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All Journal Jurnal Katalisator Jurnal Ilmiah Kesehatan Sandi Husada (JIKSH)
Besse Yuliana
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Agriculture, Biological Sciences & Forestry Chemistry Health Professions Medicine & Pharmacology Nursing Public Health

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OPTIMIZATION OF ANTIOXIDANT ACTIVITY OF 96% ETHANOL EXTRACT OF Chromolaena odorata L. ABTS RADICAL PROPHYLACTIC Usman, Yuri Pratiwi Utami; Rahmah Mustarin; Imrawati; Nurzadrina Wahyuddin; Suhaera; Besse Yuliana
JURNAL KATALISATOR Vol. 9 No. 1 (2024): Jurnal Katalisator Volume 9 No.1, April 2024
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v9i1.2817

Abstract

Choromolaena odorata L. is one of the plants used as an antioxidant. This research aims to determine the optimization of the antioxidant activity of 96% ethanol extract of C. odorata L. leaves using the ABTS method, a variety of drying techniques. Previous research has tested antioxidant activity using one drying technique, whereas this research uses a variety of drying techniques because the sample drying technique will affect its activity. Simplicia is obtained from various drying techniques. Then the simplicia was organoleptically tested and its water content was measured using the distillation method. The ethanol extract of Tacklean leaves was carried out using the maceration method using 96% ethanol solvent, then phytochemical screening was carried out using the TLC method and the antioxidant activity test of the ethanol extract of C. odorata L leaves was carried out using the ABTS method. The formation of the ABTS radical cation [2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) forms the basis of one of the spectrophotometric methods that has been applied to the measurement of total antioxidant activity of plants. The research results from the three drying techniques (50°C oven, direct sun drying, and air drying) showed organoleptic results in successive colors (brown, chocolate and dark green), while for shape and odor, namely dry leaves and a distinctive odor. The water content of simplicia ranges from 7.52-9.31%, soaked extract 8.4-10.3% and IC50 23.287-29.064 µg/mL and quarcetin as a comparison with IC50 2.292 µg/mL. Phytochemical screening results contain alkaloids, flavonoids, steroids and tannins. Oven drying at 50°C produced the greatest antioxidant activity with an IC50 value of 23.287 µg/mL which indicates very strong antioxidant power, although the antioxidant activity with a variety of drying techniques was all in the very strong category because it was <50 µg/mL.
Sexual behavior factors that influence the incidence of HIV/AIDS in men's groups’ male sex Efi Sarfiani; Dian Meiliani Yulis; Besse Yuliana
Jurnal Ilmiah Kesehatan Sandi Husada Vol. 13 No. 2 (2024): July - Desember
Publisher : LPPM Politeknik Sandi Karsa, South Sulawesi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35816/jiksh.v13i2.354

Abstract

Introduction: HIV/AIDS remains a significant public health problem in the world, including in Indonesia. The disease is spread through various pathways, but one of the groups most vulnerable to infection is Men Who Have Sex with Men (MSM). This study aims to analyze the influence of sexual behavior on the incidence of HIV/AIDS in the Men Having Sex with Men (MSM) group. Research Methodology: This type of research is quantitative research with a case-control research design. The population in this study consisted of cases and a control population. The population of cases in this study were all groups of Men Who Have Sex with Men (MSM) who had undergone an HIV test with positive (+) HIV test results and had taken ARV treatment at the Kendari City Regional Hospital for the last 6 (six) months and were domiciled as many as 349 people. Result: The results of the study showed that there was an influence of condom use (OR = 7.154), number of sexual partners (OR = 21,000), anal sex (OR = 7.042), and oral sex (OR = 3.367) on the incidence of HIV/AIDS in the Men Sex Men group. Conclusion: The conclusion is that the sexual behavior of using condoms, number of sexual partners, anal sex, and oral sex are risk factors for the occurrence of HIV/AIDS in the group of men having sex with men. Therefore, individuals in this group need to receive education about the risks, as well as effective prevention methods, such as consistent condom use, routine HIV testing, and open communication with sexual partners.
Co-Authors Efi Sarfiani Imrawati Nurzadrina Wahyuddin Rahmah Mustarin Suhaera Yulis, Dian Meiliani Yuri Pratiwi Utami