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OPTIMASI SUHU ANNEALING PRIMER DEGENERATE UNTUK MENGAMPLIFIKASI FRAGMEN GEN ARGININE DECARBOXYLASE (ADC) GENOM UBI KAYU LOKAL MALUKU TENGGARA Kurniawati, Siti; Hartati, N. Sri
Indonesian Journal of Biotechnology and Biodiversity Vol 1, No 2 (2017): Indonesian Journal of Biotechnology and Biodiversity
Publisher : Universitas Esa Unggul

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Abstract

AbstractArginine decarboxylase (ADC) is an enzyme that plays a role in polyamine biosynthesis and has been shown to increase resistance to biotic and abiotic stress. Woody oak (Manihot esculenta Crantz) is known to grow and produce well in dry and poor nutrient conditions. The purpose of this study was to obtain optimum conditions in PCR reaction process to obtain candidate gene fragment of ADC. Four pairs of primers to amplify the gene fragments of ADC are degenerate from several plants that have been deposited on NCBI databases, namely Jatropa curcas (Acc XM_022220421), Populus trichocarpa (Acc XM_002306105.2), Capsicum annuum cv Nockwang (Acc KC160547.1) and Lycopersicon esculentum (Acc L16582.1). The success in amplifying a gene by PCR technique using a specially designed primer is determined by the precision of the primary attachment temperature with the DNA mold. Four primer pairs are designed to successfully amplify DNA sequence fragments from the local cassava genome from Malra, namely Malra012 and Malra016 genotypes. The MeadC1 primary pair can amplify the DNA mold and produce bands of less than 1,000 base pairs at a fixed temperature of 46 ° C.47 ° C. and 48 ° C. Nucleotide base sequence analysis using primary pair MeadC1 has been done, but based on bioinformatic analysis using NCBI BLAST program, the obtained fragment did not show the encoding fragment of ADC gene. Keywords : cassava, arginine decarboxylase, AADC AbstrakArginine decarboxylase (ADC) merupakan enzim yang berperan dalan biosintesis poliamin dan telah terbukti dapat meningkatkan ketahanan terhadap cekaman biotik dan abiotik.Ubi kayu (Manihot esculenta Crantz) dikenal mampu tumbuh dan berproduksi dengan baik meski pada kondisi kering dan miskin hara. Tujuan dari penelitian ini adalah untuk mendapatkan kondisi optimum pada proses reaksi PCR untuk memperoleh kandidat fragmen gen ADC. Empat pasang primer untuk mengamplifikasi fragmen gen ADC dirancang secara degenerate dari beberapa tanaman yang telah terdeposit pada database NCBI yaitu Jatropa curcas (Acc XM_012229042.1), Populus trichocarpa (Acc XM_002306105.2), Capsicum annuum cv Nockwang (Acc KC160547.1) dan Lycopersicon esculentum (Acc L16582.1). Keberhasilan dalam amplifikasi suatu gen dengan teknik PCR menggunakan primer yang dirancang khusus sangat ditentukan oleh ketepatan suhu penempelan primer dengan cetakan DNA. Empat pasang primer yang didesain berhasil mengampifikasi fragmen sekuen DNA dari genome ubi kayu lokal asal Malra yaitu genotipe Malra012 dan Malra016. Pasangan primer MeADC1 dapat mengampifikasi cetakan DNA dan menghasilkan pita dengan ukuran kurang dari 1.000 pasang basa pada suhu penempelan 46°C.47°C dan 48°C. Analisis sekuen basa nukleotida menggunakan pasangan primer MeADC1 telah dilakukan, namun berdasarkan analisis bioinformatik menggunakan program BLAST NCBI, fragmen yang diperoleh tidak menunjukkan fragmen penyandi gen ADC. Kata kunci: ubi kayu, arginine decarboxylase, ADC
Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
The Use of Three Species of Lactic Acid Bacteria in the Mocaf (Modified Cassava Flour) Production Seveline, Seveline; Heldyana, Riskya; Kurniawati, Siti
Industria: Jurnal Teknologi dan Manajemen Agroindustri Vol 9, No 3 (2020)
Publisher : Department of Agro-industrial Technology, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.industria.2020.009.03.1

Abstract

AbstractThe use of cassava as foodstuffs has been widely developed. Modifying it into a mocaf (modified cassava flour) is one of the cassava utilization. This research aimed to process cassavas into mocaf utilizing lactic acid bacteria and to obtain a better mocaf in terms of its physicochemical parameters. Bacteria used were L. plantarum, L. fermentum, and L. paracasei, which can ferment cassava to mocaf. The fermentation process was carried out by two fermentation duration of 48 hours and 72 hours, followed by draining and drying using the oven at 50 °C for 6 hours. This research analyzed mocaf's physicochemical properties such as water content, fat content, protein content, ash content, carbohydrate content, whiteness, and acidity. A Factorial Randomized Block Design with two replications was applied as the research design. If the test result showed that the tested sample has a significant difference at the level of significance of 0.05, it then subjects to the further Duncan test, using SPSS. The result showed that the use of L. paracasei produced best characteristics mocaf with a high protein content of 1.44%, an ash content of 0.31%, a white degree of 102.20, and a low degree of acid of 3.66.Keywords: cassava, fermentation duration, L.fermentum, L.paracasei, L.plantarum AbstrakPenggunaan ubi kayu sebagai bahan pangan telah banyak dikembangkan. Salah satu cara dalam pemanfaatan ubi kayu adalah dengan mengolah menjadi produk berupa mocaf (modified cassava flour). Penelitian ini bertujuan untuk mengolah ubi kayu menjadi mocaf dengan bantuan bakteri asam laktat dan mendapatkan hasil tepung mocaf yang lebih baik dari segi fisikokimianya. Bakteri asam laktat yang digunakan adalah L. plantarum, L. fermentum dan L. paracasei yang ternyata memiliki kemampuan dalam memfermentasi ubi kayu menjadi mocaf. Proses fermentasi ubi kayu dilakukan selama dua waktu fermentasi, yaitu 48 jam dan 72 jam yang kemudian dilanjutkan dengan penirisan dan pengeringan dengan oven pada suhu 50 °C selama 6 jam. Analisis dilakukan pada beberapa sifat fisikokimia dari tepung mocaf berupa kadar air, kadar lemak, kadar protein, kadar abu, kadar kabohidrat, derajat putih dan derajat asam. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok Faktorial dengan dua kali ulangan. Jika hasil uji menyatakan bahwa sampel yang diujikan berbeda nyata pada taraf kepercayaan 0,05, maka akan dilakukan uji lanjut Duncan dengan menggunakan SPSS. Hasil yang didapat pada penggunaan L. paracasei menghasilkan tepung mocaf dengan karakteristik terbaik, kadar protein yang tinggi sebesar 1,44%, kadar abu 0,31%, derajat putih 102,20 dan derajat asam yang rendah sebesar 3,66.Kata kunci: L. fermentum, L. paracasei, L. plantarum, lama fermentasi, ubi kayu  
Optimization Of The Annealing Temperature With Degenerate Primer For Amplification Of Arginine Decarboxylase (ADC) Fragment Gene From Genomic DNA of Maluku Tenggara Local Cassava Kurniawati, Siti; Hartati, Sri
Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.669 KB) | DOI: 10.19184/jid.v19i2.6261

Abstract

Arginine decarboxylase (ADC) is a key enzyme responsible for polyamines biosynthesis and has been shown to increase resistance to biotic and abiotic stress. Cassava (Manihot esculenta Crantz.) is able to grow and produce storage roots well on marginal land. The purpose of this study was to optimize annealing temperature of primers in PCR reaction to amplify candidate cassava ADC gene fragments. Annealing temperature is a crucial factor in PCR reaction affecting product (gene fragments) specificity. Four pairs of primers; MeADC1, MeADC2, MeADC3, andMeADC4, were designed using degenerate method from several plants species such as Jatropa curcas (Acc XM_022220421), Populus trichocarpa (Acc XM_002306105.2), Capsicum annuum cv Nockwang (Acc KC160547.1) and Lycopersicon esculentum (Acc L16582.1). All primer pairs successfully amplified DNA fragments from local cassava genotypes (Maluku Tenggara/Malra) including Malra012 and Malra016. The MeADC1 primer amplified DNA fragment with less than 1,000 base pairs (bp) at annealing temperature of 46°C, 47°C and 48°C. However, analysis of PCR product sequencing results using NCBI BLAST method showed that the amplified DNA fragment encodes for ribosomal protein S3 of Oryza minuta (Acc YP_009242005.1).Keywords: arginine decarboxylase, annealing, ADC, cassava, Maluku Tenggara, PCR
The Polymorphic Gene of Single Nucleotide Polymorphism (SNP) of Phytoene Synthase (PSY) to Characterize Carotenoids Yellow Root Cassava Kurniawati, Siti; Hartati, N. Sri; Hartati, Hartati; Sudarmonowati, Enny
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.8 KB) | DOI: 10.19184/jid.v21i1.9197

Abstract

Cassava (Manihot esculenta Crantz.) is a carbohydrate sources containing a limited amount of micronutrients, but some genotypes contain β-carotene as the precursor of vitamin A in the storage roots and leaves. Improvement of β-caroteneand minerals such as Fe / Zn content of cassava’s nutrition is mostly through by biofortification program. The storage root of β-carotene recognized by a yellow or yellowish color while the apical shoots with red to purplish. β-carotenein carotenoid biosynthetic pathway is an expression of the phytoene synthase (PSY) gene. The MePSY2 gene, one of the three MePSY family is the key gene to characterize carotenoids related gene in cassava. In this study, sequencing of the two cassava fulllenght PSY genomic DNA was carried out in conserved areas in the PSY gene region (PSY1 and PSY2) from the DNA of the cassava leaves. Adira1, Carvita25 and Ubi Kuning are yellow root storage genotypes (K1, K2 and K3) while Adira4 and Menti are white root storage genotypes (P1 and P2). Carvita25 is induced somaclonal variant of the Adira4 genotype. Contiq and consensus of nucleotide base sequences from the five cassava genotypes and CM3306-4 cultivars (acc GU111715.1) as references were analysed using the lasergene DNASTAR sequence analysis program. The results of the alignment of the base sequence constituent of the MePSY2 gene showed that the PSY2 gene with amplified genome length was 2,380 base pairs (bp) consisting of 1,140 bp exon region and 1,240 bp intron region. In the conserved coding region, there was a difference of one nucleotide base, that is, base C in two white tuber cassava genotypes replaced with A in three yellow tuber cassava genotypes in the 1.485 base (C1.485A). The SNP converts the amino acid (aa) alanine (A) to aspartic acid (D) at the 191th (A191D). Single Nucleotide polymorphism in conserved coding region can be used further as carotenoid marker for plant breeding of yellow root cassava. Keywords: β carotene, PSY gene, polymorphic gene SNP, yellow root cassava.
Komunikasi, Informasi, dan Edukasi (KIE) Penyakit Hewan Menular Strategis (PHMS) Lumpy Skin Disease kepada Peternak di KUD Kertajaya, Kabupaten Kediri Masardhi, Bagus Aji; Kurniawati, Siti; Dameanti, Fidi Nur Aini Eka Puji
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Lumpy Skin Disease (LSD) merupakan penyakit hewan menular strategis dengan gejala cacar yang dapatmenyebabkan dampak negatif pada kesehatan ternak sapi perah dan ekonomi peternak. LSD disebabkanoleh virus dari genus Capripoxvirus. Virus LSD hanya menyerang pada sapi dan kerbau. Penularan utamaLSD adalah melalui vektor dan kontak langsung antar ternak. Penyakit ini menyebabkan penurunanproduksi susu, infertilitas, dan abortus. Virus LSD dapat menyebabkan kerugian baik dari segi kesehatanternak maupun dari segi ekonomi dan sosial peternak. Salah satu daerah yang memiliki populasi ternakyang relatif besar adalah KUD Kertajaya, Desa Medowo. Peternak KUD Kertajaya Desa Medowomayoritas merupakan peternak tradisional yang mendapatkan informasi terkait LSD sehingga diperlukanadanya KIE dengan tujuan untuk meningkatkan pengetahuan peternak terkait pencegahan dan penangananLSD. Metode KIE yang digunakan adalah menggunakan media informasi (pamflet), presentasi, dan diskusisecara langsung dan penilaian terukur dari hasil pretest, posttest, dan SKM yang dianalisis secara deskriptifdan kuantitatif. Hasil pretest yang diperoleh menunjukkan sebanyak 25% peternak masih belum memilikipemahaman yang komprehensif mengenai LSD.  Hasil posttest diketahui adanya peningkatan sebanyak64% setelah dilakukan KIE. Data SKM menunjukkan adanya respon positif sebesar 88%. Kegiatan KIE inidapat menginisiasi peternak untuk dapat meningkatkan pemahaman terkait LSD pada hewan ternak di KUDKertajaya, Desa Medowo. Kata Kunci: Lumpy Skin Disease, Komunikasi Informasi Edukasi, KUD Kertajaya
Komunikasi, Informasi, dan Edukasi (KIE) Terhadap Penerapan Sanitasi Higiene dalam Upaya Pencegahan Infeksi LSD di Kud Kertajaya Kabupaten Kediri Auranzha, Avriell Jasmine; Kurniawati, Siti; Amri, Indah Amalia
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Lumpy Skin Disease (LSD) merupakan penyakit hewan menular strategis dengan terdapat nodul pada kulityang jelas batasnya, menonjol, dan berdiameter antara 2-5 cm yang dapat menyebabkan dampak negatifpada kesehatan ternak sapi perah dan ekonomi peternak. LSD disebabkan oleh virus Lumpy Skin DiseaseVirus (LSDV) dari genus Capripoxvirus. Virus LSD hanya menyerang pada sapi dan kerbau. Penularanutama LSD adalah melalui vektor dan kontak langsung antar ternak. Penyakit ini menyebabkan penurunanproduksi susu, infertilitas, dan abortus. Virus LSD dapat menyebabkan kerugian baik dari segi kesehatanternak maupun dari segi ekonomi dan sosial peternak. Salah satu daerah yang memiliki populasi ternakyang relatif besar adalah KUD Kertajaya, Desa Medowo. Peternak KUD Kertajaya Desa Medowomayoritas merupakan peternak tradisional yang mendapatkan informasi terkait LSD sehingga diperlukanadanya KIE dengan tujuan untuk meningkatkan pengetahuan peternak terkaitpentingnya sanitasi higienedalam upaya pencegahan infeksi LSD. Metode KIE yang digunakan adalah menggunakan mediainformasi(pamflet), presentasi, dan diskusi secara langsung dan penilaian terukur dari hasil pretest, posttest,dan SKM yang dianalisis secara deskriptif dan kuantitatif. Hasil pretest yang diperoleh menunjukkansebanyak 16,5% peternak masih belum memiliki pemahaman yang komprehensif mengenai pentingnyasanitasi higiene dalam upaya pencegahan infeksi LSD.  Hasil posttest diketahui adanya peningkatansebanyak 12,5% setelah dilakukan KIE. Data SKM menunjukkan adanya respon positif sebesar 88%.Kegiatan KIE ini dapat menginisiasi peternak untuk dapat meningkatkan pemahaman terkait LSD padahewan ternak di KUD Kertajaya, Desa Medowo. Kata Kunci: Lumpy Skin Disease, Sanitasi higiene, Komunikasi Informasi EdukasiAbstract
Komunikasi, Informasi Dan Edukasi Infeksi Penyakit Mulut Dan Kuku Pada Peternak Di KUD Kertajaya Kabupaten Kediri Putri, Salsabila Candra; Kurniawati, Siti; Adrenalin, Sruti Listra
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Penyakit Mulut dan Kuku (PMK) merupakan penyakit yang disebabkan oleh virus Foot and Mouth DiseaseVirus (FMDV) genus Aphtovirus dan famili Picornaviridae. Penyakit Mulut dan Kuku merupakan penyakitmenular dan akut yang menyerang hewan ternak. Hewan ternak yang dapat terserang penyakit PMK adalahhewan berkuku genap atau berkuku belah seperti sapi, kambing, domba, kerbau, dan babi. Penyakit PMKmemiliki tingkat morbiditas mencapai 100% dan tingkat mortalitas sekitar 1.53% dari total kasus yang ada.Kegiatan KIE dilaksanakan di Desa Medowo, yang dikenal sebagai penghasil susu utama dengan sebagianbesar penduduknya bekerja sebagai peternak sapi perah. Kegiatan KIE bertujuan untuk meningkatkanpengetahuan dan pemahaman peternak terkait pencegahan dan penanganan infeksi PMK. Metode KIE yangdigunakan adalah menggunakan media informasi(pamflet), presentasi, diskusi secara langsung danpenilaian terukur dari hasil pretest, posttest, dan SKM yang dianalisis secara deskriptif dan kuantitatif. Hasilanalisis pretest dan posttest menunjukkan peningkatan yang signifikan, pretest menunjukkan terdapat 59%peternak yang memiliki nilai di atas standar. Setelah pelaksanaan kegiatan KIE, hasil posttest menunjukkanadanya peningkatan sebesar 41% dari hasil sebelumnya. Hasil pengisian SKM juga mendukung kegiatanpenyuluhan, peserta mengakui bahwa kegiatan tersebut relevan dan bermanfaat untuk memperluaspengetahuan para peternak. Kegiatan KIE mengenai PMK yang dilakukan kepada peternak di KUDKertajaya terbukti efektif dalam meningkatkan pemahaman peternak terhadap penyakit PMK. Kata Kunci : PMK, Komunikasi Informasi dan Edukasi, Peternak, KUD Kertajaya.
Pengaruh Gaya Kepemimpinan dan Disiplin Kerja terhadap Kinerja Karyawan pada PT Sangwoo Indonesia Kurniawati, Siti; Raharja, Irwan
AKADEMIK: Jurnal Mahasiswa Ekonomi & Bisnis Vol. 5 No. 1 (2025): AKADEMIK: Jurnal Mahasiswa Ekonomi & Bisnis
Publisher : Perhimpunan Sarjana Ekonomi dan Bisnis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37481/jmeb.v5i1.1111

Abstract

Leadership style and work discipline play an important role in improving employee performance. This research aims to examine the influence of leadership style and work discipline on employee performance at PT. Sangwoo Indonesia both partially and simultaneously. This research uses quantitative methods and data collection using questionnaires. Obtain population data on the total number of PT employees. Sangwoo Indonesia as many as 107 and a sample of 84 respondents using the Slovin formula. Data analysts use validity tests, reliability tests, classical assumption tests, hypothesis tests, namely the t test and f test, and partial and simultaneous coefficient of determination tests using SPSS 22. The results of this study show that the t test value on the leadership style variable t count > t table namely 1.794 > 1.663, while for the work discipline variable t count > t table, namely 13.914 > 1.663 so that each variable X has a partial effect on variable Y. Meanwhile, the f test produces a calculated f value > f table, namely 149.154 > 3.109 and a significant value of 0.000 > 0.05 so that Ha is accepted and Ho is rejected, meaning that leadership style and work discipline simultaneously have a significant and positive effect on employee performance. With the results of this research, it is hoped that the leadership of PT. Sangwoo Indonesia can continue to develop its leadership style as well as the employees of PT. Sangwoo Indonesia can continue to improve its work discipline to obtain maximum performance.
Analisis Implementasi Pemanfaatan Lingkungan Sekolah untuk Motivasi Belajar Siswa MI Mata Pelajaran IPA Materi Lingkungan Rosyidah, Af’idatur; Choiriyah, Ma’rifatul; Kurniawati, Siti; Suttrisno, Suttrisno
TARBAWIYAT Vol 3 No 02 (2024): Tarbawiyat: Jurnal Kependidikan
Publisher : Prodi PGMI STAI Al-Akbar Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62589/t.v3i02.342

Abstract

Student motivation to learn is often the main challenge in the learning process, seen from students' lack of attention to the material presented, low active participation, and reluctance to complete assignments. Effective education requires a creative, active, and contextual approach to creating meaningful learning experiences. One potential approach is to utilize the school environment as a learning resource. The school environment, such as parks, gardens, play facilities, and other elements, can be a real and contextual learning medium, allowing students to connect theory with practice. This research aims to analyze the implementation of the use of the school environment in increasing MI students' learning motivation in science subjects, especially environmental material. This research uses a qualitative method with a phenomenological approach, where data is collected through literature studies from various sources such as books, journals and scientific articles. The research results show that the school environment can be used to improve students' cognitive, affective and psychomotor skills, while making learning more enjoyable and effective. Environment-based learning also helps students understand science material, such as healthy and unhealthy environments, more easily. However, there are challenges in its implementation, such as limited school facilities and lack of teacher creativity. This research recommends strategies to maximize the positive impact of using the school environment in supporting student learning motivation holistically.