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All Journal Caraka Tani: Journal of Sustainable Agriculture Jurnal Biologi Tropis
Tsaniyah, Barokati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Water Content, Physical Quality of Seeds and Post-Harvest Fungal Attacks on Peanuts Miladiarsi, Miladiarsi; Tsaniyah, Barokati; Putri, Riska Awalia; Saputra, Hiksa Maulana; Sianturi, Yosua Pangihutan Pardamean Alextio
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9754

Abstract

The decline in peanut seed quality can result from improper post-harvest handling, including drying, pod peeling, and storage, which can damage the seeds and reduce their quality. Damaged seeds and high water content facilitate fungal growth on peanuts. Maintaining and ensuring peanut quality during market storage is very important. This study aims to examine the relationship between air content, the percentage of damaged seeds, and the percentage of seeds infected by post-harvest fungal species in peanut seeds. The experimental method involved measuring air content with a DELMHORST Model G-7 Moisture Meter. The physical quality of seeds was assessed based on the percentages of intact, wrinkled, and damaged seeds. To determine the percentage of seeds infected with fungi, the direct planting method was used, employing Dichloran 18% Glycerol Agar (DG18+) media. The results indicated that each sample’s air content was within the normal range of 8-9%, but the physical quality of the seeds was 59.7%, associated with fungal attacks by species including Aspergillus flavus, A. niger, A. tamari, Fusarium semitechtum, Mucor sp., Eurotium repens, and E. chevalieri. These findings suggest that air content and seed damage influence fungal diversity, providing valuable insights for maintaining peanut seed quality during the post-harvest process until they reach the market.
Identification of Pathogens Causing Important Diseases in Leatherleaf Fern (Rumohra adiantiformis) and In Vitro Inhibition using Bacillus velezensis B-27 Tsaniyah, Barokati; Joko, Tri; Widiastuti, Ani
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.83675

Abstract

Leatherleaf fern (Rumohra adiantiformis) is a famous ornamental-leaf plant that has been used by florist entrepreneurs around the world. It is one of the leading export commodities in Indonesia, however, currently, there are some diseases of this leaf reported in the field causing yield loss and reducing the economic value. This study aimed to identify the pathogens causing the recent 3 significant diseases of leatherleaf fern, including leaf blight, leaf tip rot, and post-harvest leaf rot, and in vitro analysis of beneficial bacteria, Bacillus velezensis B-27, against the pathogens. The methods used in this study were isolation, pathogenicity test, morphological observation, molecular identification of pathogens, and poisoned food technique of B. velezensis against those pathogens compared to fungicides and bactericides. The results of molecular identification showed that Neopestalotiopsis sp. and Pantoea ananatis caused leaf blight, Fusarium oxysporum f. sp. sesami triggered leaf tip rot, while Calonectria sp. and P. ananatis contributed to post-harvest leaf rot. Based on in vitro analysis, B. velezensis B-27 reduced the growth of the Neopestalotiopsis sp. DM C with the highest inhibition of 95.6%, Neopestalotiopsis sp. DM B with 84.3%, F. oxysporum f. sp. sesami with 61.9%, Calonectria sp. with 93.4%, and inhibited the growth of P. ananatis by producing a clear zone. This research concludes that B. velezensis B-27 has the potential as a biocontrol against pathogens causing significant diseases in leatherleaf ferns due to its ability to inhibit pathogens and its advantage as a beneficial microbe that is environmentally friendly to support sustainable agriculture.
Co-Authors Ani Widiastuti, Ani Hiksa Maulana Saputra Miladiarsi Putri, Riska Awalia Sianturi, Yosua Pangihutan Pardamean Alextio Tri Joko