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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Unda, Yunike Kurnia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

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Analysis of Absorbance Value of Flavonoid Level in Brotowali Stem Extract Using UV-VIS Spectrophotometer Unda, Yunike Kurnia; Wulo, Elisabet Sa; Suparno
Jurnal Penelitian Pendidikan IPA Vol 11 No 6 (2025): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i6.10066

Abstract

Flavonoids are polyphenolic secondary metabolites with various biological activities, including antioxidant and anti-inflammatory effects. This study aimed to determine the total flavonoid content in Tinospora crispa (brotowali) stem extract using the AlCl₃ colorimetric method and UV-Vis spectrophotometry. The brotowali stems were extracted using a decoction method with distilled water as the solvent. The formation of a yellow complex between flavonoids and AlCl₃ was measured at a wavelength of 435 nm using a UV-Vis spectrophotometer. A standard calibration curve was prepared using quercetin as the reference compound. The absorbance values of the sample were compared against the quercetin standard curve to determine the flavonoid concentration, expressed as mg quercetin equivalent (QE) per 100 g of extract. The total flavonoid content in the brotowali extract was found to be approximately 0.40%, indicating a moderate level of flavonoids relative to similar medicinal plants. These results suggest that Tinospora crispa stems may serve as a potential source of natural flavonoids with therapeutic applications.
Identification of Phenolic Compounds Content in Tinospora crispa Stem Decoction by FTIR and UV-Visible Spectrophotometry Wulo, Elisabet Sa; Unda, Yunike Kurnia; Suparno
Jurnal Penelitian Pendidikan IPA Vol 11 No 5 (2025): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i5.10069

Abstract

Phenolics are compounds that have a hydroxyl (OH) group attached to an aromatic ring, and are known for various health benefits such as antioxidant, antimicrobial, and anti-inflammatory properties. This study aims to measure the total phenolic content and characterize the functional groups in Tinospora crispa stem extract using a decoction method, as well as evaluate its potential as a source of bioactive compounds. Extraction was performed by boiling dried Tinospora crispa stems in water. Total phenolic content was quantified using UV-Vis spectrophotometry (λ = 765 nm) via the Folin-Ciocalteu reaction, with a gallic acid calibration curve (R² = 0.998). Functional group analysis was conducted using FTIR (4000–400 cm⁻¹). The extract showed high phenolic content (384.909 mg GAE/g), supported by the identification of characteristic functional groups such as O-H (3255.55 cm⁻¹), C=C (2121.59 cm⁻¹), and C-O (1261.45 cm⁻¹) in the FTIR spectrum. These groups are associated with the presence of bioactive compounds such as phenolics. Therefore, Tinospora crispa has potential as a source of phenolic compounds, one of which can be obtained using the decoction method.
Co-Authors Suparno Wulo, Elisabet Sa