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All Journal BIOEDUKASI Life Science and Biotechnology Berita Biologi
Nasution, Sakinah Salman Ahmad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Library & Information Science

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Bioactivity of Endophytic Fungi Fusarium brachygibbosum Isolated from the Stem of Avicennia marina as a Candidate for New Drug Source Oktiansyah, Rian; Noviyanto, Noviyanto; Iskandar, Damayanti; Nasution, Sakinah Salman Ahmad; Laksono, Pandu Jati; Kurniawati, Alfia Rahma; Pancasari, Adelia Rizki; Aputri, Farah Nuriessa; Yogyaswari, Sekar Ayoe
BIOEDUKASI: Jurnal Biologi dan Pembelajarannya Vol. 23 No. 3 (2025): Vol. 23, No. 3, October 2025
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v23i3.53787

Abstract

Indonesia's biodiversity holds significant potential as a source of medicinal compounds, one of which is Avicennia marina, known for its antioxidant and antibacterial properties. However, due to its protected conservation status, its utilization is limited. An alternative approach to harness its benefits is through symbiotic microorganisms such as endophytic fungi. This study aimed to isolate and evaluate the bioactivity of the endophytic fungus Fusarium brachygibbosum isolated from the stem of A. marina as a potential source of novel therapeutic agents. The methodology involved fungal isolation, morphological identification, and evaluation of antibacterial activity using the disc diffusion method and antioxidant activity using the DPPH assay. The results demonstrated that the obtained isolate (coded ADM2) exhibited strong antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Bacillus subtilis. Moreover, the antioxidant assay of the ADM2 isolate revealed a very strong potential (IC50 < 20 µg/mL), indicating its potent ability to scavenge free radicals. Based on these findings and the literature on F. brachygibbosum, this species shows great potential as a source of bioactive compounds with medical applications, particularly in treating infections and preventing degenerative diseases. Further in vivo studies are necessary to confirm the therapeutic potential of this fungus as a safe and effective drug candidate.
Bioactivity of Endophytic Fungal Extract Isolated from Purun (Eleocharis dulcis (Burm.f.) Trin. ex Hensch) Oktiansyah, Rian; Pratiwi, Mardiyah; Kharisma, Sully Pudja; Fauzan, Ahmad Rizki; Utami, Riza; Amelia, Dea; Noviyanto, Noviyanto; Nasution, Sakinah Salman Ahmad
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.52579

Abstract

The study investigates the bioactivity of endophytic fungi isolated from Eleocharis dulcis (Burm.f.) Trin. ex Hensch., focusing on their potential as alternative sources of bioactive compounds. E. dulcis, a plant commonly found in wetland ecosystems, has traditionally been used for its medicinal properties. However, the massive extraction of the plant may lead to ecological imbalance, prompting the exploration of endophytic fungi as a sustainable alternative. The objective of this research is to isolate, identify, and evaluate the antimicrobial and antioxidant properties of endophytic fungi from Eleocharis dulcis. Endophytic fungi were isolated aseptically, followed by morphological identification. Antioxidant and antimicrobial assays were conducted to assess the bioactivity of the fungal extracts. Antioxidant activity was evaluated using the DPPH method, while antibacterial activity was assessed using the disc paper diffusion method. The results revealed that several isolates (from leaves: LP1 – LP6, from root: RP1 – RP3) exhibited significant antimicrobial activity against Escherichia coli and Staphylococcus aureus, with Penicillium spp. and Cladosporium spp. showing the highest levels of bioactivity. Additionally, all fungal isolates demonstrated very strong antioxidant activity, with most achieving an IC50 below 20 µg/mL. In conclusion, the findings suggest that endophytic fungi from E. dulcis represent a valuable source of bioactive compounds with potential applications in pharmaceuticals. Further research is required to isolate and characterize the active compounds, as well as to evaluate their efficacy through in vivo studies
Antibacterial and antioxidant activity of Endophytic Fungal Extract Isolated from the Root of Sonneratia caseolaris Noviyanto, Noviyanto; Oktiansyah, Rian; Nasution, Sakinah Salman Ahmad; Utami, Riza; Amelia, Dea
Life Science and Biotechnology Vol. 3 No. 1 (2025): May 2025
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

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Abstract

Sonneratia caseolaris is a mangrove species of considerable pharmacological importance; however, its natural populations are increasingly threatened by habitat degradation and overexploitation. Endophytic biotechnology provides a sustainable alternative for exploring the bioactive potential of this plant without imposing additional pressure on its fragile ecosystem. This study aimed to evaluate the antibacterial and antioxidant activities of endophytic fungal extracts isolated from the roots of S. caseolaris. Morphological and molecular techniques were employed for fungal identification, while antibacterial activity was assessed using the disc diffusion assay, and antioxidant capacity was evaluated through the DPPH radical scavenging assay. The selected fungal isolate, RSC2, exhibited strong antibacterial and antioxidant activities. Thin Layer Chromatography (TLC) analysis confirmed the presence of flavonoid compounds, and molecular identification verified the isolate as Aspergillus niger. This study reports, for the first time, the isolation of A. niger from S. caseolaris roots with the ability to produce flavonoid metabolites exhibiting notable bioactive potential. These findings provide a scientific basis for future in vivo investigations aimed at developing natural compounds from mangrove-associated endophytes as promising pharmaceutical candidates.
BIOACTIVITY OF ENDOPHYTIC FUNGI EXTRACTS RESIDING IN THE ROOTS OF MISTLETOE (Dendrophthoe pentandra) Oktiansyah, Rian; Habisukan, Ummi Hiras; Noviyanto, Noviyanto; Nasution, Sakinah Salman Ahmad; Febiantika, Imaniar; Octavia, Risky
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

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Abstract

Mistletoe (Dendrophthoe pentandra) is a parasitic plant that has traditional medicinal properties. This study examines the endophytic fungi found in mistletoe roots and their bioactivity. Endophytic fungal species are identified using their morphological traits. Potato Dextrose Broth (PDB) medium is utilized for both culture and extraction, with ethyl acetate as a solvent. The antioxidant test employed the DPPH method, whereas the antibacterial test used the paper disc diffusion method. Mistletoe roots yielded four endophytic fungus isolates (BR1–BR4). The findings of morphological study revealed that BR1 (Pythium sp.), BR2 (Trichoderma sp.), BR3 (Fusarium sp.), and BR4 (Mortierella sp.). The endophytic fungus BR4 (Mortierella sp.) had the highest potential antioxidant and antibacterial activities. This endophytic fungal extract has the potential to be a source of novel medications through additional investigation by isolating the pure component and in vivo studies.
Co-Authors Aputri, Farah Nuriessa Damayanti Iskandar Dea Amelia, Dea Fauzan, Ahmad Rizki Febiantika, Imaniar Kharisma, Sully Pudja Kurniawati, Alfia Rahma Noviyanto Noviyanto, Noviyanto Octavia, Risky Oktiansyah, Rian Pancasari, Adelia Rizki Pandu Jati Laksono Pratiwi, Mardiyah Riza Utami, Riza Ummi Hiras Habisukan, Ummi Hiras Yogyaswari, Sekar Ayoe