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All Journal Majalah Obat Tradisional Sinteza
Khairurrizki, Amanda
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Cytotoxic Potential of Combination of Hibiscus sabdariffa L. Extract and Cisplatin against HeLa Cervical Cancer Cell Line: A Study of Antiproliferative Activity and Apoptosis Induction Nurani, Laela Hayu; Azzahra, Fara; Utami, Dwi; Edityaningrum, Citra Ariani; Guntarti, Any; Irham, Lalu Muhammad; Daud, Intan; Khairurrizki, Amanda; Putra, Ichsan Luqmana Indra; Ismiyati, Nur; Rofida, Siti; Sugiyanto, Sugiyanto
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.92440

Abstract

Cervical cancer is one of the most prevalent cancer types, making the development of effective anticancer agents critical. Cisplatin (CIS) is a drug that has been used to treat cancer, but it also affects normal cells. Hibiscus sabdariffa L. extract (HSE), which has the potential as an anticancer agent, can be developed as a co-chemotherapy with CIS. This study aimed to determine the potential of HSE as a co-chemotherapy with CIS against HeLa cervical cancer cells and determine specific and non-specific parameters of the studied extract. Hibiscus sabdariffa L. (HS) simplicia powder was macerated with 96% ethanol. An in vitro evaluation was carried out on antiproliferative activity by calculating the doubling time. Additionally, a selectivity test was conducted to calculate combination index (CI) values based on the microtetrazolium (MTT) method. Apoptosis mechanisms were explored based on immunocytochemical methods using p53, caspase, and Bax antibodies, followed by an observation of apoptotic induction using a flow cytometer. The HSE and CIS selectivity index values obtained for Vero cells and HeLa cells were 209 and 278, respectively. The antiproliferation test results showed that the combination of HSE and CIS could better extend the doubling time of cells compared to the negative control. This combination also demonstrated a strong synergistic effect, with a CI value of 0.001. The extract as a co-chemotherapy with CIS was capable of increasing the expression of p53, caspase-3, and Bax. The flow cytometry analysis results indicated that HSE could induce apoptosis. Based on the results on the IC50 and CI value of HSE, as well as on the doubling time and apoptosis induction of HSE-influenced HeLa cells, it is concluded that HSE has the potential as a co-chemotherapy against cervical cancer.
Efek Sinergisme Ekstrak Daun Pegagan (Centella asiatica L.) dengan Obat Antidiabetik Oral terhadap Penurunan Kadar Glukosa Darah Puasa pada Mencit Khairurrizki, Amanda; Rafsanjani, Arief; Hariadi, Puspawan
Sinteza Vol. 2 No. 1 (2022): February
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.586 KB) | DOI: 10.29408/sinteza.v2i1.4385

Abstract

Tanaman pegagan (Centella asiatica L.) mengandung senyawa triterpenoid dan flavonoid yang memiliki mekanisme penurunan kadar glukosa dalam darah dan aktivitas antioksidan. Penelitian ini bertujuan untuk mengetahui efek sinergisme antara ekstrak daun pegagan dengan obat antidiabetik oral dalam menurunan kadar glukosa darah puasa pada mencit. Desain penelitian ini menggunakan pre dan post test control group design. Induksi hiperglikemia pada mencit menggunakan aloksan, pengujian pada mencit terbagi dalam 12 kelompok, diantaranya kontrol negatif, kontrol positif (metformin dan glibenklamid), dan kelompok perlakuan pertama (I,II,IIII), kedua (IV, V, VI), dan ketiga (VII, VIII, IX) yang diberikan ekstrak daun pegagan dengan 3 dosis yang berbeda, yaitu 56,7 mg/kgBB; 113,4 mg/kgBB; dan 170,1mg/kgBB. Pada kelompok perlakuan kedua, ekstrak daun pegagan dikombinasikan dengan metformin 1,75 mg/kgBB dan glibenklamid 0,0175 mg/kgBB pada kelompok perlakuan ketiga.  Data dianalisis menggunakan Oneway Anova dan uji lanjut LSD. Hasil analisis menunjukkan terdapat efek sinergisme pada kelompok perlakuan V (Ekstrak Daun Pegagan 113,4 mg/kgBB dengan metformin 1,75 mg/kgBB), dimana terdapat penurunan kadar glukosa darah puasa yang signifikan dibandingkan penurunan kadar ekstrak tunggal, yaitu 93 mg/dL dan 77,66 mg/dL. Kesimpulan dari penelitian ini adalah kombinasi ekstrak etanol daun pegagan pada dosis 113,4 mg/kgBB dengan 1,75 mg/kgBB metformin merupakan dosis yang paling efektif dalam menurunkan kadar glukosa darah puasa.
Co-Authors Any Guntarti Arief Rafsanjani Azzahra, Fara Citra Ariani Edityaningrum, Citra Daud, Intan Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Laela Hayu Nurani Lalu Muhammad Irham Muhammad Yusron Maulana El-Yunusi Nur Ismiyati Puspawan Hariadi Siti Rofida Sugiyanto -