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All Journal BIOEDUKASI Jurnal Mitra Pengabdian Farmasi
Wd Windi Pratiwi
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Library & Information Science Public Health

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Pengenalan Penggunaan Spektrofotometer UV-Vis untuk Analisis Bahan Pewarna Tekstil Rhodamin B pada Makanan di Lingkungan Sekolah Anwar, Yelfi; Fathur Rahman; Ruminah; Sari, Sintya Puspita; Ance Tenouye; Akifah, Hana Tri; Ni’am, Laelatun; Riska rahmadani; Dhea Setyalova Wibawanto; Nurul Diniah; Lily Suciati; Agnes Lenoera; Eka Syafitri Puspita S; Ayunda Fajarni Rahmi; Wd Windi Pratiwi
Jurnal Mitra Pengabdian Farmasi Vol. 2 No. 1 (2022): Oktober 2022
Publisher : Akademi Farmasi YPPM Mandiri

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Abstract

The use of textile dyes in food has been a problem for a long time in Indonesia, one of which is textile dyes such as Rhodamine B or Methanol Yellow. The lack of knowledge of school children is a problem where repeated exposure to textile dyes contained in food will become a poison which, if not watched out for, will become a serious problem. Various diseases can arise with repeated exposure to toxic substances and can cause changes in the properties of the body's cells which can lead to malignancy and death. Even though there are synthetic dyes that are safe for food, people still use hazardous materials as food coloring for reasons that end up making big profits in food products that are made and sold, especially in the school environment. The content of Rhodamine B can be determined by UV-Vis Spectrophotometry. It was introduced to students so that school students know that there is a way to test for suspected use of dangerous dyes in the food being sold. The level of knowledge of school students was tested to compare the results before and after participating in counseling on the UV-Vis Spectrophotometer tool. The results of the Paired test show a significant number between the pre-test and post-test values, with a significance value (2-tailed) 0.000 <0.05 indicating a significant difference between the pre-test and post-test, which means there is a difference of Student knowledge which is very significant between before and after counseling about UV-Vis spectrophotometer analysis of Rhodamine B textile dyes in food.
Identification of Bioactive Compounds and Antibacterial Activity of Papaya Leaf Extract (Carica Papaya L.) as an Inhibitor of Pseudomonas Aeruginosa Wd Windi Pratiwi; Sogandi
BIOEDUKASI: Jurnal Biologi dan Pembelajarannya Vol. 23 No. 3 (2025): Vol. 23, No. 3, October 2025
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v23i3.53758

Abstract

This study explores the potential of papaya leaf extract (Carica papaya L.) as a natural antibacterial agent against Pseudomonas aeruginosa. The extraction process was carried out using 96% ethanol through maceration, followed by fractionation with n-hexane and ethyl acetate. Antibacterial activity was assessed using disc diffusion and liquid dilution methods to determine the inhibition zone and the Minimum Inhibitory Concentration (MIC).  Phytochemica creening confirmed the presence of alkaloids, flavonoids, saponins, and tannins in all tested fractions. Among them, the n-hexane fraction demonstrated the strongest antibacterial activity, with an inhibition zone of 15.10 mm at a 10% concentration and a MIC at 6%. Further nalysis using Gas Chromatography-Mass Spectrometry (GC-MS) identified 79 compounds in the n-hexane fraction, with several dominant compounds such as hexadecanoic acid methyl ester, pentadecanoic acid 14-methyl methyl ester, and phytol — all known for their antibacterial properties. These results suggest that the n-hexane fraction of papaya leaf extract holds promising potential for development as a natural antibacterial agent, particularly against Pseudomonas aeruginosa.
Co-Authors Agnes Lenoera Akifah, Hana Tri Ance Tenouye Anwar, Yelfi Ayunda Fajarni Rahmi Dhea Setyalova Wibawanto Eka Syafitri Puspita S Fathur Rahman Lily Suciati Ni’am, Laelatun Nurul Diniah Riska Rahmadani Ruminah Sari, Sintya Puspita Sogandi