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UJI KUALITAS UDANG BEKU SECARA BAKTERIOLOGIS DARI UNIT PENGOLAHAN IKAN WILAYAH CIREBON, JAWA BARAT Syaputri, Yolani; Nurkhotimah, Retna; Gunawan, Eko Hendri; Amdhika, Ries; Rahayuningsih, Sri Rejeki; Rossiana, Nia
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 1 (2023): BIOTIKA JUNI 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i1.41589

Udang beku merupakan komoditas penting dari sektor perikanan Indonesia. Nilai ekspor udang beku dari tahun ke tahun selalu mengalami peningkatan khususnya dari Unit Pengolahan Ikan (UPI) Cirebon Jawa Barat. Angka Lempeng Total (ALT) serta bakteri patogen seperti Salmonella spp. dan Escherichia coli merupakan parameter bakteriologis yang dapat digunakan sebagai indikator adanya kontaminasi dan kualitas yang kurang baik pada udang. Penelitian ini bertujuan untuk menguji angka lempeng total serta mendeteksi adanya cemaran bakteri Salmonella spp. dan E.coli pada sampel udang beku. Penelitian bersifat deskriptif eksploratif dengan melakukan penelitian di Laboratorium Stasiun Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Cirebon. Berdasarkan standar baku mutu udang beku SNI 2705 : 2020, jumlah angka lempeng total pada udang beku harus dibawah 5.0 x 105 CFU/g serta harus negatif dari bakteri Salmonella spp. dan E. coli. Berdasarkan hasil pengujian keempat sampel udang beku dari UPI wilayah Cirebon Jawa Barat yaitu pada ke empat titik sampel UPI A, B, C, dan D, jumlah angka lempeng total keempat sampel memenuhi syarat standar baku mutu udang beku. Nilai paling tinggi berasal dari UPI C dan yang paling rendah berasal dari UPI D. Hasil analisis bakteri patogen, keempat sampel udang beku negatif bakteri Salmonella spp. dan E. coli.

Identifikasi Keberadaan Escherichia coli, Staphylococcus sp. dan Pseudomonas sp. pada Perairan Sekebitung-Fasilitas Jalatista Universitas Padjadjaran Fitriani, Weni; Azzahra, Rianti Nur; Bachariputri, Iqlima; Syaputri, Yolani
BIOTIKA Jurnal Ilmiah Biologi Vol 22, No 1 (2024): BIOTIKA JUNI 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v22i1.55052

Kebersihan air minum merupakan salah satu komponen penting dalam menjaga kesehatan masyarakat sekitar. Akses universal air bersih dan sanitasi tercantum dalam salah satu poin tujuan pembangunan berkelanjutan pada sektor lingkungan hidup. Secara mikrobiologis, air layak minum harus mengandung 0 coliform per 100 mL air dan tidak mengandung bakteri patogen lainnya. Penelitian ini bertujuan untuk mendeteksi keberadaan bakteri coliform serta bakteri Staphylococcus sp. dan Pseudomonas sp. pada Perairan Sekebitung dan sembilan titik Jalatista. Proses penelitian akan dilakukan dalam enam tahap, mencakup pengambilan sampel, pengukuran pH, isolasi bakteri coliform, isolasi bakteri Staphylococcus sp. dan Pseudomonas sp., perhitungan TPC, pewarnaan Gram, uji IMViC, uji Katalase, dan uji gula-gula. Hasil penelitian menunjukkan pH air pada ke Sembilan titik adalah 7. Jumlah koloni bakteri terbanyak berasal dari sampel Air Siap Minum (Jalatista) di FMIPA Unpad yang ditumbuhkan pada medium MSA, EMB, dan CETA, yaitu 4 x 104 CFU/mL, 3 x 104 CFU/mL dan 1,3 x 103 CFU/mL, berturut-turut. Jumlah koloni bakteri pada EMB Jalatista Rektorat Belakang dan MSA Rektorat Belakang masing-masing sebanyak 1x102 CFU/mL. Hal yang sama ditemukan pada jumlah koloni bakteri dari sampel air Sekebitung, Jalatista Gor Jati Unpad, dan Jalatista FH Unpad yaitu sebanyak 1 x 102CFU/mL. Hasil penelitian lainnya tidak menunjukkan adanya bakteri Escherichia coli, dan Staphylococcus sp. pada sampel air yang diuji. Melainkan, terdapat suspek bakteri Acinetor sp., Bacillus sp., Micrococcus sp., serta Enterobacter sp. yang berhasil terisolasi dan teridentifikasi dengan menggunakan uji IMViC, uji Katalase, dan uji gula-gula.

Antibacterial Activities of Red Mangrove (Rhizophora stylosa Griff.) Leaf Extract against Klebsiella pneumoniae ATCC 700603 Kalasuba, Karina; Rahayuningsih, Sri Rejeki; Amalia, Eri; Syaputri, Yolani; Doni, Febri; Rustama, Mia Miranti
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.661-669

Multidrug-resistant (MDR) Klebsiella pneumoniaeis a critical pathogen causing severe human diseases, including pneumonia. Combating the growing threat of MDRK. pneumoniae requires innovative approaches, such as exploring plant-derived antibacterial agents. Rhizophora stylosa Griff., a mangrove species with traditional medicinal uses, is recognized for its bioactive compounds with potential antibacterial properties. However, research on its bioactive constituents remains limited. This study investigated the antibacterial activity of R. stylosa leaf extracts prepared via maceration and liquid-liquid fractionation against K. pneumoniae ATCC 700603. Using agar-well diffusion and cell leakage assays, the water fraction demonstrated moderate inhibition of K. pneumoniae, producing an average inhibition zone of 8.24 mm and a minimum inhibitory concentration (MIC) of 400 mg/ml. UV-Vis spectrophotometry revealed that the water fraction disrupted protein and nucleic acid synthesis, evidenced by leakage of cellular materials at 260 and 280 nm. Additionally, scanning electron microscopy (SEM) images of K. pneumoniae cells treated with the water fraction showed pore formation and structural damage. These results emphasize the antibacterial potential of the water fraction of R. stylosa leaves against MDRK. pneumoniae. Further investigations are necessary to isolate and identify the specific bioactive compounds responsible for these effects. Moreover, comprehensive assessments of activity and toxicity are crucial to advance R. stylosa-based antibacterial agents as promising alternatives for addressing the challenge of MDR bacterial infections.

Exploration of pathogenic bacteria from lettuce (Lactuca sativa) in a Canteen at Universitas Padjadjaran Chan, Rosamond; Syaputri, Yolani; Syamsuri, Rizky Riscahya Pratama; Rossiana, Nia
Riset Informasi Kesehatan Vol 13 No 2 (2024): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v13i2.872

Background: Lettuce (Lactuca sativa) is commonly consumed raw as a salad, making it highly susceptible to contamination by pathogenic bacteria. This study aims to identify the presence of pathogenic bacteria on raw lettuce to offer an alternative method for handling these pathogens and improving public health. Method: In this research, lettuce samples were bacteriologically tested to identify the presence of pathogenic bacteria. Isolation was performed using selective media, Salmonella-Shigella (SS) agar and Eosin Methylene Blue (EMB) agar, to obtain pathogenic bacteria. Identification was conducted through macroscopic and microscopic examinations, as well as biochemical tests, including indole, Methyl Red, Voges-Proskauer, citrate, motility, and catalase tests. Results: The results of the study revealed the presence of four bacterial isolates: SS 1, SS 2, EMB 1, and EMB 2. Based on the study and identification using "Microbiology: A Laboratory Manual 12th Edition," isolates SS 1, EMB 1, and EMB 2 were identified as Alcaligenes sp., while isolate SS 2 was identified as Proteus sp. Conclusion: These findings provide essential knowledge for managing pathogenic bacterial contamination on lettuce, potentially enhancing food safety and public health.

Identifikasi Bakteri Asam Laktat menggunakan Gen Penanda 16S rRNA dari Teh Hitam Fermentasi Syaputri, Yolani; Nurhidayat, Fahmi
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 1 (2025): BIOTIKA JUNI 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i1.63379

Teh Hitam merupakan salah satu jenis minuman fermentasi yang berasal dari tanaman Camellia sinensis. Proses fermentasi pada teh hitam terjadi akibat bantuan mikrobiota bakteri asam laktat. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri asam laktat dari isolat teh hitam. Bakteri asam laktat dari larutan teh hitam diisolasi pada medium MRS dalam kondisi anaerob. Gen 16S rRNA diamplifikasi menggunakan universal primer khusus untuk bakteri asam laktat. Purifikasi dan ekstraksi dilakukan menggunakan Fast GeneTM Gel/PCR Extraction kit. Sekuens DNA digabungkan menggunakan metode ClustalW-allignment pada aplikasi Biodit. Sekuens konsensus kemudian dilakukan penjajaran menggunakan BLAST dan dilakukan pembuatan pohon filogenetik pada aplikasi Mega 11 untuk mengetahui hubungan kekerabatan berdasarkan sekuens sampel dan referensi. Hasil analisis filogenetik menunjukkan bahwa sampel bakteri pada isolat teh hitam merupakan Leuconostoc lactis, Lactoplantibacillus plantarum, dan Lactobacillus pentosus.

BIOACTIVE METABOLITES OF Lactiplantibacillus plantarum ISOLATED FROM Sonchus arvensis AS AN ANTIMICROBIAL AGENT Syaputri, Yolani; Nurhidayat, Fahmi; Wulandari, Indri; Rahayu, Sri Rejeki
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6488

Sonchus arvensis is a plant widely found in Southeast Asia and often used in traditional medicine. Lactiplantibacillus plantarum is a bacteria isolated from S. arvensis and categorized as qualified presumption of safety by the European Food Safety Authority and the US Food and Drug Administration. Therefore, this study aimed to determine the bioactive metabolites of Lpb. plantarum as antimicrobial agents. Lactic acid bacteria (LAB) were isolated using the serial dilution method, followed by isolation and amplification of the DNA through a 16S rRNA universal primer. Antimicrobial activity was screened using the well-diffusion method. Plantaricin gene identification was performed using PCR and the determination of lactic acid content was conducted using Spectrophotometric. In addition, the titration method was used to measure and determine the hydrogen peroxide. The results showed that Lpb. plantarum had higher inhibition toward pathogen bacteria than Lc. lactis. Lpb. plantarum had the largest inhibition zone against B. subtillis, followed by S. aureus and E. coli, respectively. It was discovered that Lpb. plantarum precipitated with ammonium sulfate had a greater protein content and antibacterial activity. Furthermore, Lpb. plantarum, which encodes plnA and plnEF, produced lactic acid and hydrogen peroxide at concentrations of 3.0158±0.2774 mg/mL and 0.195±0.04 mg/mL, respectively.

BACTERIAL CONTAMINATION TEST IN POWDER-FORMULATED Helicoverpa armige-ra NUCLEAR POLYHEDROSIS VIRUS (HaNPV1) SUBCULTURE Miranti, Mia; Kasmara, Hikmat; Fitriani, Nurullia; Melanie, Melanie; A'yun, Inas Qurrata; Syaputri, Yolani; Doni, Febri; Madihah, Madihah; Rahayuningsih, Sri Rejeki; Azizah, Nabilah Sekar; Hermawan, Wawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2947

The Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV1) is a subculture derived from the original HaNPV, and it has been cultivated in Spodoptera litura larvae as an alternative host. HaNPV1 was subsequently formulated using gypsum and talcum as carrier media. Following this formulation, a bacterial contamination test was conducted to assess the quality of the viral formulation. The experiment was arranged in the randomized factorial block design (RFBD) with 2 replications. The viral formulations was stored for 16 weeks and the samples were taken every two weeks for contamination analysis. The data was then analyzed with the analysis of variance (ANOVA) and a post-hoc using Duncan’s Multiple Range test. The variable observed was the number of the bacterial colonies cultivated on the specific media i.e., Nutrient Agar (NA), Salmonella Shigella Agar (SSA) and Eosin Methilen Blue Agar (EMB). The results showed that the bacterial contaminants was detected from 0 to 12 weeks of storage time. However, the highest contamination was found in viral formulation after 8 weeks of storage time and the highest bacterial contaminations were recorded from all viral formulation tested in NA. The results indicated that the bacterial contamination were found around 1.45 × 109 cfu/gram and 1.97 × 109 cfu/gram in gypsum and talcum formulations, respectively. On SSA and EMB media, the bacteria contaminants from all formulation found in 8 weeks of storage time, but Salmonella, Shigella, or Escherichia coli (aspathogenic bacteria) were not found. After 12 weeks storage time, there was no indication of contamination found in all media. Furthermore, Bacillus species was found as a most dominant contaminant in all samples. In conclusion, although the viral formulations using gypsum and talc were not contaminated by pathogenic bacteria such Salmonella, Shigella, or E. coli. Nevetherless, the viral formulation was still easily contaminated by other non-pathogenic bacterial species. Thus, a more standardized and stricted strategy needs to be developed for a better viral formulation product.

BIOACTIVE METABOLITES OF Lactiplantibacillus plantarum ISOLATED FROM Sonchus arvensis AS AN ANTIMICROBIAL AGENT Syaputri, Yolani; Nurhidayat, Fahmi; Wulandari, Indri; Rahayu, Sri Rejeki
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6488

Sonchus arvensis is a plant widely found in Southeast Asia and often used in traditional medicine. Lactiplantibacillus plantarum is a bacteria isolated from S. arvensis and categorized as qualified presumption of safety by the European Food Safety Authority and the US Food and Drug Administration. Therefore, this study aimed to determine the bioactive metabolites of Lpb. plantarum as antimicrobial agents. Lactic acid bacteria (LAB) were isolated using the serial dilution method, followed by isolation and amplification of the DNA through a 16S rRNA universal primer. Antimicrobial activity was screened using the well-diffusion method. Plantaricin gene identification was performed using PCR and the determination of lactic acid content was conducted using Spectrophotometric. In addition, the titration method was used to measure and determine the hydrogen peroxide. The results showed that Lpb. plantarum had higher inhibition toward pathogen bacteria than Lc. lactis. Lpb. plantarum had the largest inhibition zone against B. subtillis, followed by S. aureus and E. coli, respectively. It was discovered that Lpb. plantarum precipitated with ammonium sulfate had a greater protein content and antibacterial activity. Furthermore, Lpb. plantarum, which encodes plnA and plnEF, produced lactic acid and hydrogen peroxide at concentrations of 3.0158±0.2774 mg/mL and 0.195±0.04 mg/mL, respectively.

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