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All Journal Journal of Tropical Mycorrhiza Genbinesia Journal of Biology
Mohamad, Danial
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Immunology & microbiology

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Types of waste identification in Perintis Lake as an ecotourism conservation efforts Bone Bolango District, Gorontalo Province Mohamad, Danial; Lanti, Syahrul
Genbinesia Journal of Biology Vol. 4 No. 1 (2024): November 2024
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v4i1.51

Abstract

Perintis Lake is a lake area with potential for ecotourism. However, as activities progress, some areas have become littered with garbage. Therefore, the purpose of this study focuses on identifying the types of waste and determining the most common types of waste in the Perintis Lake area. This study uses a quantitative descriptive research method by identifying the types of waste scattered in the lake area using a sampling method categorized based on the size characteristics of mega-debris (>1 m), macro-debris (2.5 cm–1 m), meso-debris (5 mm–2.5 cm), and micro-debris (0.33 mm–5 mm). Based on the research activities, the sample collections locations are in two different transect areas, namely the Perintis Lake tourist area and the lake area directly facing residential areas. The total identification results obtained from both transects consist of 266 types of waste in five different categories: plastic waste, rubber, metal, glass, wood, and derivatives. Among the findings, plastic waste is the most dominant type of waste in the lake area, totalling 218 types. Furthermore, in terms of waste characterization based on size, macro-debris is the most abundant category in the lake area, particularly plastic waste, with a total of 168. The abundance of plastic waste in the research location is influenced by indiscriminate disposal by visitors to the environment and also by household activities directly facing the Perintis Lake area, resulting in an increasing spread of waste distribution over time.
Utilization sugarcane waste (Saccarhum officinarum L.) as a bioetanol basic material through a bioprocess engineering approach based microbes Mohamad, Danial; Lanti, Syahrul; Dwiyani, Annisa; Matalauni, Citra Leonita; Suduri, Adam
Genbinesia Journal of Biology Vol. 4 No. 1 (2024): November 2024
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v4i1.70

Abstract

Sugarcane (Saccharum officinarum L.) is a plant that is able to produce a lot of sugar content in its stem organs. The amount of sugar production from sugar cane, of course, produces and leaves bagasse waste. A number of studies have shown that bagasse waste still contains a lot of important materials, one of which contains lignocellulose substrates that have the potential to be converted into bioethanol raw materials. The purpose of this study is to determine, see and assess the production of bioethanol produced by utilizing sugarcane waste as raw material through a microbial-based bioprocess engineering approach. This research is an experimental type with the treatment of free cells and immobilized cells in bioethanol production by utilizing sugarcane waste as raw material with the stages of processing waste into flour, dried, hydrolyzed, and analyzed sugar content before ethanol production test. Based on the results of research on ethanol testing using free cells, the highest alcohol content was obtained at a concentration of 3% and 4%. For the test of ethanol content using immobilized cells, the highest ethanol content was obtained at a concentration of 4%.
ANTAGONISTIC ABILITY OF ENTOMOPATHOGENIC FUNGI METARHIZIUM ANISOPLIAE AND BEAUVERIA BASSIANA AGAINST MORTALITY OF WOOD GRASSHOPPER (VALANGA NIGRICORNIS) Mohamad, Danial; Lamangantjo, Chairunnisah
Journal of Tropical Mycorrhiza Vol. 4 No. 2 (2025): October
Publisher : Asosiasi Mikoriza Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/jtm.v4i2.87

Abstract

The entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana are potential biological control agents for suppressing the population of the wood grasshopper (Valanga nigricornis). This study aims to evaluate the antagonistic ability of the fungi M. anisopliae and B. bassiana against the wood grasshopper (Valanga nigricornis). This study is a descriptive study with a quantitative approach, applying inoculum suspension at four concentration levels (P1, P2, P3, and P4) and observing insect mortality and colony growth in culture media. The results showed that the P4 suspension treatment produced the highest mortality of 92% with a probit value of 1.28, while mortality in other treatments ranged from 0 to 75%. The highest infection viability showed that in the P4 treatment, the CFU value was 3.0×106 for M. anisopliae and 1.4×106 for B. bassiana. These findings indicate that high inoculum concentrations increase the antagonistic effectiveness and infection ability of entomopathogenic fungi.
Co-Authors Dwiyani, Annisa Lamangantjo, Chairunnisah Lanti, Syahrul Matalauni, Citra Leonita Suduri, Adam