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Ayu Pratika, Remi
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Chemistry

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Isolation and Characterization of Amylolytic Bacteria From Peatlands Area in Central Kalimantan Nafisah, Zahrotun; Riana Dewi, Syahrani; Ayu Andhita, Naswa; Elya Resha, Enjel; Oksal, Efriyana; Ayu Pratika, Remi; Hermayantiningsih, Dwi
Al-Kimia Vol 13 No 1 (2025): JUNE
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

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Abstract

Peatlands in Central Kalimantan, Indonesia, are ecologically significant due to their acidic, organic-rich conditions, yet their microbial communities remain underexplored, particularly regarding their enzymatic potential. This study aimed to isolate and characterize amylolytic bacteria from peat soil collected at a depth of 143–150 cm in the LAHG CIMTROP area of Central Kalimantan. A total of 44 bacterial isolates were obtained using serial dilution and the gridding method. Screening for amylase activity identified isolate ENS31 as having the highest activity, shown by a clear halo on starch agar. ENS31 was further subjected to biochemical characterization and enzyme activity assays. The amylase enzyme exhibited optimal activity at pH 7 and 50°C, with a specific activity of 3.5 U/mg, and retained activity up to pH 12 (2 U/mg). These results indicate that ENS31 produces an enzyme stable in alkaline conditions despite originating from an acidic environment, demonstrating potential for industrial applications requiring pH and temperature-tolerant biocatalysts. This study contributes to understanding the functional diversity of peatland microbes and highlights tropical peat ecosystems as promising sources of novel enzymes.  
Co-Authors Ayu Andhita, Naswa Elya Resha, Enjel Hermayantiningsih, Dwi Nafisah, Zahrotun Oksal, Efriyana Riana Dewi, Syahrani