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All Journal Jurnal Biologi Tropis Molekul: Jurnal Ilmiah Kimia
Muslihah, Niken Istikhari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Materials Science & Nanotechnology

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Immobilization of Urease from Psophocarpus tetragonolobus L. DC. using Natrium Alginate Supporting Matrix Zusfahair, Zusfahair; Ningsih, Dian Riana; Lestari, Puji; Bilalodin, Bilalodin; Aryanti, Eva; Muslihah, Niken Istikhari
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.7335

Abstract

Urease is an enzyme that has the role to hydrolyzes urea into ammonia and carbon dioxide. Immobilization is one of the most efficient strategies to improve its activity recovery and properties of urease. This research started with the germination of winged beans for 8 days. The winged bean was extracted by grinding using a mortar and pestle and then added with phosphate buffer at pH 7. The solution was homogenized using a stirrer and then centrifuged in cold conditions so that an extract of urease was obtained. Urease extracts were immobilized using a chitosan-supporting matrix. Optimization of the immobilization process of urease extract includes the concentration of chitosan and sodium tripolyphosphate (TPP) and contact time. The obtained was free and immobilized urease activities then tested using the Nessler method and measured using a UV-Vis spectrophotometer with a wavelength of 500 nm. The obtained data were then statistically tested using ANOVA. Urease-chitosan beads were further tested in repeated use and analyzed with SEM-EDX (Scanning Electron Microscopy-Energy Dispersive X-ray). The results showed that the optimum conditions for making urease-chitosan beads were a concentration of 4% (w/v), 2.5% (w/v) TPP, and 60 minutes of contact time, resulting in an activity value of 15.076 U/mL, which can be used 5 times with 46% activity from the initial activity. The EDX analysis results after the addition of the enzyme showed atom composition changes leading to increasing carbon and nitrogen contents. The existence of phosphor showed that TPP was a chitosan cross-link compound. Keywords: Chitosan, immobilization, TPP, urease, winged bean
Optimization and Characterization of Urease Immobilization from Red Lentil Seeds (Lens culinaris) Using Chitosan zusfahair, zusfahair; Ningsih, Dian Riana; Bilalodin, Bilalodin; Fatoni, Amin; Luthfia, Adilla; Purwati, Purwati; Muslihah, Niken Istikhari; Apriliadina, Inessa Putri
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13134

Abstract

ABSTRACT. Urease is an enzyme that plays a vital role in catalyzing the hydrolysis of urea into ammonia (NH3) and carbon dioxide (CO2). This study focuses on the isolation of urease from red lentil seeds, followed by its immobilization. The objective of this research is to optimize and characterize urease that has been immobilized using chitosan and activated with glutaraldehyde. Red lentil seeds were processed with a mortar and pestle at low temperatures (4 °C) to obtain a crude enzyme extract, which was then concentrated using 50% acetone (P50) prior to immobilization. The optimization process for P50 urease immobilization involved assessing various factors, including chitosan concentration, glutaraldehyde concentration, temperature, and the immersion duration in glutaraldehyde. The findings revealed that the optimal conditions for immobilizing P50 urease were achieved at a chitosan concentration of 0.75%, with a 2% glutaraldehyde soak at 25 °C for 2 hours, resulting in an enzyme activity of 7.042 U/g. The immobilized P50 urease demonstrated the ability to be reused up to 7 times while maintaining 51% of its initial activity. Scanning Electron Microscopy (SEM) analysis indicated morphological changes in the beads after the addition of glutaraldehyde and the enzyme, shifting from a rounded to an irregular shape. Additionally, Fourier Transform Infrared Spectroscopy (FTIR) analysis identified C-N and C=N peaks, confirming the successful incorporation of glutaraldehyde. Keywords: immobilization, red lentil seeds, glutaraldehyde, chitosan, urease
Effectiveness of Length and Weight Growth of Tenebrio molitor L. Larvae with Plastic Waste Feeding Hashifah, Fathimah Nurfithri; Muslihah, Niken Istikhari; Khafida, Wilda; Haryanto, Trisno; Ambarningrum, Trisnowati Budi
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7665

Abstract

Inorganic waste accumulating in plastic waste, including styrofoam, which has accumulated in large quantities in waste disposal sites (TPS), is challenging to decompose into the environment. Black beetles (Tenebrio molitor) are insects that decompose inorganic waste and can decompose polystyrene waste. The presence of bacteria in the digestion of insects contributes to the decomposition of plastic waste in the form of styrofoam. This study aimed to determine how effective the larvae of T. molitor are in degrading polystyrene waste (styrofoam) through larval development from the process of nine instar stages. In order to develop more prospective larvae, the research was conducted in multiple stages, including initial maintenance and treatment as well as data gathering to test the growth of larval length. The results are known from the length of the larval body, and it is hoped that the larvae can later be used to help decompose small to large-scale styrofoam waste discharged into the environment. Larvae of T. molitor can eat inorganic materials like styrofoam and organic ones like bran. The ability to do this is demonstrated by the growth of the larvae's weight and length, with the rise in larval length in each treatment having the most beneficial result.
Co-Authors AMIN FATONI Apriliadina, Inessa Putri Aryanti, Eva Bilalodin Bilalodin Dian Riana Ningsih Haryanto, Trisno Hashifah, Fathimah Nurfithri Khafida, Wilda Luthfia, Adilla PUJI LESTARI Purwati Purwati Trisnowati Budi Ambarningrum Zusfahair Zusfahair