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Phytochemical Screening and Thin Layer Chromatography Analysis of Ethanol Extract of Bangle Rhizome (Zingiberis cassumunar Roxb.) Ananda Choirinisa Eka Putri; Perdana Priya Haresmita; Indra Yudhawan; Diyah Tri Utami; Alfian Syarifuddin
Jurnal Ilmu Kesehatan Vol 13 No 1 (2025): Jurnal Ilmu Kesehatan
Publisher : Universitas Muhammadiyah Kalimantan Timur

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Abstract

Bangle rhizome (Zingiber cassumunar Roxb.) is a part of the plant that is widely used in traditional medicine because its diverse secondary metabolites have great potential in treating various diseases. The aim of this research is to identify the content of curcumin and active compounds found in bangle rhizome (Zingiber cassumunar Roxb.) qualitatively and quantitatively using Thin Layer Chromatography and UV-Vis Spectrophotometry methods. The maceration method was used to extract the bangle rhizome with 70% ethanol solvent, resulting in an extract yield of 7.92%. The results of the study from qualitative analysis using Thin Layer Chromatography (TLC) with 3 different mobile phases, namely chloroform: ethanol (95:5), chloroform: methanol: glacial acetic acid (94:5:1), and chloroform: n-hexane: methanol (1:1:0.1), indicate that the ethanol extract from the bangle rhizome positively contains polyphenolic compounds, terpenoids, and glycosides. Quantitative analysis of curcumin using UV-Vis Spectrophotometry at a wavelength of 437 nm resulted in a linear equation y = 0.026x + 0.1026 with an R² value of 0.9958 and r = 0.997898. The absorbance values from the 3 replicates of the bangle rhizome ethanol extract sample solution obtained in this study were 0.35 nm; 0.38 nm; 0.392 nm, indicating the presence of curcumin in all three replicates of the bangle rhizome ethanol extract solution. Based on the research results, it can be concluded that the ethanol extract of bangle rhizome (Zingiber cassumunar Roxb.) positively contains polyphenolic compounds, terpenoids, glycosides, and curcumin.
Karakterisasi Simplisia, Ekstrak dan Spektra Infra Merah Senyawa Analgesik Daun Dadap Serep (Erythrina Subumbrans) Dari Kabupaten Magelang Arief Kusuma Wardani; Perdana Priya Haresmita; Tesia Aisyah Rahmania; Missya Putri Kurnia Pradani
Jurnal Farmasi Indonesia Vol 22 No 1 (2025): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/jfi.v22i1.2702

Abstract

Medicinal raw materials from agricultural products or groups of wild plants cannot be guaranteed; their chemical content is always constant due to variations in seedlings, body location, climate, or raw material conditions (age and harvest method). The content of the chemical compounds responsible for the biological response must have a chemical specification, i.e., information about the composition of both type and content. Standardization was carried out on specific parameters, including organoleptics, yield, the content of drying losses, water-soluble extract content, ethanol-soluble extract content, and chromatogram patterns. Non-specific parameters of the extract included water content, total ash content of powder and extract. The method of determination is carried out in accordance with the procedures established by Farmakope Herbal Indonesia (FHI 2017). Profiling has been done using infrared spectroscopy. Specific parameters showed that the organoleptic was a semisolid form with a dark green color, a distintive smell of dadap serep leaf extract, bitter sweetness, a yield of 7,63%, a drying loss of 7,51%, a water-soluble extract content of 4,86%, a level of ethanol-solubility extract content of 33,47%, and chromatogram patterns that were typical. Non-specific parameters indicate that the water content is 1,11%, acid-insoluble ash powder content 5,050 ± 0,036%; extract 0,986% and total ash powder content is 8,671 ± 0,303%; extract 4,123%. The results of parameter simplicia as well as ethanol extracts of leaves of Dadap Serep obtainable from the Magelang in accordance with the specifications in FHI 2017 and the spectroscopic photometer profile showing the typical pattern of extract and simplisia leaves.