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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Unda, Yunike Kurnia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Analysis of Absorbance Value of Flavonoid Level in Brotowali Stem Extract Using UV-VIS Spectrophotometer Unda, Yunike Kurnia; Wulo, Elisabet Sa; Suparno
Jurnal Penelitian Pendidikan IPA Vol 11 No 6 (2025): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i6.10066

Abstract

Flavonoids are polyphenolic secondary metabolites with various biological activities, including antioxidant and anti-inflammatory effects. This study aimed to determine the total flavonoid content in Tinospora crispa (brotowali) stem extract using the AlCl₃ colorimetric method and UV-Vis spectrophotometry. The brotowali stems were extracted using a decoction method with distilled water as the solvent. The formation of a yellow complex between flavonoids and AlCl₃ was measured at a wavelength of 435 nm using a UV-Vis spectrophotometer. A standard calibration curve was prepared using quercetin as the reference compound. The absorbance values of the sample were compared against the quercetin standard curve to determine the flavonoid concentration, expressed as mg quercetin equivalent (QE) per 100 g of extract. The total flavonoid content in the brotowali extract was found to be approximately 0.40%, indicating a moderate level of flavonoids relative to similar medicinal plants. These results suggest that Tinospora crispa stems may serve as a potential source of natural flavonoids with therapeutic applications.
Co-Authors Suparno Wulo, Elisabet Sa