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All Journal Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA Indonesian Journal of Educational Science (IJES) Jurnal Biogenerasi BIOEDUPAT: Pattimura Journal of Biology and Learning Islam Realitas: Journal of Islamic and Social Studies Journal of Science and Education Research
Sakaroni, Rubiyatna
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Other

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Melatih Scientific Explanation Siswa melalui Learning Cycle 5E berkonteks Socio-Scientific Issues (SSI) Ningthias, Dyah Puspitasari; Listantia, Nora; Sakaroni, Rubiyatna
Indonesian Journal of Educational Science (IJES) Vol 7 No 1 (2024): Indonesian Journal of Educational Science (IJES)
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/ijes.v7i1.4562

Abstract

Kemampuan siswa dalam memberikan penjelasan ilmiah (scientific explanation) merupakan salah satu keterampilan abad ke-21 yang penting untuk dikembangkan melalui pembelajaran yang inovatif. Namun, pembelajaran sains di Indonesia masih banyak berfokus pada hafalan konsep tanpa memberikan ruang bagi siswa untuk menghubungkan sains dengan kehidupan nyata. Salah satu pendekatan yang potensial adalah penggunaan model Learning Cycle 5E yang dikontekstualisasikan dengan Socio-Scientific Issues (SSI). Pendekatan ini memungkinkan siswa untuk melakukan penyelidikan berbasis bukti, menggunakan konsep-konsep sains, khususnya kimia, untuk menjelaskan isu-isu yang relevan dengan kehidupan sosial. Penelitian ini bertujuan untuk mengetahui efektivitas penerapan model Learning Cycle 5E berbasis SSI dalam melatih kemampuan scientific explanation siswa. Penelitian melibatkan 33 siswa di kelas eksperimen I, 33 siswa di kelas eksperimen II, dan 34 siswa di kelas kontrol. Keterampilan penjelasan ilmiah siswa dikategorikan menggunakan Taksonomi SOLO (Structure of the Observed Learning Outcome). Hasil investigasi menunjukkan bahwa pembelajaran dengan Learning Cycle 5E berbasis SSI lebih efektif dalam meningkatkan keterampilan scientific explanation dibandingkan model Learning Cycle 5E tanpa konteks SSI maupun pembelajaran konvensional. Novelty dari penelitian ini terletak pada integrasi model Learning Cycle 5E dengan isu sosio-sains sebagai konteks pembelajaran, yang jarang dilakukan di pembelajaran kimia, sehingga dapat memberikan kontribusi baru terhadap praktik pembelajaran berbasis inkuiri dan konteks nyata.
Understanding Contextual Strategies and Engaging Learning from SMA NW Kopang Biology Teacher to Enhance Student Motivation Sakaroni, Rubiyatna; Listantia, Nora; Ningthias, Dyah Puspitasari
Journal of Science and Education Research Vol. 4 No. 1 (2025): Journal of Science and Education Research
Publisher : Yayasan Pendidikan Insan Mulia Utan Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62759/jser.v4i1.192

Abstract

Challenges in learning biology often arise, especially due to the complex nature of the material and requires a deep understanding of interrelated concepts. This causes many students to experience decreased motivation. The biology teacher of SMA NW Kopang keeps students motivated in learning. This study aims to understand the strategies applied by biology teachers at SMA NW Kopang in increasing students' learning motivation. This research uses a qualitative approach with the methods of observation, in-depth interviews, and document analysis. The research findings show that teachers use various strategies such as contextual learning, the use of educational technology, personalised approaches, as well as the insertion of ice breaking and rewards to create an interactive and relevant learning atmosphere. In addition, the integration of Islamic values in biology learning provides a moral dimension that strengthens the relationship between science and student character. Internal factors such as student interest and confidence, as well as external factors such as teacher support and learning environment, were found to have a significant influence on learning motivation. These strategies not only help students understand complex biological material, but also encourage their active involvement in learning. This research contributes to the development of learning methods that are innovative and relevant to students' needs, particularly in Islamic-based schools. Further research is recommended to explore the long-term impact of these strategies on students' learning outcomes across different educational contexts.
Diversity and Potential of Local Actinobacteria from Bedengan Forest for Sustainable Antibiotic Solutions Sakaroni, Rubiyatna; Azizah, Isnaniar Rahmatul
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 5 No 1 (2025): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v5.i1.pp255-264

Abstract

Natural antibiotics may offer a solution to the growing issue of pathogenic bacterial resistance caused by the widespread use of synthetic antibiotics. This study investigates the diversity and potential of Actinobacteria from the rhizosphere of Pinus in the Selorejo Bedengan Forest, Malang City, which has been exposed to synthetic agricultural chemicals for an extended period. Soil samples were collected and analyzed for various environmental factors, including pH, moisture, organic matter, and light intensity. A total of seven Actinobacteria isolates were identified, belonging to the genera Streptomyces and Nocardia. The diversity of these isolates was assessed using the Shannon-Wiener index, yielding a moderate diversity value (H' = 2.74). Antibacterial activity was tested against Escherichia coli and Shigella dysenteriae using the pitting method. The results indicated that isolates AC5 and AC7 exhibited inhibition zones of ≥11 mm and ≥12 mm, respectively, categorizing them as having strong antibacterial activity. These findings highlight the significant potential of local Actinobacteria as candidates for the production of natural antibacterial compounds to combat pathogenic bacteria. This research not only supports the development of more environmentally friendly antibiotic alternatives but also encourages the sustainable use of local microbial resources.
Pelatihan Tentang Model Akumulasi Logam Berat Cd dalam Tubuh Organisme pada Siswa MTs Yasim Nata Kabupaten Bima Khairuddin; Yamin, M.; Kusmiyati; Bahri, Syamsul; Sakaroni, Rubiyatna
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 2 (2025): April-Juni 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i2.11562

Abstract

Penumpukkan logam berat Kadmium (Cd) pada makhluk hidup merupakan pengetahuan yang penting untuk dipahami oleh siswa sejak dari pendidikan dasar sampai perguruan tinggi termasuk siswa Madrasah Tsanawiah Yasim Nata Kabupaten Bima. Penyelenggaraan kegiatan dalam pengabdian kepada masyarakat ini berkaitan dengan adanya masalah yang dirasakan oleh mitra yaitu tentang bagaimana upaya yang seharusnya dilakukan agar dapat meningkatkan konsep keilmuan tentang logam berat Kadmium (Cd), dan keterampilan yang bagaimanakah yang perlu dimiliki dari awal oleh siswa Madrasah Tsanawiah Yasim Nata Kabupaten Bima agar model akumulasi Cd dalam tubuh organisme dapat dimengerti dan dipahami dengan benar. Tujuan dari kegiatan pelatihan tentang model akumulasi logam berat Cd yaitu agar pemahaman dan pengetahuan siswa dapat meningkatkan. Tujuan lain yaitu untuk meningkatkan sikap terampil dan wawasan pengetahuan agar terhindar dari paparan langsung dengan Cd dalam rutinitas sehari-hari, sehingga siswa Mts Yasim Nata Kabupaten Bima tidak terkena dampak dari akumulasi loam berat Cd. Pelaksanaan pelatihan ini dapat bermanfaat terhadap meningkatnya pengetahuan, pemahaman dan ketrampilan siswa terhadap model akumulasi logam Cd. Pelatihan ini juga bermanfaat bagi siswa dalam menemukan supaya terhindar dari paparan langsung dengan logam Cd dalam rutinitas harian kehidupan. Kesimpulan dari pelatihan ini yaitu munculnya peningkatan pengertian, tambahan ilmu pengetahuan dan keterampilan khususnya yang berhubungan dengan akumulasi logan berat Cd dalam tubuh organisme di MTs Yasim Nata Kabpaten Bima yang pada akhirnya dapat mengerti dengan baik tentang model akumulasi logam berat Cd dan cara menghindari diri dari kontaminasi logam Kadmium pada tubuh organisme dalam kehidupan sehari-hari
Pelatihan dan Pendampingan Pemberian Nama Ilmiah Pohon di Area Sekolah Untuk Optimalisasi Lingkungan Sekolah Sebagai Sumber Belajar di SMPN 17 Mataram Kusuma, Anindita Suliya Hangesti Mandra; Sakaroni, Rubiyatna; Hidayanti, Ermia; Suryaningsih, Hartati; Shafa, Mezaluna M; Parhana, Anggun SN; Marchila, Aura PA; Islamiyah, Nur
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 2 (2025): April-Juni 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i2.11567

Abstract

Pendidikan lingkungan hidup memiliki peran penting dalam membentuk karakter siswa yang peduli terhadap alam. Artikel ini membahas implementasi kegiatan pendampingan pemberian nama ilmiah pada tumbuhan di lingkungan SMPN 17 Mataram sebagai media pembelajaran berbasis lingkungan. Kegiatan ini dilatarbelakangi oleh rendahnya pemanfaatan tumbuhan sekolah sebagai sumber belajar, khususnya dalam pembelajaran Ilmu Pengetahuan Alam (IPA). Metode yang digunakan meliputi analisis masalah, observasi, pendataan tanaman, identifikasi nama ilmiah melalui referensi ilmiah, pelabelan tanaman, serta refleksi siswa. Hasil menunjukkan bahwa lebih dari 20 jenis pohon berhasil diidentifikasi dan dilabeli, seperti Mangifera indica dan Swietenia macrophylla. Proses ini meningkatkan literasi sains, keterampilan berpikir kritis, dan kesadaran ekologis siswa. Pemasangan label tanaman tidak hanya memperkaya sumber belajar kontekstual, tetapi juga mendukung pendekatan pembelajaran berbasis proyek dalam Kurikulum Merdeka. Kegiatan ini dinilai efektif dan berkelanjutan dalam membentuk karakter siswa yang cinta lingkungan, serta berpotensi menjadi program unggulan sekolah berbasis konservasi dan pendidikan ekologis.
Sosialisasi Hasil Brand Audit Sampah Pendakian kepada Masyarakat di Jalur Pendakian Tetebatu, Taman Nasional Gunung Rinjani Santoso, Didik; Adawiyah, Marosa Robi’atul; Sakaroni, Rubiyatna; Setiawan, Heru
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 1 (2025): Januari-Maret 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i1.12097

Abstract

Permasalahan sampah di jalur pendakian Tetebatu, Taman Nasional Gunung Rinjani, menunjukkan perlunya keterlibatan masyarakat lokal dalam upaya pengurangan limbah konsumsi. Kegiatan sosialisasi ini dilaksanakan di Desa Tetebatu, Kecamatan Sikur, Kabupaten Lombok Timur, dan bertujuan untuk menyampaikan hasil analisis brand audit barang berpotensi sampah sebagai bentuk edukasi lingkungan kepada masyarakat. Sosialisasi dilakukan selama satu hari dan melibatkan warga sekitar jalur pendakian, seperti porter, pedagang, dan pelaku usaha lokal. Materi disampaikan melalui visualisasi data serta diskusi terbuka mengenai produk-produk yang paling banyak menyumbang sampah di jalur pendakian. Hasil kegiatan menunjukkan peningkatan pemahaman masyarakat terhadap dampak lingkungan dari produk sekali pakai, serta munculnya inisiatif penggunaan kemasan yang lebih ramah lingkungan. Kegiatan ini menunjukkan bahwa penyampaian hasil riset dalam format sederhana dan partisipatif dapat menjadi strategi efektif dalam mendorong pelestarian lingkungan berbasis komunitas.
Menakar Literasi Mikrobiologi Mahasiswa Pendidikan Biologi Universitas Mataram Sakaroni, Rubiyatna; Wirdullutfi, Wirdullutfi; Hijriani, Baiq Isti
Journal of Science and Education Research Vol. 4 No. 2 (2025): Journal of Science and Education Research
Publisher : Yayasan Pendidikan Insan Mulia Utan Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62759/jser.v4i2.316

Abstract

Microbiology literacy is an important skill for prospective biology teachers to be able to understand, teach, and apply microbiology concepts appropriately in life and learning. This study aims to describe the microbiology literacy profile of biology education students at Mataram University based on competency and attitude dimensions after completing microbiology courses. The study employs a quantitative descriptive approach using instruments developed based on the PISA 2015 science literacy framework. The research subjects were students who had taken microbiology courses. Data were analyzed descriptively to determine literacy score categories. The results showed that students' microbiology literacy was generally in the moderate to low category. In the competency dimension, the highest scores were in the ability to interpret data and scientific evidence, while the lowest scores were in the ability to evaluate and design scientific investigations. In the dimension of attitude, the majority of students were in the moderate category, with the highest scores in the assessment of scientific approaches and the lowest scores in interest in microbiology. These low scores indicate the need to strengthen higher-order thinking skills, hands-on experience in research design, and learning that links microbiology concepts to real-life contexts. This study recommends the use of project-based, problem-based, and socio-scientific issue-based learning strategies, as well as evaluations that require the application of concepts and analysis. This approach is expected to enhance students' conceptual understanding, analytical skills, and scientific attitudes in a balanced manner.
ANALYSIS OF ACTINOMYCETES ABUNDANCE IN THE RHIZOSPHERE OF MANGROVE ECOSYSTEMS IN SEKOTONG AS A POTENTIAL SOURCE OF BENEFICIAL MICROBES Sakaroni, Rubiyatna; Baiq Isti Hijriani; Wirdullutfi
Jurnal Biogenerasi Vol. 10 No. 3 (2025): Volume 10 no 3 tahun 2025 terbit september
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i3.6701

Abstract

The rhizosphere of mangroves is a microhabitat rich in root exudates and organic matter, making it an ideal environment for the growth of Actinomycetes. This study aims to analyze the abundance of Actinomycetes in the mangrove rhizosphere at three different locations in Sekotong District, West Lombok: Bagek Kembar, Tanjung Batu, and Buwun Mas. Sampling was conducted using purposive sampling in the dominant root zone at a depth of ±20 cm. Actinomycetes isolation was performed using the surface plate method on SCA medium supplemented with nystatin as an antifungal agent. The results showed that the highest abundance was found in the rhizosphere of Rhizophora mucronata at Bagek Kembar with 4.6 × 10⁵ CFU/g, while the lowest was in Avicennia marina at Tanjung Batu with 1 × 10⁵ CFU/g. In general, Rhizophora mucronata showed higher abundance than Avicennia marina in all locations. This difference was influenced by root exudate characteristics, root system structure, and soil physicochemical conditions such as aeration and organic matter content. This study concludes that the rhizosphere of Rhizophora mucronata in Bagek Kembar is a more supportive habitat for the growth of Actinomycetes. This finding serves as an important basis for further exploration of Actinomycetes isolates from mangroves as potential sources of biocontrol agents and antimicrobial compound producers.
Actinobacteria from Mangrove Rhizosphere as a Source of Biocontrol Agents to Support Sustainable Agriculture Sakaroni, Rubiyatna; Kusuma, Anindita Suliya Hangesti Mandra; Adawiyah, Marosa Robi'atul
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 5 No 2 (2025): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v5.i2.pp360-369

Abstract

Mangrove ecosystems harbor diverse microorganisms with important ecological and biotechnological roles. Actinobacteria, in particular, are well known as producers of bioactive compounds and potential biocontrol agents against plant pathogens, making their exploration relevant for sustainable agriculture. This study aimed to isolate and characterize Actinobacteria from the rhizosphere of mangroves in Bagek Kembar, West Lombok, and to evaluate their antagonistic potential against Ralstonia solanaciearum, plant disease agent. Soil samples were collected using a quadrant method, while environmental parameters such as pH, temperature, humidity, and salinity were measured to assess habitat suitability. Actinobacteria were isolated and identified through Gram staining and morphological observation, their abundance was calculated using the Total Plate Count method, and antagonistic activity was tested using the agar well diffusion assay. The results showed that soil pH was relatively neutral, with temperature and salinity suitable for Actinobacteria growth. Five isolates (ACT R1–R5) were obtained, all Gram-positive with filamentous hyphae-like morphology. Total Plate Count analysis indicated high abundance (3.2 × 105), although the diversity of isolates recovered was relatively low. Antagonistic assays revealed that ACT R2 and ACT R4 moderate inhibitory effect, and ACT R1, ACT R3, and ACT R5 weak. Overall, these findings demonstrate that mangrove rhizospheres in West Lombok are a promising source of Actinobacteria with significant potential as environmentally friendly biocontrol agents
Bibliometric Insights on Mangrove Actinobacteria's Secondary Metabolites as Antibiotics Using VOSviewer Sakaroni, Rubiyatna; Listantia, Nora; Ningthias, Dyah Puspitasari
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8147

Abstract

Secondary metabolites from actinobacteria have been known to have many potentials as antioxidants, antibiotics, anti-cancer, and others. Various studies have been conducted and published regarding the findings of the potential of actinobacteria. This study aims to determine the development of research related to the potential of actinobacteria originating from mangrove areas and their secondary metabolites as natural antibiotics with bibliometric methods assisted by the VOS Viewer application. The articles used in this study are articles published from 2014 to 2024 and taken from the PubMed database. A total of 78 articles analyzed with VOS Viewer showed that over the past decade, the development of research on this topic still revolves around actinobacteria, microbial sensitivity tests, anti-bacterial agents, and Streptomyces as indicated by the high occurrence of these words in the titles and abstracts of the articles analyzed. The potential for research development can be directed at antineoplastic agents, cell proliferation, and mass spectrometry because the use of these terms is still minimal. However, analysis using other databases is needed as a comparison to get clearer results related to the development of research on the potential of actinobacteria derived from mangroves and their secondary metabolites as antibitoics.
Co-Authors Adawiyah, Marosa Robi'atul Adawiyah, Marosa Robi’atul Aini, Jannatul Ayu Citra Rasmi, Dewa Azizah, Isnaniar Rahmatul Baiq Mulianah BaiqIsti Hijriani Cahyadi Wirajagat, Gde Didik Santoso Fadilla Yuliana, Baiq Heru Setiawan Hidayanti, Ermia Ilhamdi, Mohammad Liwa Imam Bachtiar ISLAMIYAH, NUR Kamarudin Zaelani, Kamarudin Karoluslina Khairuddin Kusmiyati Kusuma, Anindita SHM Kusuma, Anindita Suliya Hangesti Mandra LALU ZULKIFLI Lianto Listantia, Nora M. Yamin Marchila, Aura PA Muhlis Muslihatun, Fitri Ni Luh Putu Pebri Artayani . Ningthias, Dyah Puspitasari Nuraisyah Parhana, Anggun SN Rasmi, Dewa Ayu Citra Rispawati, Dewi Robi'atul Adawiyah, Marosa Sartika Hijriati Shafa, Mezaluna M Supriastuti, Eko Suryaningsih, Hartati Syamsul Bahri Wirajagat, Gde Cahyadi Wirdullutfi, Wirdullutfi