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Tem Gene Detection as ESBL Marker in Clinical Isolates Klebsiella pneumoniae : A Case Study Arafah Nurfadillah; Wahdaniar; Miladiarsi; Ade Irma; Tenri Ayu Adri
Journal of Health Sciences and Medical Development Vol. 1 No. 02 (2022): Journal of Health Sciences and Medical Development
Publisher : The Indonesian Institute of Science and Technology Research

Klebsiella pneumoniae is one of the most common ESBL-producing microorganisms. The research aims to detect the Tem gene in clinical isolates of K. pneumonaie from the Laboratory of clinical pathology of Wahidin Sudirohusodo General Hospital. Methods: This research is a descriptive cross-sectional design. Fifty samples of K. pneumonieae were obtained from the Laboratory of clinical pathology from March to May 2018. Tem gene was detected using primary Tem 164.SE and Tem 165.AS (445 bp) of Polymerase Chain Reaction (PCR). The research results indicate that 48 isolates are positive, containing the Tem gene with the highest number found in the sputum specimen. Genotype detection of bacteria-producing ESBL is essential so that antibiotic therapy given to the patients is more effective and efficient.

Pelatihan Pembuatan Minuman Kombucha Sebagai Upaya Peningkatan Fungsi Fisiologis Tubuh di Kantor Desa Moncongloe Bulu’ Kecamatan Mongcongloe Kab Maros Wahdaniar; Ade Irma; Miladiarsi
Jurnal Nusantara Berbakti Vol. 1 No. 1 (2023): Januari : Jurnal Nusantara Berbakti
Publisher : Universitas Kristen Indonesia Toraja

Pada tanggal 7 Desember 2022 telah dilakukan kegiatan pengabdian kepada masyarakat dalam bentuk penyuluhan dan cara pembuatan Kombucha. Pengabdian ini bertujuan untuk memberikan edukasi kepada mayarakat Desa Moncongloe Bulu’ kecamatan Moncongloe Kabupaten Maros tentang manafaat Kombucha dalam peningkatan fungsi fisiologi tubuh.Metode yang dilakukan dalam pengabdian ini yaitu memberikan solusi dan pengetahuan khususnya di bidang kesehatan mengenai manfaat Kombucha. Peserta dalam program pengabdian ini sangat antusias dalam menerima dan melaksanakan tahapan yang dipaparkan. Peserta pada pengabdian ini adalah pegawai kantor desa, ibu PKK, KadeR Desa dan Mahasiswa KKN. Tahapan-tahapan yang dilaksanakan pada program pengabdian ini telah membantu meningkatkan pengetahuan masyarakat Desa Moncongloe Bulu’ khususnya di Kacematan Moncongloe mengenai proses pembuatan Kombucha dan mengetahui manfaat kesehatan yang di hasilkan dalam mengkomsumi dan menggunakan Kombucha.

Potential of Harum Manis Mango (Mangifera Indica L.) Seed Extract for Nosocomial Infections Wahdaniar; Imran Pashar; Miladiarsi; Ade Irma
Journal of Public Health Sciences Vol. 2 No. 02 (2023): Journal of Public Health Sciences
Publisher : The Indonesian Institute of Science and Technology Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56741/jphs.v2i02.213

Nosocomial infections occur in hospitals and attack patients who are in the recovery process and have low immunity. The emergence of nosocomial conditions in the hospital environment is caused by bacteria and moist objects or materials, especially in the operating room and even the treatment room for children and babies. One type of bacteria that causes the infection is Pseudomonas aeruginosa. P. aeruginosa has been recognized as an irreversible problem in hospitals due to resistance to 3 antibiotic classes. The content of sweet mango seeds has a high phytochemical content in the form of steroid compounds, terpenoids, flavonoids, alkaloids, phenolics, tannins and saponins. This study aimed to determine the potential of sweet mango seed extract against nosocomial infections. The research method is quantitative experimental, using ethanol extract of sweet fragrant mango seeds with well diffusion and dilution tests (MIC and MBC). The results of the antibacterial activity test measured the inhibition zone formed and tested MIC and MBC. MIC value of sweet fragrant mango seed extract showed at 1.56 mg/mL and 0.78, and at MBC value of 25 mg/mL and 50 mg/mL. The results of sweet fragrant mango seed extract have the potential to be developed as an antibacterial agent, especially against nosocomial infections.

Efektivitas Antimikroba Bakteri Probiotik Dari Usus Itik Pedaging Anas Domesticus terhadap Pertumbuhan Vibrio Spp Ade Irma; Wahdaniar Wahdaniar; Miladiarsi Miladiarsi
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Telah dilakukan penelitian â€œEfektivitas Antimikroba Bakteri Probiotik dari Usus Itik Pedaging Anas domesticus terhadap Pertumbuhan Vibrio spp.â€. Penelitian ini bertujuan untuk mengetahui kemampuan bakteri probiotik dari usus itik pedaging Anas domesticus berdasarkan perbedaan lama inkubasi kultur probiotik terhadap pertumbuhan Vibrio spp. Kemampuan dari isolat H terhadap pertumbuhan Vibrio spp. dapat diketahui dengan melakukan uji daya hambat. Berdasarkan uji daya hambat yang telah dilakukan menunjukkan bahwa isolat H memiliki kemampuan dalam membunuh atau bersifat bakteriosidal terhadap pertumbuhan Vibrio spp dengan membentuk zona bening disekitar blank disk. Indikasi ini membuktikan bahwa isolat H mampu menghasilkan senyawa metabolit selama pertumbuhannya. Lama inkubasi kultur isolat H berpengaruh terhadap senyawa metabolit yang dihasilkan. Produksi optimum senyawa metabolit berada pada kondisi kultur 48 jam yang ditunjukkan dengan adanya penambahan zona bening disekitar blank disk. Isolat H juga memiliki kemampuan yang tinggi terhadap pertumbuhan Vibrio harveyi dibandingkan dengan V. parahaemolyticus dan V. cholera.

Uji Diagnostik Jamur Dermatofita Pada Luka Kaki Penderita Diabetes Melitus dengan Metode PCR (Polymerase Chain Reaction) Miladiarsi Miladiarsi; Indas Wari Rahman; Santi Santi; Nurfardila Nurfardila
Jurnal Biotek Vol 11 No 1 (2023): JUNI
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v11i1.31707

Copyright©2023 Diabetic wounds often occur in people with diabetes mellitus as a result of impaired tissue perfusion and fungal infections, one of which is excessive dermatophytes that cause tissue death. This study aims to detect dermatophyte fungi in foot wounds of diabetics with diabetes mellitus by the Polymerase Chain Reaction (PCR) method. The type of laboratory observational study with a cross sectional study design, and the sampling technique used is total sampling by swab foot wounds in diabetes mellitus patients. The research method uses the PCR method to detect the presence or absence of dermatophyte fungi characterized by the formation of DNA fragments on electrophoresis visualization. The results showed that from 8 samples detected, 5 samples or 62.5% were positive for dermatophyte fungal infection based on amplification results marked by the formation of an amplicon DNA target band measuring around 700-1000 bp using ITS1 primers (forward: 5' GGT TGG TTT CTT TTC CT 3') and ITS2 (reverse: 5' AAG TAA AAG TCG TAA CAA GG 3'), so it can be concluded that in identifying samples using the PCR (Polymerase Chain Reaction) method has a high sensitivity in detecting fungi so as to allow more specific and more accurate results.

PMP Pencegahan Stunting Melalui Edukasi dan Skrining Kecacingan pada Ibu Hamil di Desa Biring Je’ne Moncongloe Hasriani Saleng; Miladiarsi; Ayu Lestari
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 4 No. 3 (2023): September
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) AMIK Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35870/jpni.v4i3.473

Stunting is a failure in children's growth and development experienced by children under the age of five due to a lack of nutritional intake when the fetus is in the womb until the beginning of the baby's birth and begins to appear until the baby is two years old. Mothers' lack of knowledge is a risk factor for stunting and providing education is one way to overcome stunting. The aim of this service is to increase public knowledge and awareness, especially for pregnant women, to find out about efforts to prevent stunting. The service method is carried out by providing education through counseling. The participants who attended this service were pregnant women, 40 participants took part in the service. Univariate data analysis using a descriptive approach and bivariate testing using the Paired T test. The results of service are increased knowledge after providing education. The test results obtained a P Value of 0.000 or there was an influence of education on participants' knowledge.

PMP Pencegahan Stunting Melalui Edukasi dan Skrining Kecacingan pada Ibu Hamil di Desa Biring Je’ne Moncongloe Hasriani Saleng; Miladiarsi; Ayu Lestari
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 4 No. 3 (2023): September
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35870/jpni.v4i3.473

Stunting is a failure in children's growth and development experienced by children under the age of five due to a lack of nutritional intake when the fetus is in the womb until the beginning of the baby's birth and begins to appear until the baby is two years old. Mothers' lack of knowledge is a risk factor for stunting and providing education is one way to overcome stunting. The aim of this service is to increase public knowledge and awareness, especially for pregnant women, to find out about efforts to prevent stunting. The service method is carried out by providing education through counseling. The participants who attended this service were pregnant women, 40 participants took part in the service. Univariate data analysis using a descriptive approach and bivariate testing using the Paired T test. The results of service are increased knowledge after providing education. The test results obtained a P Value of 0.000 or there was an influence of education on participants' knowledge.

Isolation and Identification of Lactic Acid Bacteria in Sinjai Typical Drinks (MINAS) Miladiarsi, Miladiarsi Mila; Wahdaniar, Wahdaniar
Biomedika Vol 17 No 1 (2024): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v17i1.1867

A fermented drink produced locally in the Sinjai area that is well known in the local community as well as widely is Minas. Minas, which means a typical Sinjai drink, used to be known as Irex. However, over time this drink was replaced with the name Minas because it was only produced in Sinjai City and used as a typical Sinjai drink has properties and benefits to increase body stamina due to fatigue after doing daily activities or work. Minas are made from cassava tapai, young coconut, eggs, coconut water, sugar water, honey, and milk and can be added with other fruits such as durian. Curved tape is a type of food produced from the fermentation process of substrates or carbohydrate foodstuffs using yeast yeast which belongs to the group of lactic acid bacteria. To determine the type of lactic acid bacteria that play a role in the fermentation process of minas drinks by isolating and identifying morphology using the gram staining method, biochemical tests using catalase tests, gas production and carbohydrate fermentation tests. Furthermore, molecular identification uses 16S RNA. The DNA sequencing results were analyzed with MEGA 6 software with the BLASTn algorithm at NCBI. Based on the results of the identification of lactic acid bacteria, homologous nucleotides were detected with the bacteria Bacillus subtilis, Bacillus and Streptococcus sp which are a group of lactic acid bacteria that are able to ferment carbohydrates to produce lactic acid.

Pengaruh Pencucian Luka menggunakan Infus Daun Sirih Merah (Piper Crocatum) 40% terhadap Proses Penyembuhan Ulkus Diabetik Wahdaniar Wahdaniar; Imran Pashar; Miladiarsi Miladiarsi
Jurnal Biomedika dan Kesehatan Vol 5 No 3 (2022)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2022.v5.153-160

BackgroundDiabetes mellitus (DM) is a chronic metabolic disease that can cause various complications. World Health Organization data for 2019, DM is the direct cause of 1.5 million deaths, and 48% of all deaths due to diabetes occur at the age of 70 years. Data from the International Diabetes Federation (IDF), in 2021, Indonesia will be the fifth largest country with DM sufferers, with a total of around 19.64 million and an increase of about 81.8% compared to 2019. One of the complications that can be caused by diabetes mellitus is diabetic ulcers. Diabetic ulcer treatment requires a long time. Proper wound washing is an important factor in wound healing. Red betel leaf infusion can be used as a liquid to wash wounds. This study aims to analyze the effect of wound washing using 40% red betel leaf infusion on the healing process of diabetic ulcers. MethodsThe type of research used is quantitative research using the quasi method - experiment one group pre-post test. The research design is purposive sampling. This research was conducted SeptemberNovember 2017 at Semarang Independent Practice Nurses (PPM) consisting of 10 respondents. ResultsThe test results obtained a p-value of 0.024 (<0.05), meaning that there is an effect of giving red betel leaf infusion more effectively in wound healing. Therefore, this study recommends that nurses washing diabetic ulcer wounds can use 40% red betel leaf infusion. ConclusionThere is an effect of washing wounds using 40% red betel leaf infusion on the healing process of diabetic foot ulcers with a p-value of 0.024 (<0.05).

MOLECULAR DOCKING AND ADME STUDIES OF STIGMASTEROL COMPOUNDS AS ANTI-BREAST CANCER Amir, Nur Insani; Nurfadillah, Arafah; Miladiarsi, Miladiarsi; Irma, Ade; Wahdaniar, Wahdaniar
Indonesian Journal of Pure and Applied Chemistry Vol 8, No 1 (2025)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v8i1.83224

Molecular docking studies of stigmasterol (test ligand) and estradiol (control ligand) against the ER-Î± receptor were performed using PyRx software. This study aims to determine the interaction between stigmasterol ligands and ER-Î± receptors by the molecular docking method using PyRx. The study results show that stigmasterol and estradiol have the same binding energy value of -7.3 kcal/mol. The stigmasterol compound could therefore be used as a candidate for breast cancer drugs.

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