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All Journal Veterinary Biomedical and Clinical Journal Pena : Jurnal Ilmu Pengetahuan dan Teknologi
Ramadhani , Najma
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Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Veterinary Other

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Development of a Specific rsmG Gene Detection Using In House Primer Design of Mycobacterium fortuitum and Modelling Structure of 3D Protein Pinasti, Roro Titah; Kurniawati , Siti; Makta , Averoes Gibraltar; Ramadhani , Najma; Savitry , Diaz Ayu
Veterinary Biomedical and Clinical Journal Vol. 7 No. 1 (2025): Vol. 7 No. 1 2025
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2025.007.01.1

Abstract

Developing specific detection methods for the rsmG gene in Mycobacterium fortuitum is crucial for improving treatment strategies. This study aimed to design and validate an in-house primer detection method for thersmG gene in Mycobacterium fortuitum. To detect the rsmG gene inMycobacterium fortuitum using DNA fragments based on the effectiveness of the in-house primer. Developing this in-house detection method also highlights the importance of local and in-house primer design for specific gene detection using bioinformatics design and structure. This approach can be applied to other genes and bacteria for further research and treatment. This method is used withbioinformatics design from the NCBI database based on the OF855_RS30840and reference sequence NZ_CP107719.1 with gene description of 16S rRNA (guanine (527) N(7) -methyltransferase. The protein model structure template is A0A2U9Q1N61. A using the SWISS-MODEL. The results have 50 - 60% of GC contents from 10 primer pairs with the melting temperature range are 59,33 - 69,46 from NCBI, have forward and reverse. The optimum pairs are the fourth pairs of primers which has optimum self complementarity and relatedness ofeach pair. The results of building model 3D protein were successfully built fromOF855_RS30840. In our conclusion, the primer was obtained and successfullyused to model the 3D structure of the protein encoded by the rsmG gene.
Deteksi Mycobacteria pada Area Peternakan Unggas di Kabupaten Malang, Jawa Timur: Upaya Pendekatan One Health Kurniawati, Siti; Ramadhani , Najma; Ayu Savitry , Diaz; Titah Pinasti , Roro; Jannah , Miftahul; Ivana Alexsandra Tasumo, Jessica; Gibraltar Makta , Averoes
Pena: Jurnal Ilmu Pengetahuan dan Teknologi Vol. 39 No. 1 (2025): PENA MARET 2025
Publisher : LPPM Universitas Pekalongan

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Abstract

Mycobacteria represent a critical microbial agent with significant implications for animal and human health. Mycobacteria can be found in poultry farm environments, serving as a risk factor for transmission of infection. This study aims to determine the prevalence of Mycobacteria in poultry farming areas. Methods: A total of 25 environmental samples were systematically processed and analyzed using established diagnostic protocols. Mycobacteria identification employed standard microbiological cultivation techniques and acid-fast staining methods. Results The findings revealed an 8% prevalence of Mycobacteria among the examined samples. This prevalence rate demonstrates the bacterial presence within the poultry farming environment, indicating potential transmission risks for both animal and human populations. Conclusion The study successfully documented Mycobacteria presence in poultry farming environments, emphasizing the necessity for continued microbiological surveillance and comprehensive health risk assessments. Further research is recommended to elucidate the specific mechanisms of bacterial transmission and potential mitigation strategies. Keywords: Mycobacteria, Poultry Farm, Soil, Environmental Keywords : consists of, 3-5 terms, in English
Co-Authors Ayu Savitry , Diaz Gibraltar Makta , Averoes Ivana Alexsandra Tasumo, Jessica Jannah , Miftahul Kurniawati , Siti Makta , Averoes Gibraltar Pinasti, Roro Titah Savitry , Diaz Ayu Siti Kurniawati Titah Pinasti , Roro