin (TF) detoxify molecules of ROS and enhance osteoblastogenesis. This study concluded that administration of Kombucha tea fermented on 14 days make osteoblasts in the mandibular bone of wistar rat more numerous.
Co-Authors A.N. Mahendra A.W. Indrayani Adiputra, LMIS. Handari Agung Bagus S. Satyarsa Agung Nova Mahendra Agung Wiwiek Indrayani Ali Imron Amaral, Meriana Barreto Anak Agung Gede Eka Septian Utama Anak Agung Gede Fandhiananta Widyanjaya Artini , I Gusti Ayu Ayu Diandra Sari Bastianus Alfian Juatmadja Desak Ketut Ernawati Desak Made Wihandani Dewa Ayu Putu Rasmika Dewi Dewa Putu Gede Purwa Samatra Dita Permatasari Edelweishia, Melissa Efraldo Yudistira Fendy Nugroho Griadi, IP. Adiartha I Dewa Ayu Inten Dwi Primayanti I Dewa Made Sukrama I Dewa Putu Sutjana I Gde Haryo Ganesha I Gede Krisna Putra Yasa I Gede Restu Mahendra Sugiarta I Gede Widhiantara I Gusti Ayu Widianti I Gusti Made Aman I Kadek Arya Gangga Permana I Made Ady Wirawan I Made Jawi I Made Krisna Dinata I MADE MULIARTA . I Made Sudarmaja I Nengah Sandi I Nyoman Adi Putra I Nyoman Mangku Karmaya I Pande Putu Deny Heriwijaya I Putu Adiartha Griadhi I Putu Agus Budi Sudarsana I Putu Eka Kusuma Yasa I Putu Gede Adiatmika I Putu Riska Ardinata I Wayan Putu Sutirta Yasa I Wayan Rosiana I Wayan Sumardika I Wayan Sumardika I Wayan Weta I.A.A. Widhiartini I.G. Wiranatha I.G.A. Artini I.G.M. Aman I.G.M.G.S.C. Trapika IB Putra Manuaba, IB Putra Ida Ayu Mas Suryani Ida Bagus Budha Dharma Kusuma Ida Bagus Ngurah Ida Bagus Putra Manuaba IKW. Suryawan Imakulata Magi Loda Iman Santoso IP. Adiartha Griadi Irawan, Angeline Aprilia Ivana, Jocelyn J. A. Pangkahila Joppi Huwae Karta Sawenda Ketut Agus Adrianta Ketut Tirtayasa Klaudius H. M. Meti Kristanto, Andre Kurniawan, Yukhi Listya Triandari LMIS. Handari Adiputra Lorena Samanta Adelina Luh Made Indah Sri Handari Adiputra Luh Sonya Astana Putri M Widnyana M. Ali Imron Made Indra Prasetya Permana Maura Marda Mayang Sari Muh. Ali Imron Muh. Ali Imron Muh. Irfan Muhammad Irfan Muthiah Munawaroh N. Adiputra N. Adiputra N.W. Tianing N.W.S. Dewi Nabasa, Gayus Erino Ni Luh Gede Ayu Candranita Dharmadi Ni Luh Suriani Ni Made Intansari Tri Buana Ni Made Linawati Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Nyoman Budiani, Ni Nyoman Ni Putu Oka Mahayani* Ni Putu Widya Astuti Ni Putu Wirantari Ni Wayan Arni Sardi Ni Wayan Saka Rahayu Ni Wayan Sinta Devi Arini Ni Wayan Sucindra Dewi Ni Wayan Sucindra Dewi Nyoman Ratep Nyoman Suarjana Ocktaviana Saputri, Legis Ode Mahesa Putra Oktovianus Fufu Oktovianus Fufu Oktovianus Fufu Permatasari, Anak Agung Ayu Putri Putri Uli Saktina Putu Ananda Sutiksna Mulya Putu Angga Wiradana Putu Astawa Putu Susilawati Putu Wandari Pranitha S.Indra Lesmana Selviani, Iit Sugijanto - Sugijanto - Sugijanto - Sugijanto - Suryawan, IKW. Susy Purnawati Syahmirza Indra Lesmana Tharsani Devi Srinivasagam Tjokorda Gde Bagus Mahadewa Tri Wahyu Wulandari Wahyuddin, Wahyuddin Widianti, I Gusti Ayu Widiyanti, I Gusti Ayu Wijaya Surya, I Gede Ngurah Harry Wimpie I Pangkahila Wirawan, IMA. Xena Laveda Yogie Wiswadewa Yuniarti N