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All Journal Jurnal SOLMA Journal of Multidisciplinary Applied Natural Science
Anbiya, Lulu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Other

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Pelatihan Analisis Genomik Melalui Pengenalan dan Penggunaan Peralatan di Laboratorium Biomolekuler, Universitas Lampung Mahfut, Mahfut; Wandono, Petrus Tri Aji; Ismail, Ikhwan; Anbiya, Lulu; Arsitalia, Metari; Sari, V. Dwi Anggita; Asadudin, David
Jurnal SOLMA Vol. 14 No. 2 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i2.18363

Abstract

Pendahuluan: Kemajuan biologi molekuler membuka peluang besar dalam riset genomik, berdampak luas pada bidang kesehatan, pertanian, dan lingkungan. Diperlukan peningkatan keterampilan sivitas akademika dalam pengoperasian alat laboratorium biomolekuler. Metode: Pelatihan dilakukan melalui pendekatan teori dan praktik langsung (hands-on training), dengan evaluasi menggunakan pre-test dan post-test untuk mengukur peningkatan pemahaman peserta. Hasil: Terdapat peningkatan signifikan pada skor post-test, terutama dalam penerapan alat untuk riset. Peserta menunjukkan peningkatan pemahaman, khususnya dalam aspek teknis penggunaan alat. Kesimpulan: Pelatihan ini efektif dalam meningkatkan kompetensi laboratorium peserta. Direkomendasikan pelatihan lanjutan dan pendampingan rutin untuk memperkuat keterampilan riset biologi molekuler secara berkelanjutan.
Stomata Characterization of Native Dendrobium in Liwa Botanical Garden Wahyuningsih, Sri; Mahfut, Mahfut; Anbiya, Lulu
Journal of Multidisciplinary Applied Natural Science Vol. 6 No. 2 (2026): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.347

Abstract

Dendrobium is one of the most commonly collected native orchid genera at the Liwa Botanical Garden, Lampung, Indonesia. In its conservation efforts, the identification of native orchid species through anatomical characterization is essential. The aim of this research is to identify the native Dendrobium species collection at the Liwa Botanical Garden based on stomatal anatomical characteristics and to confirm these results with previous morphological and molecular characterization. The research steps involved the collection of leaves from 19 Dendrobium accessions at the Liwa Botanical Garden, while the anatomical characterization was conducted by preparing paradermal sections to microscopically observe the stomata. The main anatomical characteristics observed included stomatal aperture width, stomatal length, stomatal width, number of stomata, stomatal density, and stomatal index. The results of the study show that, overall, the stomatal aperture width is 2.88 μm, stomatal length is 12.38 μm, stomatal width is 12.58 μm, stomatal density is 29 stomata/mm², and stomatal index is 0.061%. Phenetic analysis based on the dendrogram divided the different native Dendrobium samples into two clusters (A and B) with similarity indices of 1.60 and 0.90, and PCA values (0.170 and 0.044) were found to be greater than 0.02, indicating the contribution of each group. The PCA value of 0.170 reflects the influence of stomatal area, whereas 0.044 reflects the influence of stomatal aperture width, stomatal index, and stomatal density. The anatomical characterization results show a correlation with the identification outcomes based on morphological and molecular characteristics. Furthermore, these findings can serve as a recommendation for the identification of native orchid species and provide a basis for the conservation efforts of native Dendrobium at the Liwa Botanical Garden, Indonesia.
Co-Authors Arsitalia, Metari Asadudin, David Ismail, Ikhwan Mahfut Sari, V. Dwi Anggita Wandono, Petrus Tri Aji