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All Journal Jurnal Medika Veterinaria
Amukti, Pandu Tokoh
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Molecular Detection of Second Internal Transcribed Spacer (ITS-2) Encoding Gene Sarcoptes scabiei of Stray Cat from Several Market in Bondowoso Rusdiana, Nur; Wibawati, Prima Ayu; Retno Lastuti, Nunuk Dyah; Amukti, Pandu Tokoh
Jurnal Medika Veterinaria Vol 19, No 2 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i2.47035

Abstract

Scabies is an infectious disease which is caused by Sarcoptes scabiei mites and considered as an emerging/re-emerging parasitic disease. Recent study using the Polymerase Chain Reaction (PCR) method has a higher sensitivity for detecting mites than using the skin scraping method. This study aims to detect the Second Internal Transcribed Spacer (ITS-2) encoding gene of S. scabiei. ITS-2 is a highly conserved area and always used to identify and differentiate host species infected by S. scabiei. S. scabiei isolates were obtained from skin scrapings from stray cat suspected of being infected with Scabies from Pasar Induk Bondowoso, Pasar Tamanan and Pasar Pujer. Detection on the presence of S. scabiei was carried out microscopically and then confirmed by molecular examination using the PCR method and visualized by electrophoresis on 2% agarose gel. The Polymerase Chain Reaction (PCR) results showed DNA bands at 304 bp Based on the result it can be concluded that samples are positively infected by Scabies can be detected by the ITS-2 encoding gene of Sarcoptes scabiei.
Co-Authors Nur Rusdiana Retno Lastuti, Nunuk Dyah Wibawati, Prima Ayu