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All Journal Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA
Kasip, L.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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White Blood Cell Differentiation Based on Toll-Like Receptor 4 (TLR4) Gene Polymorphism in Local Indonesian Chickens Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, L.; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6518

Abstract

Toll-like Receptors (TLRs) genes are a group of genes that transcribe TLRs proteins which act as receptors on the surface of phagocytic cells to recognize molecular patterns of pathogenic microbial components. This research aims to identify the variability of the TLR4 gene and its relationship with blood profiles in local Indonesian chickens. The samples used to identify TLR4 gene diversity in local Indonesian chickens were 135 samples. Research methods include DNA extraction, PCR amplification, RFLP analysis, and genotype determination. The genotyping results in the research were associated with a complete blood profile so that a genotype was obtained that could be used as a marker for resistance traits based on the blood profile. The results of this study showed that the TLR4 gene was polymorphic with three genotype types and an allele frequency of more than 0.01 in each KUB, Sensi, and Broiler CP 707 chicken. The frequency of the GG genotype is the highest compared to the AG and AA genotypes. White blood cell concentration values showed variations in the three genotypes of TLR4 gene and were still within normal standards, although statistically the differences were not significant.
Genetic Polymorphism of g.3768T>C loci of NRAMP1 gene in Kampung and KUB Chickens Maskur, Maskur; Muhsinin, Muhammad; Rozi, Tapaul; Jan, Rahma; Kasip, L.
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6682

Abstract

Natural Resistance-Associated Macrophage Protein 1 (NRAMP1) is a gene that regulates the body's resistance to illness. The genetic diversity of Kampung and KUB chicken population in Lombok island West Nusa Tenggara province was investigated based on g.3768T>C loci of NRAMP1 gene. Experimental animal consisted of 105 KUB and 67 Kampung chickens. The NRAMP1 gene polymorphisms in both population were identified using PCR-RFLP method. Genetic parameters of g.3768T>C loci were calculated using PopGene32. In this study, the locus g.3768T>C of the NRAMP1gene found three genotypes (TT, CT, CC) with the frequency of the C allele was higher than the T allele, respectively 0.709 and 0.210 in Kampung chicken, and 0.738 and 0.262 in KUB chicken. The mean value of observed heterozygosity (Ho) and expected heterozygosity (He) of g.3768T>C loci in both populations revealed low to medium genetic diversity, with the values 0.222 and 0.400, respectively. The mean value of observed heterozygosity is lower than expected, indicating that the population is heading towards a heterozygosity deficit and proving that the two populations are not in Hardy-Weinberg equilibrium. The mean PIC value of the NRAMP1 gene (g.3768T>C) in both chicken population was 0.319 and classified as fairly informative (0.50 > PIC > 0.25). Meanwhile, the value of the genetic differentiation index (Fst) for Kampung and KUB chicken population were 0.385 and 0.433, indicating that there is differentiation between two chicken population at the observed loci.
Pengembangan Usaha Pembibitan Ayam Kampung di Kabupaten Lombok Timur Maskur; Rozi, Tapaul; Jan, Rahma; Kasip, L.; Muhsinin, Muhammad
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.5576

Abstract

Ayam buras merupakan potensi di daerah yang tersebar diseluruh Indonesia, khususnya di daerah pedesaan. Jenis unggas ini mempunyai beberapa keunggulan dibandingkan dengan jenis unggas lainnya yaitu : mudah dipelihara, cepat beradaptasi dengan lingkungan dan umumnya tahan terhadap penyakit tertentu, peluang pasar masih terbuka lebar, dan dapat dilaksanakan dengan modal terbatas. Namun demikian, usaha ayam buras ini masih sulit ditingkatkan karena selain keterbatasan modal, ketersediaan bibit ayam buras masih sulit diperoleh secara berkelanjutan. Penyuluhan dan pelatihan ini dilaksanakan untuk meningkatkan keterampilan peternak agar dapat memproduksi bibit ayam yang berkualitas sehingga tersedia secara kontinyu dan mampu memilih induk yang baik untuk bibit. Pelatihan dilakukan di Desa denggen Kecamatan Selong Kabupaten Lombok Timur pada tanggal 22 Juli 2023. Hasil penyuluhan dan pelatihan menunjukkan bahwa peternak dapat memahami teknik menghasilkan bibit berkualitas yang ditunjukkan dengan keterampilan pada saat pelatihan. Peternak juga menunjukkan respon yang sangat baik dan sangat antusias mengikuti praktek serta banyaknya pertanyaan yang diajukan selama ceramah dan pelatihan.
Peningkatan Produksi Ayam Kampung Berbasis Pemuliaan Ternak di Kabupaten Lombok Timur Jan, Rahma; Maskur; Kasip, L.; Rozi, Tapaul; Muhsinin, Muhammad
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.5578

Abstract

Penyuluhan dan pelatihan ini bertujuan untuk meningkatkan keterampilan peternak agar dapat memproduksi bibit ayam yang berkualitas sehingga tersedia secara kontinyu dan mampu memilih induk yang baik untuk bibit. Pelatihan dilakukan di Desa denggen Kecamatan Selong Kabupaten Lombok Timur pada tanggal 22 Juli 2023. Hasil penyuluhan dan pelatihan menunjukkan bahwa peternak dapat memahami teknik menghasilkan bibit berkualitas yang ditunjukkan dengan keterampilan pada saat pelatihan. Peternak juga menunjukkan respon yang sangat baik dan sangat antusias mengikuti praktek serta banyaknya pertanyaan yang diajukan selama ceramah dan pelatihan. Peternak menyadari bahwa produktivitas ayam kampung secara garis besar dapat ditingkatkan dengan dua cara yaitu perbaikan mutu genetik bibit dan perbaikan pengelolaan.
Co-Authors Maskur Maskur Maskur Muhsinin, Muhammad Rahma Jan Rozi, Tapaul