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All Journal SCIENCE TECH: Jurnal Ilmiah Ilmu Pengetahuan dan Teknologi Jurnal Biologi Tropis
Khafida, Wilda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Engineering Immunology & microbiology Materials Science & Nanotechnology

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Effectiveness of Length and Weight Growth of Tenebrio molitor L. Larvae with Plastic Waste Feeding Hashifah, Fathimah Nurfithri; Muslihah, Niken Istikhari; Khafida, Wilda; Haryanto, Trisno; Ambarningrum, Trisnowati Budi
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7665

Abstract

Inorganic waste accumulating in plastic waste, including styrofoam, which has accumulated in large quantities in waste disposal sites (TPS), is challenging to decompose into the environment. Black beetles (Tenebrio molitor) are insects that decompose inorganic waste and can decompose polystyrene waste. The presence of bacteria in the digestion of insects contributes to the decomposition of plastic waste in the form of styrofoam. This study aimed to determine how effective the larvae of T. molitor are in degrading polystyrene waste (styrofoam) through larval development from the process of nine instar stages. In order to develop more prospective larvae, the research was conducted in multiple stages, including initial maintenance and treatment as well as data gathering to test the growth of larval length. The results are known from the length of the larval body, and it is hoped that the larvae can later be used to help decompose small to large-scale styrofoam waste discharged into the environment. Larvae of T. molitor can eat inorganic materials like styrofoam and organic ones like bran. The ability to do this is demonstrated by the growth of the larvae's weight and length, with the rise in larval length in each treatment having the most beneficial result.
Soil Bacteria Community Composition Indicating Soil Quality in Different Tillage Practices Khafida, Wilda; Ratnaningsih, Hanim Rahayuani
Science Tech: Jurnal Ilmu Pengetahuan dan Teknologi Vol 10 No 2 (2024): Agustus
Publisher : Universitas Sarjanawiyata Tamansiswa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30738/st.vol10.no2.a17546

Abstract

Organic tillage practice of current study used fully cattle manure, while semi-organic tillage used cattle manure and 10% of synthetic fertilizers as transition to reduce chemical inputs in order to improve sustainable production and ecosystem health. Despite the important role of soil microbes in soil of agriculture, we still have limited understanding of the complex response of microbial composition to organic and semi-organic tillage practices. The aim of this work was to evaluate the impact of use of organic and semi-organic tillage practices on bacteria abundance, richness and functional diversity of microbial communities. Analysis of soil bacteria using nutrient agar by serial dilutions of soil suspension was performed to quantify bacteria abundance. They were further identified and categorized into IAA-producing bacteria and non IAA-producing bacteria groups in Tryptic Soy Agar (TSA). Bacteria abundance was significantly higher in semi-organic tillage than organic tillage. Even though bacteria richness did not show statistically significant, beneficial effect of organic tillage was reflected in higher bacteria richness in term of isolates. The assessment of their functional diversity on producing IAA hormone demonstrated higher IAA-producing bacteria than non IAA-producing bacteria in both tillage practices, indicating the availability of rich substrate through introduction of cattle manure improve soil quality and beneficial for soil bacteria. We observed that among all physicochemical properties, C-organic indicated highly significant, higher in organic tillage than semi-organic tillage. It has been proposed that the availability of organic carbon may favour bacteria as resource of energy.   Keywords: Bacteria community composition; IAA-producing bacteria; Organic tillage; Semi-organic tillage
Co-Authors Haryanto, Trisno Hashifah, Fathimah Nurfithri Muslihah, Niken Istikhari Ratnaningsih, Hanim Rahayuani Trisnowati Budi Ambarningrum