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All Journal AGRIVITA, Journal of Agricultural Science
Yusuf, Hadi Mokhamad
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Agriculture, Biological Sciences & Forestry

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Rapid Micropropagation of Alocasia azlanii using Microshoot Tips as Explants Source Siregar, Ahmad Syahrian; Mas’udah, Shofiyatul; Firdiana, Elok Rifqi; Widiarsih, Sasanti; Budiarto, Kurniawan; Yusuf, Hadi Mokhamad; Yulianah, Izmi; Masnar, Adley L.
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.4838

Abstract

Alocasia azlanii is a unique species that has an ornamental value, native to Southeast Asia. The present investigation aimed to develop reliable in vitro propagation techniques for A. azlanii, suitable for potential mutation and polyploidization programs. The in vitro propagation protocol for these species includes sterilization, shoot proliferation, in vitro rooting induction, and acclimation stages. The results showed that the effective protocol for freeing the explants from contamination was achieved through additional pre-treatment with bactericide and fungicide, which produced the best percentage of clean shoot-tip explants (87%). At the shoot multiplication stage, the shoot-tip explant cultivated in MS medium supplemented with 0.05 mg/l TDZ and 1 mg/l BAP showed the highest number of shoots (31.8) in the second subculture cycle. In rooting stages, single shoots that were planted in MS medium supplemented with 0.5 mg/l activated charcoal and 0.5 mg/l NAA resulted in the best plantlet performances in terms of stem diameter, number of roots, number of leaves, and plantlet height. In the acclimatization stage, planting media composed of husk charcoal, soil, manure, and volcanic fine sand mixture (2:1:1:1 v/v) preserved vigorous plantlet development, with 93% survival.
Co-Authors Ahmad Syahrian Siregar, Ahmad Syahrian Elok Rifqi Firdiana, Elok Rifqi Izmi Yulianah Kurniawan Budiarto Masnar, Adley L. Mas’udah, Shofiyatul Sasanti Widiarsih