Article Per Year (5 Year)
p-Index From 2021 - 2026
0.408
P-Index
This Author published in this journals
All Journal Veterinary Biomedical and Clinical Journal
Pinasti, Roro Titah
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Development of a Specific rsmG Gene Detection Using In House Primer Design of Mycobacterium fortuitum and Modelling Structure of 3D Protein Pinasti, Roro Titah; Kurniawati , Siti; Makta , Averoes Gibraltar; Ramadhani , Najma; Savitry , Diaz Ayu
Veterinary Biomedical and Clinical Journal Vol. 7 No. 1 (2025): Vol. 7 No. 1 2025
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2025.007.01.1

Abstract

Developing specific detection methods for the rsmG gene in Mycobacterium fortuitum is crucial for improving treatment strategies. This study aimed to design and validate an in-house primer detection method for thersmG gene in Mycobacterium fortuitum. To detect the rsmG gene inMycobacterium fortuitum using DNA fragments based on the effectiveness of the in-house primer. Developing this in-house detection method also highlights the importance of local and in-house primer design for specific gene detection using bioinformatics design and structure. This approach can be applied to other genes and bacteria for further research and treatment. This method is used withbioinformatics design from the NCBI database based on the OF855_RS30840and reference sequence NZ_CP107719.1 with gene description of 16S rRNA (guanine (527) N(7) -methyltransferase. The protein model structure template is A0A2U9Q1N61. A using the SWISS-MODEL. The results have 50 - 60% of GC contents from 10 primer pairs with the melting temperature range are 59,33 - 69,46 from NCBI, have forward and reverse. The optimum pairs are the fourth pairs of primers which has optimum self complementarity and relatedness ofeach pair. The results of building model 3D protein were successfully built fromOF855_RS30840. In our conclusion, the primer was obtained and successfullyused to model the 3D structure of the protein encoded by the rsmG gene.
DEVELOPMENT OF BIOINFORMATICS BASED DETECTION AND BUILDING MODEL OF THE MSPA GENE IN MYCOLICIBACTERIUM FORTUITUM. Ramadhani, Najma; Kurniawati, Siti; Pinasti, Roro Titah; Savytri, Diaz Ayu; Makta, Averoes Gibraltar
Veterinary Biomedical and Clinical Journal Vol. 7 No. 2 (2025): Vol. 7 No. 2 2025
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2025.007.02.1

Abstract

The mspA gene is a nanopore protein located in the outer membrane of Mycolicibacterium fortuitum, which plays a role in the uptake pathway for hydrophilic nutrient molecules. The MspA gene is extremely stable against denaturation, making it capable for DNA sequencing. The accuracy of DNA sequencing is highly dependent on the selection of primer pairs. This study aims to develop primers and amplification for the mspA gene in Mycolicibacterium fortuitum. These organisms are typically free-living and can be found widely throughout the environment. Mycolicibacterium fortuitum is responsible for infections in both animals and humans, so there must be a specific detection method. One specific detection method that can be applied is Polymerase Chain Reaction (PCR). An important factor in the PCR technique is the presence of specific primers that will be used in the detection process. The method used involves a bioinformatics approach through the NCBI Primer Blast Program on Mycolicibacterium fortuitum strain MF GZ001 with the template strain reference NZ_CP107719.1, and a structural model approach through protein modelling using the template uun.1.A MSPA. The result of our study was successfully generated from comparing ten primers, only three primers were chosen based on the specific criteria. We also found the successful building for 3D modeling protein from 215 amino acids. In conclusion, the primer development for mspA gene detection resulted in a pair of primer and the 3D structure of the protein encoded by the gene.
Co-Authors Kurniawati , Siti Makta , Averoes Gibraltar Makta, Averoes Gibraltar Ramadhani , Najma Ramadhani, Najma Savitry , Diaz Ayu Savytri, Diaz Ayu Siti Kurniawati