Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Indonesian Journal of Pharmaceutical Science and Technology
Taskworo, Dodik
Unknown Affiliation
Computer Science & IT Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Exosome Hypoxic-MSCs, Glutathione, and Vitamin C: Effect on IL-10 Levels and CD-163 Expression Utami, Wulan Dyah; Muhar, Adi Muradi; Sumarawati, Titiek; Putra, Agung; Setiawan, Eko; Ibrahim, Sugeng; Taskworo, Dodik; Haitamy, Mohammad Nurrizki
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 3 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i3.60941

Abstract

Hyperpigmentation of the skin is a result of ultraviolet B (UVB) exposure, which causes oxidative stress due to increased reactive oxygen species (ROS), leading to various skin problems, including melanin accumulation. Exosomes can affect melanocyte activity. Exosomes, as small vesicles released by cells, can affect melanocyte activity and play an important role in various hyperpigmentation processes. The study aims to determine the effect of exosome mesenchymal stem cell hypoxia (EH-MSC) and glutathione with vitamin C on IL-10 levels and CD163 expression. IL-10 gene expression was measured using qRT-PCR, while CD163 expression was analyzed via immunohistochemical staining. A total of 30 male C57BL/6 mice were used and randomly assigned to five different treatment groups. The highest expression of IL-10 was observed in the EH-MSCs-treated group (K4), although the difference was not statistically significant compared to the control (p = 0.135). In contrast, the group receiving a combination of EH-MSCs with glutathione and vitamin C (K5) exhibited the highest percentage of CD163 expression, with a statistically significant difference (p = 0.00). These findings demonstrate that the administration of EH-MSC and glutathione with vitamin C significantly increased the expression of CD163, but insignificantly increased IL-10 in C57BL/6 mice with a UVB-induced hyperpigmentation model.
Hypoxic MSCs Reduce UV-B Collagen Loss by Modulating CD68 and TNF-α Expression Ati, Sri Umi; Putra, Agung; Sumarawati, Titiek; Setiawan, Eko; Ibrahim, Sugeng; Taskworo, Dodik
Indonesian Journal of Pharmaceutical Science and Technology Vol 13, No 1 (2026)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v13i1.63650

Abstract

Ultraviolet-B (UV-B) radiation-induced skin photoaging is marked by collagen degradation and chronic inflammation, with limited effective treatments currently available. Hypoxic-preconditioned mesenchymal stem cells (H-MSCs) offer a novel therapeutic approach due to their immunomodulatory capabilities. This study evaluated the effects of H-MSCs at two doses (2.5×105 and 5×105 cells) on UVB-induced collagen loss, focusing on CD68 expression and TNF-α levels in a rat model. Male Wistar rats were divided into five groups: healthy controls, UV-B-exposed negative controls (saline), positive controls (hyaluronic acid), and two H-MSC-treated groups. After UV-B exposure (160 mJ/cm², five times per week for two weeks), validated H-MSCs were administered subcutaneously. Collagen content was assessed histologically, while CD68 gene expression and TNF-α levels were measured by qRT-PCR and ELISA, respectively. UVB exposure led to significant reductions in collagen and increased levels of inflammatory markers. H-MSC treatment showed dose-dependent anti-inflammatory effects, with the higher dose (5×105 cells) optimally reducing CD68 expression and TNF-α levels, nearly matching healthy controls. These results suggest that H-MSCs, particularly at higher doses, may be a promising therapy for UVB-induced skin damage and collagen loss.
Co-Authors Agung Putra Ati, Sri Umi Eko Setiawan Eko Setiawan Haitamy, Mohammad Nurrizki Ibrahim, Sugeng Muhar, Adi Muradi Titiek Sumarawati Utami, Wulan Dyah