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All Journal Biology, Medicine, & Natural Product Chemistry Genbinesia Journal of Biology
Matalauni, Citra Leonita
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Utilization sugarcane waste (Saccarhum officinarum L.) as a bioetanol basic material through a bioprocess engineering approach based microbes Mohamad, Danial; Lanti, Syahrul; Dwiyani, Annisa; Matalauni, Citra Leonita; Suduri, Adam
Genbinesia Journal of Biology Vol. 4 No. 1 (2024): November 2024
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v4i1.70

Abstract

Sugarcane (Saccharum officinarum L.) is a plant that is able to produce a lot of sugar content in its stem organs. The amount of sugar production from sugar cane, of course, produces and leaves bagasse waste. A number of studies have shown that bagasse waste still contains a lot of important materials, one of which contains lignocellulose substrates that have the potential to be converted into bioethanol raw materials. The purpose of this study is to determine, see and assess the production of bioethanol produced by utilizing sugarcane waste as raw material through a microbial-based bioprocess engineering approach. This research is an experimental type with the treatment of free cells and immobilized cells in bioethanol production by utilizing sugarcane waste as raw material with the stages of processing waste into flour, dried, hydrolyzed, and analyzed sugar content before ethanol production test. Based on the results of research on ethanol testing using free cells, the highest alcohol content was obtained at a concentration of 3% and 4%. For the test of ethanol content using immobilized cells, the highest ethanol content was obtained at a concentration of 4%.
Actinomycetes from Plant Rhizosphere in Gorontalo Karst Area as Plant Growth Promoting Rhizobacteria Matalauni, Citra Leonita; Retnowati, Yuliana; Katili, Abubakar Sidik; Kandowangko, Novri Youla; Hasan, Ani M.
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1047-1053

Abstract

This study aimed to describe Actinomycetes from the rhizosphere of plants in the Gorontalo karst area as Plant Growth Promoting Rhizobacteria (PGPR). The research method is based on a quantitative descriptive method. Soil samples from the rhizosphere were collected using a purposive sampling technique from 8 plant species at two locations in the Gorontalo karst region, namely the Tanjung Kramat Hills. The characteristics of PGPR in this study focused on phosphate solubilization ability, Indole Acetic Acid (IAA) production, antagonism against the fungus Fusarium oxysporum, and tolerance to fungicides. Potential PGPR isolates were analyzed for phylogenetic relationships based on 16S rRNA gene sequences. The results showed that there were 6 actinomycetes isolates (RzHKC-01, RzKO-02, RzO-03, RzAK-04, RzPK-05, and RzOC-06) from 8 plant species in the Gorontalo karst region. One actinomycetes isolate, RzPK-05, showed potential as a PGPR with the ability to solubilize phosphate and produce IAA at 0.165 mg/L and 0.133 mg/L, respectively. Isolate RzPK-05 exhibited antagonistic properties against Fusarium oxysporum with an inhibition rate of 82.24% and was tolerant to fungicides such as Captive, Benlate, and Thiramo up to a concentration of 100 mg/L. Molecular analysis based on the 16S rRNA gene and phylogenetic tree reconstruction indicated that the RzPK-05 isolate is closely related to the genus Streptomyces with 100% similarity.
Co-Authors Abubakar Sidik Katili Dwiyani, Annisa HASAN, ANI M. Lanti, Syahrul Mohamad, Danial Novri Youla Kandowangko Suduri, Adam Yuliana Retnowati