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All Journal AGRIVITA, Journal of Agricultural Science Bioeksperimen: Jurnal Penelitian Biologi Biosaintifika: Journal of Biology & Biology Education
Elok Rifqi Firdiana, Elok Rifqi
Master Program of Biology, University of Brawijaya
Agriculture, Biological Sciences & Forestry Education

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INVENTARISASI DAN STRATEGI PENATAAN KOLEKSI PTERIDOPHYTA DI RUMAH KACA KEBUN RAYA PURWODADI Renjana, Elga; Firdiana, Elok Rifqi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8580

Abstract

Pteridophyta merupakan salah satu koleksi tumbuhan yang dimiliki oleh Kebun Raya Purwodadi. Pemeliharaan koleksi Pteridophyta dilakukan di dalam rumah kaca karena iklim di Kebun Raya Purwodadi panas dan kering. Hasil inventarisasi menunjukkan bahwa Kebun Raya Purwodadi memiliki 36 jenis koleksi Pteridophyta yang terdiri atas 20 suku dan 38 marga. Suku yang paling banyak ditemukan adalah Nephrolepidaceae dan terdiri atas Nephrolepis bisserata Sw. Schott, N. cordifolia (L.) C. Presl., N. exaltata (L.) Schott, N. falcata (Cav.) C. Chr., N. hirsutula (G. Forst.) C. Presl., dan N. radicans (Burm.) Kuhn. Selain itu, Psilotum nudum (L.) P. Beauv. merupakan jenis koleksi Pteridophyta yang masuk pada IUCN Red List dengan kategori terancam punah. Untuk mempermudah proses pemeliharaan dan monitoring, dilakukan penataan koleksi Pteridophyta dengan pengelompokan berdasarkan marga dan diurutkan mulai kelompok paling primitif hingga modern. Hasil penataan ini dapat memberikan nilai edukasi kepada pengunjung Kebun Raya Purwodadi, khususnya para pelajar.
Morphological Characterization and Physical Dormancy of Bauhinia winitii Seed: Living Collection of Purwodadi Botanic Garden Lestari, Dewi Ayu; Firdiana, Elok Rifqi
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30501

Abstract

Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently included in endangered species list. B. winitii seeds are orthodox seeds in which it has hard coat morphologically. Therefore, B. winitii seeds often undergo physical dormancy which can be broken through immersion in certain liquid media. This study aimed to characterize the morphology of B. winitii seeds and determine the pre-sowing treatment method to accelerate the seeds germination of B. winitii. External morphological characterization was carried out by observing the seeds quantitative and qualitative parameters. Characterization of seeds internal morphology was conducted using a digital microscope. Physical dormancy breaking was carried out by immersion in warm and cold water for 24 hours. Morphological characterization data were analyzed descriptively, while seed germination data were analyzed quantitatively by using one-way ANOVA followed by LSD test (with  confidence level of 95%). B. winitii has pod-shaped fruits with 2-6 seeds per pod, the seeds are 1.36 cm x 1 cm in size, 0.47 cm thick and weighed 0.5 g, oblong to conical in shape, smooth and shine surface, with light to dark brown in color. Seeds immersion treatment in warm water could break the physical dormancy of B. winitii seeds thus 63% of the seeds were able to germinate and it was significantly different compared to control and cold water immersion treatment. Technical to germinate B. winitii seed can be known from pre-sowing treatment. This study can be used as a reference for seed identification and germination technical of B. winitii seed.
Rapid Micropropagation of Alocasia azlanii using Microshoot Tips as Explants Source Siregar, Ahmad Syahrian; Mas’udah, Shofiyatul; Firdiana, Elok Rifqi; Widiarsih, Sasanti; Budiarto, Kurniawan; Yusuf, Hadi Mokhamad; Yulianah, Izmi; Masnar, Adley L.
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.4838

Abstract

Alocasia azlanii is a unique species that has an ornamental value, native to Southeast Asia. The present investigation aimed to develop reliable in vitro propagation techniques for A. azlanii, suitable for potential mutation and polyploidization programs. The in vitro propagation protocol for these species includes sterilization, shoot proliferation, in vitro rooting induction, and acclimation stages. The results showed that the effective protocol for freeing the explants from contamination was achieved through additional pre-treatment with bactericide and fungicide, which produced the best percentage of clean shoot-tip explants (87%). At the shoot multiplication stage, the shoot-tip explant cultivated in MS medium supplemented with 0.05 mg/l TDZ and 1 mg/l BAP showed the highest number of shoots (31.8) in the second subculture cycle. In rooting stages, single shoots that were planted in MS medium supplemented with 0.5 mg/l activated charcoal and 0.5 mg/l NAA resulted in the best plantlet performances in terms of stem diameter, number of roots, number of leaves, and plantlet height. In the acclimatization stage, planting media composed of husk charcoal, soil, manure, and volcanic fine sand mixture (2:1:1:1 v/v) preserved vigorous plantlet development, with 93% survival.
INVENTARISASI DAN STRATEGI PENATAAN KOLEKSI PTERIDOPHYTA DI RUMAH KACA KEBUN RAYA PURWODADI Renjana, Elga; Firdiana, Elok Rifqi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8580

Abstract

Pteridophyta merupakan salah satu koleksi tumbuhan yang dimiliki oleh Kebun Raya Purwodadi. Pemeliharaan koleksi Pteridophyta dilakukan di dalam rumah kaca karena iklim di Kebun Raya Purwodadi panas dan kering. Hasil inventarisasi menunjukkan bahwa Kebun Raya Purwodadi memiliki 36 jenis koleksi Pteridophyta yang terdiri atas 20 suku dan 38 marga. Suku yang paling banyak ditemukan adalah Nephrolepidaceae dan terdiri atas Nephrolepis bisserata Sw. Schott, N. cordifolia (L.) C. Presl., N. exaltata (L.) Schott, N. falcata (Cav.) C. Chr., N. hirsutula (G. Forst.) C. Presl., dan N. radicans (Burm.) Kuhn. Selain itu, Psilotum nudum (L.) P. Beauv. merupakan jenis koleksi Pteridophyta yang masuk pada IUCN Red List dengan kategori terancam punah. Untuk mempermudah proses pemeliharaan dan monitoring, dilakukan penataan koleksi Pteridophyta dengan pengelompokan berdasarkan marga dan diurutkan mulai kelompok paling primitif hingga modern. Hasil penataan ini dapat memberikan nilai edukasi kepada pengunjung Kebun Raya Purwodadi, khususnya para pelajar.
Co-Authors Ahmad Syahrian Siregar, Ahmad Syahrian Dewi Ayu Lestari Elga Renjana Izmi Yulianah Kurniawan Budiarto Masnar, Adley L. Mas’udah, Shofiyatul Sasanti Widiarsih Yusuf, Hadi Mokhamad