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All Journal Veterinary Biomedical and Clinical Journal Jurnal Medika Veterinaria
Retno Lastuti, Nunuk Dyah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Detection of Ectoparasites in Farmed Striped Catfish (Pangasius hypophthalmus) in Karangpilang, Surabaya, Indonesia Nadilla Chrisantya Yusuf Putri; Tri Suwanti, Lucia; Hadi Warsito, Sunaryo; Retno Lastuti, Nunuk Dyah; Rahardjo, Dadik; Aryaloka, Suhita
Veterinary Biomedical and Clinical Journal Vol. 5 No. 2 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.02.4

Abstract

This research aimed to identify ectoparasites infecting farmed striped catfish in Karangpilang Surabaya, Indonesia. Individual catfishes with visible lesions on the body surface and abnormal behaviors were included in the study. Mucilage samples of 90 fishes were examined under a microscope for the presence of ectoparasite on the body surface of the fish, in the area of gills, skins, fins, and tails. Gills were cut with scissors and combed with a tweezer. Skin and fin were also scrapped with a sterile scalpel. The mucus obtained was put on an object glass and was observed under the microscope. The fish pond water was analysed for Oxygen Demand (DO), pH and temperature three times two weeks apart, starting from the day of fish sampling. Results showed that 42.2% (n=90) of samples were infected by ectoparasites. The ectoparasites identified were Gyrodactylus sp., Dactylogyrus sp., Trichodina sp. and Chilodonella sp. Fish pond water in the study site was of suitable quality for the growth of catfish. This study suggested that pathogens other than ectoparasites play roles in the development of pathologic conditions of stripped catfishes in the study area.
Molecular Detection of Second Internal Transcribed Spacer (ITS-2) Encoding Gene Sarcoptes scabiei of Stray Cat from Several Market in Bondowoso Rusdiana, Nur; Wibawati, Prima Ayu; Retno Lastuti, Nunuk Dyah; Amukti, Pandu Tokoh
Jurnal Medika Veterinaria Vol 19, No 2 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i2.47035

Abstract

Scabies is an infectious disease which is caused by Sarcoptes scabiei mites and considered as an emerging/re-emerging parasitic disease. Recent study using the Polymerase Chain Reaction (PCR) method has a higher sensitivity for detecting mites than using the skin scraping method. This study aims to detect the Second Internal Transcribed Spacer (ITS-2) encoding gene of S. scabiei. ITS-2 is a highly conserved area and always used to identify and differentiate host species infected by S. scabiei. S. scabiei isolates were obtained from skin scrapings from stray cat suspected of being infected with Scabies from Pasar Induk Bondowoso, Pasar Tamanan and Pasar Pujer. Detection on the presence of S. scabiei was carried out microscopically and then confirmed by molecular examination using the PCR method and visualized by electrophoresis on 2% agarose gel. The Polymerase Chain Reaction (PCR) results showed DNA bands at 304 bp Based on the result it can be concluded that samples are positively infected by Scabies can be detected by the ITS-2 encoding gene of Sarcoptes scabiei.
Co-Authors Amukti, Pandu Tokoh Aryaloka, Suhita Dadik Rahardjo, Dadik Hadi Warsito, Sunaryo Nadilla Chrisantya Yusuf Putri Nur Rusdiana Tri Suwanti, Lucia Wibawati, Prima Ayu