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g.640T>C Polymorphism of the TGF-β2 Gene is Associated with Salmonella pullorum Resistance in Indonesian Chickens Muhsinin, Muhammad; Ulupi, Niken; Gunawan, Asep; Wibawan, I Wayan Teguh; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1109.044 KB) | DOI: 10.20884/1.jap.2017.19.2.597

Abstract

The objectives of this study were to identify polymorphism of transforming growth factor β2 (TGF-β2) gene associated with Salmonella pullorum resistance in Indonesian chickens. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were used to genotype Indonesian chickens. Analysis of polymorphism was conducted by using PopGen 3.2 software. The effect of genotype on S. pullorum resistance was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 278 chickens from 7 Indonesian chicken populations (Sentul, Merawang, Pelung, Kampung, Parent Cobb broiler, The F1 crossbreed of Kampung x Parent Cobb broiler (KB) and F2 KB x KB. The product of amplification was 284 bp. The TGF-β2| RsaI locus was polymorphic in all populations, producing two alleles (T and C) and three genotypes (TT, CT, and CC). The result from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the C allele in all populations. The χ2 analysis showed that the 6 chicken populations were deviated from Hardy-Weinberg equilibrium, with the exception of the Parent Cobb broiler chicken. The association result showed that TT genotype was significantly associated with S. pullorum resistance in Sentul chicken. Although the leukocyte concentration, leukocyte differentiation and H/L ratio in Sentul chicken with three of TGF-β2 genotypes (TT, TC, and CC) were not statistically different. In conclusion, polymorphism in the TGF-β2 chicken gene can be used as a candidate marker to increase S. pullorum immune response.
Preliminary Study on Moisture, Fat, and Protein Contents of Bee Bread From Apis cerana from Different Regions in North Lombok Regency, Indonesia Erwan Erwan; Lara Dwi Franti; Dwi Kusuma Purnamasari; Muhammad Muhsinin; Agussalim Agussalim
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.5

Abstract

Apis cerana is one of the honeybee species from the Apis genus that produce honey, bee bread, royal jelly, and propolis. The objective of this study was to determine the honeybee forages as the pollen source and to evaluate the moisture, fat, and protein contents of bee bread from the bee of Apis cerana from different regions (Sigar Penjalin, Teniga, and Medana villages) in North Lombok, Indonesia. The methods used to determine the pollen source were taken flowers sample and then checking pollen at anther of flowers. The moisture, fat, and protein contents were determined using proximate analysis. The results showed that the honeybee forages as the pollen source from Sigar Penjalin village were maize, cashew, damar, kapok, calabash, breadfruit, passion fruit, and acacia. The plant flowers as the pollen source from Teniga village were coconut, coffee, cacao, citrus, guava, leucaena, papaya, and sugar palm, while those from Medana village were jackfruit, mangosteen, Java apple, maize, rice, candlenut, soybean, and banana. The moisture and fat contents of bee bread from Sigar Penjalin and Teniga villages were similar, and both were lower than the moisture and fat contents of bee bread from Medana village. The protein content of bee bread from Sigar Penjalin and Medana villages were similar, and both were lower than the protein content of bee bread from Teniga village. Thus, the bee bread from Teniga village was higher in protein content for all beekeeping regions, however Teniga village and Sigar Penjalin were similar in moisture and fat contents.
The Honey Quality of Apis mellifera with Extrafloral Nectar in Lombok West Nusa Tenggara Indonesia Erwan Erwan; Muhammad Harun; Muhammad Muhsinin
Journal of Science and Science Education Vol. 1 No. 1 (2020): October
Publisher : Pascasarjana, Mataram University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.735 KB) | DOI: 10.29303/jossed.v1i1.482

Abstract

The purpose of the present study was to analyze Apis mellifera honey's quality given extrafloral nectar feed from Arenga pinnata sap and Cocus nucifera sap as stimulatory nutrition. The chemical compositions of honey, such as reducing sugar content, sucrose content, the acidity of honey, moisture content, and diastase enzyme activity, were measured. The comparison between A. mellifera honey's chemical compositions on Arenga pinnata sap and Cocus nucifera sap ware was analyzed using the student t-test (GraphPad Instant Statistical Program). The result from the analysis of reducing sugar content showed that the A. mellifera honey from Cocus nucifera sap (73.69± 0.21%) had a higher (P<0.05) than the Arenga pinnata sap (60.15±2.13%). The significant differences (P<0.05) in the acidity of A. mellifera honey from Arenga pinnata sap (43.00±7.48) compared with Cocus nucifera sap (22.00±2.14). The sucrose content, moisture content, and diastase enzyme activity were not significant differences between the A. mellifera honey from Arenga pinnata sap compared with the A. mellifera honey from Cocus nucifera sap. In conclusion, the chemical compositions of A. mellifera honey with extrafloral nectar (Arenga pinnata sap and Cocus nucifera sap) are good quality and indicate that the honey falls under the limits of international standards. The A. mellifera honey from Cocus nucifera sap has a higher sugar reduction content and lower acidity than the A. mellifera honey from Arenga pinnata sap
Identifikasi Jenis Tanaman Pakan Lebah Madu sebagai Sumber Nektar dan Polen Erwan Erwan; Dwi Kusuma Purnamasari; Ria Resti; Muhammad Muhsinin
JURNAL TRITON Vol 13 No 2 (2022): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v13i2.254

Abstract

Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis tanaman yang berpotensi sebagai pakan lebah dan tanaman penghasil nektar dan polen sebagai sumber pakan lebah. Lokasi penelitian dilaksanakan di peternakan lebah madu di Kecamatan Kayangan Kabupaten Lombok Utara. Metode penelitian menggunakan survei inventarisasi jenis-jenis tanaman pakan lebah madu penghasil nektar dan polen. Metode pengambilan data meliputi pembuatan petak contoh, inventarisasi tanaman didalam petak contoh, kesaksian kunjungan lebah pada tanaman, studi pustaka dan pembuatan kalender pembungaan. Hasil penelitian menunjukkan bahwa secara keseluruhan terdapat 61 jenis tanaman sumber pakan lebah di Desa Salut Kecamatan Kayangan Kabupaten Lombok Utara. Terdapat 25 jenis tanaman penghasil nektar, 10 jenis tanaman penghasil polen dan 26 jenis tanaman penghasil polen dan nektar. Frekuensi kunjungan lebah terbanyak ditemukan pada tanaman kelapa, palm, durian, jambu mete, kopi, kakao, blimbing, klengkeng, rambutan, pepaya, kaliandra, bunga matahari, kecubung, bunga pukul delapan, bayam, bunga lantana, bunga zenia, putri malu, kemangi, sawi. Tingginya frekuensi kunjungan menunjukkan tingkat kesukaan lebah terhadap tanaman tersebut. Potensi tanaman yang dapat berbunga sepanjang tahun adalah kelapa, kopi, kakao, kersen, dan putri malu. Pakan lebah madu di Desa Salut Kecamatan Kayangan Kabupaten Lombok Utara akan selalu tersedia disebabkan berbedanya masa berbunga dari setiap tanaman. Kecukupan kebutuhan pakan lebah dapat terpenuhi dari tanaman lainnya saat beberapa tanaman tidak berbunga. Hasil penelitian ini diharapkan ada upaya tindak lanjut dari pemerintah dan kelompok ternak lebah madu untuk pelestarian jenis tanaman pakan lebah madu agar tetap tersedia sepanjang tahun.
Study of Morphological and Morphometric Characteristics of Worker Bees Tetragonula clypearis in North Lombok Regency, West Nusa Tenggara Province, Indonesia Erwan Erwan; Munaris Munaris; Muhammad Muhsinin
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.4132

Abstract

This study aimed to describe the morphometric characteristics of the worker bee Tetragonula clypearis in Salut Village, District Kayangan, North Lombok Regency. The research material used was worker bee Tetragonula clypearis taken from 40 stup in two livestock groups, namely 20 stup in the Harapan Keluarga group and 20 stup in the Tunas Muda group in Salut Village, Kayangan District, North Lombok Regency. Each stup took three samples of worker bees for morphometric analysis (n=120). Morphological characters were observed under a stereo microscope connected to an Optilab camera. The character of T. clypearis is the thorax is black and hairy, and there are six hair bands on the mesoscutum and dark brown tibia. Metasoma has a variety of colours, ranging from pale yellow with black stripes, yellow and black, and pale yellow. The morphometric measurements included body length, head width, wing length, genna width, second flagellomere diameter, and body weight. The results showed that the seven morphometric measurement variables of the worker bee Tetragonula clypearis did not show significant differences (P>0.05) in the two cultivation groups. The value of the diversity coefficient <15% indicates that the morphometrics of the worker bee Tetragonula clypearis is uniform in both cultivation groups in Salut Village, District, Kayangan, North Lombok Regency.
Manajemen Perkawinan Ternak Sapi Di Kecamatan Pujut Lombok Tengah Untuk Mendukung Program Desa Seribu Sapi Maskur; Rahma Jan; Lestari; Tapaul Rozi; Muhammad Muhsinin
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 1 (2023): Januari - Maret
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i1.3344

Abstract

Sapi Bali merupakan ternak lokal asli Indonesia sangat berpotensi untuk dikembangbiakkan dan sebagai plasma nutfah harus tetap dilestarikan. performans sapi Bali cenderung menurun disebabkan aplikasi program pemuliaan yang kurang ketat, sehingga mutu genetiknya rendah. Dalam upaya memperbaiki mutu genetik, Pola Peternakan Inti Terbuka (Open Nucleus Breeding) tampaknya sesuai untuk diterapkan dalam pengembangan sapi Bali yang banyak melibatkan peternakan rakyat sebagai populasi dasar dengan dukungan kegiatan seleksi pada berbagai lapisan peternakan dan harus ditunjang dengan manajeman perkawinan yang baik dan benar. Teknik manajemen perkawinan sapi potong dapat dilakukan dengan menggunakan (1) Intensifikasi kawin alam (IKA) dengan pejantan terpilih, (2) teknik inseminasi buatan (IB) dengan semen beku (frozen semen) dan teknik IB dengan semen cair (chilled semen). Pengaturan manajemen perkawinan dan teknik pemberian pakan saat beranak, kawin, sapih dan bunting tua pada sapi potong induk diharapkan mengikuti suatu model yang dikenal sebagai/dengan kalender perkawinan dan program pemberian pakan (surge feeding) pre dan pasca beranak. Tujuan kalender ini untuk mempercepat birahi kembali setelah beranak untuk segera dikawinkan dan memudahkan terjadi fertilisasi (kebuntingan) berikutnya.
OPTIMASI POPULASI SAPI BALI BETINA UNGGUL MELALUI INSEMINASI BUATAN DAN SOSIALISASI DI KOPERASI PETERNEKAN 'TUNAS RIDHO ILAHI' DI PULAU LOMBOK, NTB DENGAN STRAW SEXING Lalu Unsunnidhal; Asmarani Kusumawati; Muhammad Muhsinin; Raudatul Jannah
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 7, No 4 (2023): December
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v7i4.19332

Abstract

ABSTRAKPenelitian ini bertujuan untuk meningkatkan populasi dan kualitas sapi Bali betina unggul di Koperasi Peternakan "Tunas Ridho Illahi," Kabupaten Lombok Timur, NTB. Koperasi ini menjadi wadah bagi peternak sapi potong dengan fokus pada budidaya Sapi Bali, yang memiliki makna kultural dan genetik di Pulau Lombok. Meskipun diakui sebagai Plasma Nuftah Indonesia, produktivitas sapi Bali belum optimal disebabkan oleh kualitas bibit yang buruk. Solusi diusulkan melalui sosialisasi dan pelaksanaan inseminasi buatan dengan bibit sapi Bali betina unggul, dengan kolaborasi antara dosen Universitas Mataram dan koperasi. Metode melibatkan koordinasi awal, sosialisasi, dan pelaksanaan inseminasi buatan. Koordinasi awal membangun pemahaman tentang masalah dan rencana kerja. Sosialisasi mengedukasi tentang pemilihan sapi betina unggul dan inseminasi buatan. Langkah ini diharapkan meningkatkan produktivitas dan kualitas ternak. Hasilnya menunjukkan kolaborasi sukses dalam mengatasi kendala, memulai perbaikan kualitas genetik sapi Bali betina unggul. Melalui diskusi, presentasi, dan partisipasi peternak, sosialisasi efektif menginformasikan dan memotivasi. Kegiatan ini menciptakan kesadaran dan keterlibatan aktif komunitas peternak. Pelatihan praktis memberi panduan tentang persiapan sapi betina untuk inseminasi buatan. Meskipun tahap inseminasi belum terlaksana, pendekatan kolaboratif ini diharapkan memberikan dampak positif dalam mengembangkan sektor peternakan di wilayah ini. Kata kunci: sapi bali betina unggul; inseminasi buatan; sosialisasi; kolaborasi; produktivitas ternak ABSTRACTThis study aims to increase the population and quality of superior female Bali cattle in the Animal Husbandry Cooperative "Tunas Ridho Illahi," East Lombok Regency, NTB. This cooperative is a forum for beef cattle breeders with a focus on the cultivation of Bali cattle, which has cultural and genetic significance on the island of Lombok. Even though it is recognized as Indonesian Nuftah Plasma, the productivity of Bali cattle is not optimal due to the poor quality of the breed. The solution was proposed through socialization and implementation of artificial insemination with superior female Bali cattle, in collaboration between Mataram University lecturers and cooperatives. The method involves initial coordination, socialization, and implementation of artificial insemination. Initial coordination builds understanding of the problem and work plan. Socialization educates about the selection of superior female cows and artificial insemination. This step is expected to increase the productivity and quality of livestock. The results show a successful collaboration in overcoming obstacles, starting to improve the genetic quality of superior female Bali cattle. Through discussions, presentations, and farmer participation, socialization effectively informs and motivates. This activity creates awareness and active involvement of the farming community. Practical training provides guidance on preparing cows for artificial insemination. Although the insemination stage has not yet been carried out, this collaborative approach is expected to have a positive impact on developing the livestock sector in this region. Keywords: superior female bali cattle; artificial insemination; collaboration; livestock productivity
Semen Evaluation, Preparation of Bangkok Roosters, and Insemination to Indonesian Native Hens Asnawi; Maskur; Adji Santoso Dradjat; Muhammad Muhsinin
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5779

Abstract

The success of artificial insemination in chickens is very dependent on sperm quality. Sperm temperature and diluent during storage can affect sperm quality. This study evaluated the quality of Bangkok chicken sperm diluted with 5% glucose and NaCl stored at 5 ⁰C and 26 ⁰C. The research design used was completely randomized. The data obtained were analyzed using a variance. The results showed that the motility of sperm preserved for 9 hours at 26 ⁰C with infused NaCl and Glucose 5% differed significantly (P< 0.05) with motility of 50±0.0% and 34±8.94%, respectively. While preservation under 5 ⁰C for 9 hours with NaCl, better than Glucose 5% (P<0.05) with motility of 58.00±10.95% and 38.00±10.95%, respectively. The viability of sperm preserved at 26 ⁰C with glucose 5% extender was better than NaCl (P<0.05) with a value of 58.93±1.27% and 33.43±1.27%, respectively. While preservation at 5 ⁰C of viabilities of sperm under Glucose 10% and NaCl were not significantly different (P>0.05) from values of 52.57±5.15% and 48.14±8.09%, respectively. The abnormality of sperm stored at 26 ⁰C and 5 ⁰C for 9 hours with NaCl and Glucose 5% were not different (P>0.05). Insemination using 100 million sperm with infused NaCl extender from 18 eggs produced 94.44% (n=17) fertility and 72.22% (n=13) hatchability. While insemination using infuse glucose 5% extender, 11 eggs produced 63.64% (n=7) fertility and 54.54% (n=6) hatchability. The infusing NaCl extender produced better sperm quality, fertility, and hatchability than glucose 5% at 5 ⁰C.
Correlation of Hatching Weight with Body Weight at Two Months of Age from Crossbreeding of Layer Chicken with Kampung Chicken Based on Four Comb Types Lestari Lestari; Muhammad Muhsinin; Erdi Khaidir; Rahma Jan
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 8 No 2 (2022): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v8i2.122

Abstract

Penelitian ini bertujuan untuk mengetahui korelasi bobot tetas dengan bobot badan umur dua bulan (bobot akhir) hasil persilangan ayam ras petelur dengan ayam kampung berdasarkan empat tipe jengger. Penelitian dilakukan di Dusun Dalem Lauq, Desa Sakra, Kecamatan Sakra, Kabupaten Lombok Timur, Nusa Tenggara Barat (NTB). Materi yang digunakan adalah 50 ekor F1 hasil persilangan induk ayam ras petelur Isa Brown dengan pejantan ayam kampung jengger tunggal (RT) 13 ekor, jengger mawar (RM) 11 ekor, jengger walnut (RW) 13 ekor dan jengger pea (RP) 13 ekor. Variabel yang diamati yaitu bobot tetas (BT), bobot akhir (BA), pertambahan bobot badan (PBB) dan korelasi BT dengan BA. Hasil analisis variansi BT, BA dan PBB di antara perlakuan tidak terdapat perbedaan yang nyata (P>0,05). Korelasi positif BT dengan BA pada RM (0,47) dan RT (0,06). Hasil analisis regresi BT berpengaruh positif terhadap BA pada F1 dari RM dan RT. Kata kunci: Korelasi, Persilangan, Ayam Ras Petelur, Ayam Kampung, Tipe Jengger
Budidaya Lebah Tetragonula sp Sebagai Upaya Pencegahan Stunting dan Peningkatan Kesejahteraan Masyarakat Desa Bayan, Lombok Utara Abdullah, Mohammad Ibnu Hajar; Yulia Azmi Khotimah; Muhammad Muhsinin; Erwan; Ryan Aryadin Putra; Agussalim
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 1 (2025): Januari-Maret 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i1.10643

Abstract

Kabupaten Lombok Utara angka stunting mencapai 29,3%. kondisi ini disebabkan oleh kekurangan gizi, asupan gizi yang berkualitas rendah, dan keterbatasan ekonomi. Memanfaatkan madu Tetragonula sp, yang terkenal kaya akan vitamin, mineral, dan asam amino serta memiliki khasiat untuk meningkatkan kekebalan tubuh, adalah salah satu solusi yang mungkin dilakukan. Manfaat dari budidaya lebah Tetragonula sp adalah kebutuhan lahan yang rendah, biaya perawatan yang rendah, dan ramah lingkungan. Pada bulan Mei - Juli 2023, sebuah program percontohan dilakukan di Dusun Montong Baru untuk meningkatkan pengetahuan dan keterampilan masyarakat dalam budidaya lebah Tetragonula sp. berdasarkan aktivitas budidaya “good practices”. Program ini mencakup sosialisasi, pelatihan, bantuan teknis, dan penyediaan koloni lebah tambahan. Sejumlah 36 peserta dari kelompok “Bunga Mekar”, kader Posyandu dan masyarakat setempat terlibat dalam kegiatan ini. Hasil dan capaian setelah sosialisasi dan pelatihan masyarakat dapat meningkatkan pemahaman terkait manfaat produk lebah Tetragonula sp, khususnya madu. Selain itu jumlah koloni lebah meningkat menjadi 65 koloni, dan produksi madu per panen menjadi 6 – 9 liter. Pendampingan intensif selama tiga bulan juga dilakukan untuk memastikan penerapan teknik budidaya yang optimal dan produksi produk yang berkualitas tinggi. Selain meningkatkan status gizi keluarga, madu Tetragonula sp. juga memberikan dampak ekonomi dengan menciptakan peluang usaha lokal. Pelaksanaan program ini diharapkan dapat diterapkan di daerah lain dengan angka stunting yang tinggi, sehingga memberikan dampak luas bagi peningkatan kesejahteraan dan pemenuhan gizi masyarakat.