Article Per Year (5 Year)
p-Index From 2021 - 2026
1.084
P-Index
This Author published in this journals
All Journal Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA Jurnal Biogenerasi Journal of Science and Education Research
Wirdullutfi, Wirdullutfi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Other

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Menakar Literasi Mikrobiologi Mahasiswa Pendidikan Biologi Universitas Mataram Sakaroni, Rubiyatna; Wirdullutfi, Wirdullutfi; Hijriani, Baiq Isti
Journal of Science and Education Research Vol. 4 No. 2 (2025): Journal of Science and Education Research
Publisher : Yayasan Pendidikan Insan Mulia Utan Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62759/jser.v4i2.316

Abstract

Microbiology literacy is an important skill for prospective biology teachers to be able to understand, teach, and apply microbiology concepts appropriately in life and learning. This study aims to describe the microbiology literacy profile of biology education students at Mataram University based on competency and attitude dimensions after completing microbiology courses. The study employs a quantitative descriptive approach using instruments developed based on the PISA 2015 science literacy framework. The research subjects were students who had taken microbiology courses. Data were analyzed descriptively to determine literacy score categories. The results showed that students' microbiology literacy was generally in the moderate to low category. In the competency dimension, the highest scores were in the ability to interpret data and scientific evidence, while the lowest scores were in the ability to evaluate and design scientific investigations. In the dimension of attitude, the majority of students were in the moderate category, with the highest scores in the assessment of scientific approaches and the lowest scores in interest in microbiology. These low scores indicate the need to strengthen higher-order thinking skills, hands-on experience in research design, and learning that links microbiology concepts to real-life contexts. This study recommends the use of project-based, problem-based, and socio-scientific issue-based learning strategies, as well as evaluations that require the application of concepts and analysis. This approach is expected to enhance students' conceptual understanding, analytical skills, and scientific attitudes in a balanced manner.
ANALYSIS OF ACTINOMYCETES ABUNDANCE IN THE RHIZOSPHERE OF MANGROVE ECOSYSTEMS IN SEKOTONG AS A POTENTIAL SOURCE OF BENEFICIAL MICROBES Sakaroni, Rubiyatna; Baiq Isti Hijriani; Wirdullutfi
Jurnal Biogenerasi Vol. 10 No. 3 (2025): Volume 10 no 3 tahun 2025 terbit september
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i3.6701

Abstract

The rhizosphere of mangroves is a microhabitat rich in root exudates and organic matter, making it an ideal environment for the growth of Actinomycetes. This study aims to analyze the abundance of Actinomycetes in the mangrove rhizosphere at three different locations in Sekotong District, West Lombok: Bagek Kembar, Tanjung Batu, and Buwun Mas. Sampling was conducted using purposive sampling in the dominant root zone at a depth of ±20 cm. Actinomycetes isolation was performed using the surface plate method on SCA medium supplemented with nystatin as an antifungal agent. The results showed that the highest abundance was found in the rhizosphere of Rhizophora mucronata at Bagek Kembar with 4.6 × 10⁵ CFU/g, while the lowest was in Avicennia marina at Tanjung Batu with 1 × 10⁵ CFU/g. In general, Rhizophora mucronata showed higher abundance than Avicennia marina in all locations. This difference was influenced by root exudate characteristics, root system structure, and soil physicochemical conditions such as aeration and organic matter content. This study concludes that the rhizosphere of Rhizophora mucronata in Bagek Kembar is a more supportive habitat for the growth of Actinomycetes. This finding serves as an important basis for further exploration of Actinomycetes isolates from mangroves as potential sources of biocontrol agents and antimicrobial compound producers.
Sosialisasi Deteksi Dini Penyakit Tidak Menular dan Pemeriksaan Kesehatan dengan Metode POCT Wirdullutfi; Baiq Isti HIjriani; Heru Setiawan; Eni Suyantri; Marosa Robi’atul Adawiyah
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.14141

Abstract

This community service program aims to enhance participants' knowledge, awareness, and independence in the early detection of non-communicable diseases (NCDs) through blood pressure, blood glucose, and uric acid screening, as an effort to control NCD cases in line with the 2030 Sustainable Development Goals (SDGs) for global well-being. The methods implemented included socialization and health screenings for 55 participants (average age 41 years; range 26-56 years; 67% male, 33% female), with 50 participants screened for blood glucose and uric acid (74% male, 26% female). Screening results showed: normal blood pressure in 45 people (81.9%), prehypertension in 8 people (14.5%), stage 1 hypertension in 2 people (3.6%); normal blood glucose in 44 people (88%), prediabetes in 5 people (10%), diabetes in 1 person (2%); and normal uric acid levels in 47 people (94%), hyperuricemia in 3 people (6%). All participants understood the importance of early NCD detection and committed to creating a healthier and more productive community.
Effect of Follicular Substances on Phytochemical Profile and Antioxidant Capacity of Ketip Banana (Musa paradisiaca Forma typiaca) Peel Ethanol Extract Bahri, Syamsul; Hijriani, Baiq Isti; Wirdullutfi, Wirdullutfi
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.10868

Abstract

A healthy lifestyles require both synthetic antioxidants and those sourced from food. Antioxidants can be obtained from food ingredients or can also be synthetic oxidants. Antioxidants derived from plants contain quite high levels. One type of tropical fruit contains antioxidants is the banana, and one cultivar of banana is Musa paradisiaca Forma typiaca, locally name is Ketip. The research aimed to compare the phytochemical profile of peel ethanol extract of this cultivar using different sample preparation techniques and then compare the antioxidant capacity of the extracts using the DPPH method. This research employed two types of techniques in preparing the sample. The first one leaves the sap attached to the sample, the second one uses tissue paper to reduce the follicular substances, soon after chopping the sample. Phytochemical results of the extract prepared by the first technique showed that the extract contains flavonoids, alkaloids, saponins, terpenoids, and tannins, while the extract prepared by the second technique also detected phenolic compounds. Despite the phytochemical profile showing no difference at all from both techniques, their antioxidant capacity showed a significant difference. The IC50 score of the extract prepared by the first technique was 554,84. This score is categorized in the very weak activity group, whereas the IC50 score of the extract prepared by the second technique was 202,13. This score is categorized in the intermediate activity group. It is concluded that the follicular substances significantly increase the IC50 score of ethanol extract of Ketip banana peel.
The Effect of Methanolic Extract of Asiatic Bitter Yam (Dioscorea hispida) Tuber on 3rd Instar Larvae Drosophila melanogaster Mortality Bahri, Syamsul; Wirdullutfi, Wirdullutfi
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.11026

Abstract

Drosophila melanogaster is a cosmopolitan insect widely used in genetic studies due to its short life cycle, large offspring numbers, and low cost. One of the plant species known to contain natural pesticides is asiatic bitter yam, which is known by the local name of gadung. Tubers of this species contain dioscorine toxins that have pesticide properties, which can cause nervous disorders. This study aimed to determine the effect of asiatic bitter yam tuber extract (Dioscorea hispida) on 3rd instar larvae of Drosophila melanogaster. The tuber extract was obtained by macerating the sample in 96% alcohol for 72 hours, followed by sieving and then evaporating the extract using a rotatory evaporator. The effect of eight concentrations of tuber extract, namely 0 ppm, 50 ppm, 100 ppm, 150 ppm, 200 ppm, 250 ppm, 300 ppm, and 350 ppm on 3rd instar larvae mortality of this species was studied using a completely randomized design. Every single treatment used 6 larvae with 5 repetitions. The results demonstrated a positive correlation between increasing concentrations of asiatic bitter yam tuber extract and larval mortality rates. One-way ANOVA statistical analysis showed a significant effect of the extract on larval mortality (p < 0.05). Results showed that the asiatic bitter yam tuber extract had a significant effect on 3rd instar larvae Drosophila melanogaster mortality, as the F value was greater than the F critical (10.28571>2.312741). However, the highest percentage of mortality caused by this extract is lower than the value required by the World Food Agency. since the mortality caused by this extract less than 50%, its effect is still below the minimum standard as a pesticide.
Antioxidant Activity of Methanol Extract Leaves of Neem (Azadirachta indica) Bahri, Syamsul; Wirdullutfi, Wirdullutfi; Hijriani, Baiq Isti
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.11048

Abstract

Azadirachta indica Juss. (neem), a medicinal plant belonging to the Meliaceae. The leaves of this species contain bioactive compounds such as flavonoids, alkaloids, steroids, saponins, tannins, and azadirachtin. These compounds exhibit antimicrobial activity against Salmonella and Escherichia coli. This study aimed to determine the antioxidant activity of the neem leaves' methanol extract by using the DPPH method. The research method used phytochemical screening by separating chemical compounds from samples using 70% methanol solvent. The result showed that the antioxidant activity of the neem leaves' methanol extract is classified in the weak category with an IC50 score of 426.15 mg/mL. One of the main factors explaining the high IC50 values is the differences in the chemical structure and molecular shape of the secondary metabolites contained in the extracts. The antioxidant activity of flavonoids and phenolics is greatly influenced by the number and position of hydroxyl groups, the degree of conjugation, and the presence of sugar groups.
Qualitative Phytochemical Screening of Ethanolic Extract of Ocimum basilicum L. Leaves Bahri, Syamsul; Wirdullutfi, Wirdullutfi; Hijriani, Baiq Isti
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.11142

Abstract

Ocimum basilicum L. (basil) leaves are widely recognized for their potential bioactive compounds. This study aimed to qualitatively evaluate the phytochemical constituents of ethanolic extracts of basil leaves. Dried basil leaves were extracted using 96% ethanol, and the resulting extract was subjected to standard phytochemical screening tests to identify major secondary metabolites. The results revealed a strong presence of alkaloids and steroids, indicating that these compounds are dominant constituents in the ethanolic extract. In addition, flavonoids, tannins, and phenolic compounds were detected at lower intensities, suggesting their presence in smaller amounts. Conversely, terpenoids and saponins were not detected in the extract. These findings indicate that ethanol is an effective solvent for extracting alkaloids and steroids from basil leaves, while its efficiency for terpenoids and saponins may be limited. The presence of these secondary metabolites supports the potential biological activity of Ocimum basilicum leaves and provides a scientific basis for further studies on their pharmacological and biological activities.  
Co-Authors BaiqIsti Hijriani Eni Suyantri HERU SETIAWAN Marosa Robi’atul Adawiyah Sakaroni, Rubiyatna Syamsul Bahri