Rahma Jan
Fakultas Peternakan Universitas Mataram Jl. Majapahit 62 Mataram. Telp.0370 633603. Fax.0370-640592. Pos 83125

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THE OTHER COLOUR PATERN OF BALI CATTLE IN WEST LOMBOK REGENCY Sudrana, I Putu; ., Lestari; Jan, Rahma; Rozy, Tapaul; Kasip, Lalu Moh.
Jurnal Lembaga Penelitian Universitas Mataram Vol 18, No 1 (2014): Jurnal Penelitian
Publisher : Jurnal Lembaga Penelitian Universitas Mataram

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Abstract

The objective of this study was to identify  other colour patern of Bali cattle and the proportion  of Bali cattle that have other colour  in region of West Lombok Regency. Survey method, observation, and photography were used in the study. The study was conducted at three districts sample that divided based on high, medium, and small population of Bali cattle and also based on the condition of transportation. This study  used 31 farmers as sample.  The results  showed   that there were 10 kinds of  single other colour patern of Bali cattle, but the number of Bali cattle that had other colour patern was 11.80%.
Correlation of Hatching Weight with Body Weight at Two Months of Age from Crossbreeding of Layer Chicken with Kampung Chicken Based on Four Comb Types Lestari Lestari; Muhammad Muhsinin; Erdi Khaidir; Rahma Jan
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 8 No 2 (2022): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v8i2.122

Abstract

Penelitian ini bertujuan untuk mengetahui korelasi bobot tetas dengan bobot badan umur dua bulan (bobot akhir) hasil persilangan ayam ras petelur dengan ayam kampung berdasarkan empat tipe jengger. Penelitian dilakukan di Dusun Dalem Lauq, Desa Sakra, Kecamatan Sakra, Kabupaten Lombok Timur, Nusa Tenggara Barat (NTB). Materi yang digunakan adalah 50 ekor F1 hasil persilangan induk ayam ras petelur Isa Brown dengan pejantan ayam kampung jengger tunggal (RT) 13 ekor, jengger mawar (RM) 11 ekor, jengger walnut (RW) 13 ekor dan jengger pea (RP) 13 ekor. Variabel yang diamati yaitu bobot tetas (BT), bobot akhir (BA), pertambahan bobot badan (PBB) dan korelasi BT dengan BA. Hasil analisis variansi BT, BA dan PBB di antara perlakuan tidak terdapat perbedaan yang nyata (P>0,05). Korelasi positif BT dengan BA pada RM (0,47) dan RT (0,06). Hasil analisis regresi BT berpengaruh positif terhadap BA pada F1 dari RM dan RT. Kata kunci: Korelasi, Persilangan, Ayam Ras Petelur, Ayam Kampung, Tipe Jengger
White Blood Cell Differentiation Based on Toll-Like Receptor 4 (TLR4) Gene Polymorphism in Local Indonesian Chickens Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, L.; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6518

Abstract

Toll-like Receptors (TLRs) genes are a group of genes that transcribe TLRs proteins which act as receptors on the surface of phagocytic cells to recognize molecular patterns of pathogenic microbial components. This research aims to identify the variability of the TLR4 gene and its relationship with blood profiles in local Indonesian chickens. The samples used to identify TLR4 gene diversity in local Indonesian chickens were 135 samples. Research methods include DNA extraction, PCR amplification, RFLP analysis, and genotype determination. The genotyping results in the research were associated with a complete blood profile so that a genotype was obtained that could be used as a marker for resistance traits based on the blood profile. The results of this study showed that the TLR4 gene was polymorphic with three genotype types and an allele frequency of more than 0.01 in each KUB, Sensi, and Broiler CP 707 chicken. The frequency of the GG genotype is the highest compared to the AG and AA genotypes. White blood cell concentration values showed variations in the three genotypes of TLR4 gene and were still within normal standards, although statistically the differences were not significant.
Genetic Polymorphism of g.3768T>C loci of NRAMP1 gene in Kampung and KUB Chickens Maskur, Maskur; Muhsinin, Muhammad; Rozi, Tapaul; Jan, Rahma; Kasip, L.
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6682

Abstract

Natural Resistance-Associated Macrophage Protein 1 (NRAMP1) is a gene that regulates the body's resistance to illness. The genetic diversity of Kampung and KUB chicken population in Lombok island West Nusa Tenggara province was investigated based on g.3768T>C loci of NRAMP1 gene. Experimental animal consisted of 105 KUB and 67 Kampung chickens. The NRAMP1 gene polymorphisms in both population were identified using PCR-RFLP method. Genetic parameters of g.3768T>C loci were calculated using PopGene32. In this study, the locus g.3768T>C of the NRAMP1gene found three genotypes (TT, CT, CC) with the frequency of the C allele was higher than the T allele, respectively 0.709 and 0.210 in Kampung chicken, and 0.738 and 0.262 in KUB chicken. The mean value of observed heterozygosity (Ho) and expected heterozygosity (He) of g.3768T>C loci in both populations revealed low to medium genetic diversity, with the values 0.222 and 0.400, respectively. The mean value of observed heterozygosity is lower than expected, indicating that the population is heading towards a heterozygosity deficit and proving that the two populations are not in Hardy-Weinberg equilibrium. The mean PIC value of the NRAMP1 gene (g.3768T>C) in both chicken population was 0.319 and classified as fairly informative (0.50 > PIC > 0.25). Meanwhile, the value of the genetic differentiation index (Fst) for Kampung and KUB chicken population were 0.385 and 0.433, indicating that there is differentiation between two chicken population at the observed loci.
Body Weight and Morphometric Characteristics of KUB Chicken at 60 Days of Age Al Farizi, Muhammad Salman; Jan, Rahma; Maskur, Maskur; Rozi, Tapaul; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7319

Abstract

Kampung Unggul Balitnak Chicken (KUB) is a highly favored Indonesian native chicken breed among breeders because of its rapid development rate and ability to thrive in many situations. This study aimed to investigate KUB chickens' growth performance and morphometric parameters at 60 days of age, a crucial phase for determining their suitability as commercial broilers. Using an intensive rearing system, 100 KUB chickens were reared for 60 days in group cages. Body weight measurements were recorded weekly, and morphometric traits were measured after the study period. The study's findings indicated that the mean body weight of KUB chickens at 60 days of age was 710.53±16.76 grams, suggesting a favorable growth rate. In addition, the morphometric analysis revealed favorable physical characteristics, including an average chest length of 100.55±3.70 mm, chest width of 47.47±3.12 mm, wing length of 165.93±4.34 mm, back length of 164.9±4.26 mm, back height of 205.51±3.99 mm, shank length of 63.23±3.69 mm, and shank circumference of 35.76±1.48 mm. The results indicate that KUB chickens exhibit desirable body weight and morphometric traits at 60 days old, proving their suitability in commercial broiler farming. To summarize, this study offers significant information regarding the growth performance and physical characteristics of KUB chickens during a critical phase of their development. The findings highlight the breed's capacity to serve as a practical choice for commercial broiler production, enhancing the variety and long-term viability of the poultry sector in Indonesia.
Association of Inducible Nitric Oxide Synthase (iNOS) Gene Diversity with Immunity Characteristics in Kampung Chicken Muhsinin, Muhammad; Jan, Rahma; Maskur, Maskur; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8296

Abstract

The immune system of Kampung chickens is vital for maintaining health and productivity, especially in combating bacterial infections. Among the genetic components involved, the inducible nitric oxide synthase (iNOS) gene is crucial for producing nitric oxide (NO), a molecule with strong antimicrobial properties. This study investigated the association between iNOS (AluI│g.15056T>C) gene polymorphism and immune traits in Kampung chickens to enhance disease resistance. Blood samples from 100 Kampung chickens were genotyped using PCR-RFLP, and immune parameters such as leukocyte count, macrophage activity, and bacterial resistance were evaluated. Statistical analysis revealed significant differences among TT, TC, and CC genotypes. The CC genotype exhibited superior performance, including the highest macrophage activity (91.74±1.92%), activated macrophage capacity (2279.49), and bacterial death rate (60.81±3.54%). These findings suggest that the CC genotype enhances NO production, strengthening the immune response to bacterial pathogens like Staphylococcus aureus and Salmonella Pullorum. Additionally, the polymorphism contributes to improved genetic diversity and immune efficiency in Kampung chickens. The study highlights the potential of incorporating the iNOS CC genotype in selective breeding programs to produce chickens with enhanced resilience. Future research should focus on interacting environmental factors with iNOS expression to optimize its application in chicken production.
Identification of Diversity and Genetic Distance of Indonesian Local Chicken Strains Based on Myostatin Gene Muhsinin, Muhammad; Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8297

Abstract

Indonesian local chickens are essential genetic resources with significant potential for advancing the national poultry industry due to their high genetic diversity, reflecting adaptation to tropical environments and traditional farming systems. This study analyzed the genetic diversity of Kampung, Sentul, KUB, SenSi, and Broiler chickens based on the MSTN g.4842T>G locus. Blood samples from 150 chickens underwent PCR-RFLP analysis using the BsrI restriction enzyme targeting exon 2 of the MSTN gene. Parameters such as observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), and Hardy-Weinberg equilibrium were assessed. Genetic distances were calculated using PHYLIP, and a dendrogram was constructed with UPGMA. The study identified two alleles (G and T) and three genotypes (GG, GT, TT), with the T allele being dominant, particularly in Broiler chickens (78.3%). Ho was lower than He in all populations, indicating a heterozygosity deficit. PIC values ranged from 0.282 (Broiler) to 0.367 (SenSi), classifying the MSTN locus as moderately polymorphic. Kampung and KUB chickens showed the closest genetic relationship, while Broiler chickens were the most distinct. These findings underscore the potential of local chickens in breeding programs for adaptability and productivity. Further research with additional markers is recommended to enhance sustainable poultry farming.
Identification of Single Nucleotide Polymorphism (SNP) in Exon 5 of the Prolactin Gene in Native Chickens Rozi, Tapaul; Maskur, Maskur; Jan, Rahma; Kasip, Lalu Muhammad; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8342

Abstract

The prolactin gene (PRL) in chickens is one of the genes that regulates broodiness and egg production in laying hens. Single Nucleotide Polymorphism (SNP) is a form of single nucleotide variation in the DNA base sequence at specific locations in the genome that can influence differences in quantitative traits in livestock. This study aimed to identify SNPs in the prolactin gene of native chickens. The research methods included DNA extraction, PCR amplification, and DNA sequence analysis for genotype determination of individuals. The results revealed four mutation points at positions g.8052 bp T>C, g.7886 T>A, g.7823 A>G, and g.8069 T>C. All mutation points in exon 5 were substitution mutations. Statistical analysis showed that all loci were polymorphic and the population was in Hardy–Weinberg equilibrium.