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All Journal Journal of Natural Sciences Journal of BioSustainability
Yuliandini, Sinta
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Optimization of Cellulase Enzyme Production from Corn Cob Waste by Cellulolytic Fungus Aspergillus niger Yuliandini, Sinta; Peristiwati, Peristiwati; Surakusumah, Wahyu
Journal of Natural Sciences Vol 6, No 2 (2025): Journal of Natural Sciences Juli
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i2.916

Abstract

Corncob is an agricultural waste rich in cellulose, making it a promising alternative substrate for cellulase enzyme production, which is widely used in various industrial applications such as bioenergy, food, and textile industries. Aspergillus niger is a well-known cellulolytic fungus capable of efficiently producing cellulase enzymes. This study aimed to optimize cellulase production by A. niger using corncob powder (Zea mays) as a substrate through submerged fermentation (SmF), with variations in temperature (29.5°C and 30.5°C) and pH (4.5 and 5.5). The cellulolytic activity of A. niger was tested on CMC agar medium. Cellulase activity was analyzed based on the concentration of reducing sugars using the DNS method. The highest enzymatic activity was recorded at 1.125 IU/mL with a biomass yield of 0.754 mg/mL at 30.5°C and pH 4.5. These findings suggest that optimizing environmental conditions and utilizing corncob waste as a carbon source can enhance cellulase production by A. niger, offering a sustainable and cost-effective approach for enzyme-based industries.
Effect of Exogenous Bacillus sp. Addition on Bioremediation of Soil Contaminated by Used Cooking Oil Fauziah, Aisyah Fikria; Prameswari, Alfi Hanifah; Polandos, Chersy Tiffany; Kocha, Santana; Yuliandini, Sinta; Surakusumah, Wahyu; Dewina, Restu Utari
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

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Abstract

Used cooking oil waste can be carcinogenic and toxic to living creatures in soil. Bioremediation is a way to treat used cooking oil-contaminated soil by using microorganisms that are safe for the environment. This research aimed to determine the effect of adding exogenous bacteria Bacillus sp. on the bioremediation process of soil contaminated with used cooking oil waste. Total Petroleum Hydrocarbons (TPH) measurement and effectiveness of Bacillus sp. in degrading hydrocarbons was calculated in this study. The results showed that Bacillus sp. has the ability to rapidly degrade used cooking oil TPH and increased the effectiveness of bioremediation in soil.
Co-Authors Dewina, Restu Utari Fauziah, Aisyah Fikria Kocha, Santana Peristiwati, Peristiwati Polandos, Chersy Tiffany Prameswari, Alfi Hanifah Wahyu Surakusumah