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All Journal Biotika: Jurnal Ilmiah Biologi Journal of Social Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Nurhidayat, Fahmi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Physics Social Sciences

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Identifikasi Bakteri Asam Laktat menggunakan Gen Penanda 16S rRNA dari Teh Hitam Fermentasi Syaputri, Yolani; Nurhidayat, Fahmi
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 1 (2025): BIOTIKA JUNI 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i1.63379

Abstract

Teh Hitam merupakan salah satu jenis minuman fermentasi yang berasal dari tanaman Camellia sinensis. Proses fermentasi pada teh hitam terjadi akibat bantuan mikrobiota bakteri asam laktat. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri asam laktat dari isolat teh hitam. Bakteri asam laktat dari larutan teh hitam diisolasi pada medium MRS dalam kondisi anaerob. Gen 16S rRNA diamplifikasi menggunakan universal primer khusus untuk bakteri asam laktat. Purifikasi dan ekstraksi dilakukan menggunakan Fast GeneTM Gel/PCR Extraction kit. Sekuens DNA digabungkan menggunakan metode ClustalW-allignment pada aplikasi Biodit. Sekuens konsensus kemudian dilakukan penjajaran menggunakan BLAST dan dilakukan pembuatan pohon filogenetik pada aplikasi Mega 11 untuk mengetahui hubungan kekerabatan berdasarkan sekuens sampel dan referensi. Hasil analisis filogenetik menunjukkan bahwa sampel bakteri pada isolat teh hitam merupakan Leuconostoc lactis, Lactoplantibacillus plantarum, dan Lactobacillus pentosus.
Deconstruction of Moral Messages in the Naruto Shippuden Anime Series: The Pain Attack on Konoha Village Episode Through Roland Barthes' Semiotic Analysis Nurhidayat, Fahmi; Nurfalah, Faridah
Journal of Social Science Vol. 5 No. 6 (2024): Journal of Social Science
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jss.v5i6.931

Abstract

The Naruto Shippuden Animated Series is one of the best-selling Japanese animated films in Indonesia, the Naruto animated film is widely watched by children both on television and the internet. However, in the Naruto Shippuden animated film there are many violent scenes and adult characters, on that basis the author conducted this study to explore the moral message in the show. Moreover, the Pain Attacking Konoha Village Episode is the most popular episode in the Naruto Shippuden animated series. This series also features various characters with different backgrounds, providing diverse perspectives on morality and action. In every battle depicted, there are layers of meaning that teach values such as courage, sacrifice, and responsibility. The Pain Attacking Konoha Village Episode in particular shows the internal conflict experienced by the main characters, underlining the complexity of moral decisions. The methodology applied in this analysis is a qualitative approach through Roland Barthes' Semiotic Analysis Technique which is based on the identification of signs and markers in Denotative, Connotative and Mythical meanings. This analysis aims to identify how the moral message is conveyed through symbolism and interaction between characters. The findings of this analysis show a number of scenes that implicitly convey moral messages that teach ethics about what is good and right, moral messages of advice, moral messages that show to seek the truth based on facts, moral messages that invite the audience to respect other people's opinions, and moral messages to solve problems with a cool head. It is important to understand that even though there is violence in the show, the values taught serve to motivate the audience to make wise decisions in real life
BIOACTIVE METABOLITES OF Lactiplantibacillus plantarum ISOLATED FROM Sonchus arvensis AS AN ANTIMICROBIAL AGENT Syaputri, Yolani; Nurhidayat, Fahmi; Wulandari, Indri; Rahayu, Sri Rejeki
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6488

Abstract

Sonchus arvensis is a plant widely found in Southeast Asia and often used in traditional medicine. Lactiplantibacillus plantarum is a bacteria isolated from S. arvensis and categorized as qualified presumption of safety by the European Food Safety Authority and the US Food and Drug Administration. Therefore, this study aimed to determine the bioactive metabolites of Lpb. plantarum as antimicrobial agents. Lactic acid bacteria (LAB) were isolated using the serial dilution method, followed by isolation and amplification of the DNA through a 16S rRNA universal primer. Antimicrobial activity was screened using the well-diffusion method. Plantaricin gene identification was performed using PCR and the determination of lactic acid content was conducted using Spectrophotometric. In addition, the titration method was used to measure and determine the hydrogen peroxide. The results showed that Lpb. plantarum had higher inhibition toward pathogen bacteria than Lc. lactis. Lpb. plantarum had the largest inhibition zone against B. subtillis, followed by S. aureus and E. coli, respectively. It was discovered that Lpb. plantarum precipitated with ammonium sulfate had a greater protein content and antibacterial activity. Furthermore, Lpb. plantarum, which encodes plnA and plnEF, produced lactic acid and hydrogen peroxide at concentrations of 3.0158±0.2774 mg/mL and 0.195±0.04 mg/mL, respectively.
BIOACTIVE METABOLITES OF Lactiplantibacillus plantarum ISOLATED FROM Sonchus arvensis AS AN ANTIMICROBIAL AGENT Syaputri, Yolani; Nurhidayat, Fahmi; Wulandari, Indri; Rahayu, Sri Rejeki
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6488

Abstract

Sonchus arvensis is a plant widely found in Southeast Asia and often used in traditional medicine. Lactiplantibacillus plantarum is a bacteria isolated from S. arvensis and categorized as qualified presumption of safety by the European Food Safety Authority and the US Food and Drug Administration. Therefore, this study aimed to determine the bioactive metabolites of Lpb. plantarum as antimicrobial agents. Lactic acid bacteria (LAB) were isolated using the serial dilution method, followed by isolation and amplification of the DNA through a 16S rRNA universal primer. Antimicrobial activity was screened using the well-diffusion method. Plantaricin gene identification was performed using PCR and the determination of lactic acid content was conducted using Spectrophotometric. In addition, the titration method was used to measure and determine the hydrogen peroxide. The results showed that Lpb. plantarum had higher inhibition toward pathogen bacteria than Lc. lactis. Lpb. plantarum had the largest inhibition zone against B. subtillis, followed by S. aureus and E. coli, respectively. It was discovered that Lpb. plantarum precipitated with ammonium sulfate had a greater protein content and antibacterial activity. Furthermore, Lpb. plantarum, which encodes plnA and plnEF, produced lactic acid and hydrogen peroxide at concentrations of 3.0158±0.2774 mg/mL and 0.195±0.04 mg/mL, respectively.
Co-Authors Indri Wulandari, Indri Nurfalah, Faridah Rahayu, Sri Rejeki Syaputri, Yolani