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All Journal Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Media Penelitian dan Pengembangan Kesehatan
Sanita, Hanifah Velda
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Environmental Science Medicine & Pharmacology

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Edukasi Pemanfaatan Aloe vera Sebagai Bahan Alternatif Untuk Mengurangi Kandidiasis Qurrohman, Muhammad Taufiq; Sitohang, Dahlan; Rizki, Andita Ainal; Shaleha, Annisa Azizah; Rasid, Bryan Aditya Nur; Sanita, Hanifah Velda; Annisa, Mariana Suryanti Nur; Maharani, Raras; Tambunan, Yohana Nathalia
Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Vol 9, No 4 (2025): Jurnal Pelayanan dan Pengabdian Masyarakat (PAMAS)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM Universitas Respati Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/pamas.v9i4.3668

Abstract

Candidiasis is an opportunistic fungal infection that frequently affects the skin, particularly among individuals with suboptimal personal hygiene and those living in humid environments. One natural substance that has the potential to inhibit the growth of Candida albicans is Aloe vera, which contains saponin and lupeol with antifungal activity through the mechanism of disrupting fungal cell membrane integrity. Based on this background, a community service program was conducted to enhance the knowledge and skills of residents in utilizing Aloe vera as a natural ingredient for the prevention of candidiasis. The activity took place in Bentakan Village, Baki District, Sukoharjo Regency, involving 18 women of productive age as participants. The method included a pre-test, interactive lecture, demonstration of Aloe vera soap production, post-test, and evaluation. The average pre-test score of 54.44 increased to 77.77 in the post-test, with the Wilcoxon test showing p < 0.05. This finding indicates a significant improvement in participants’ understanding of candidiasis prevention and promotes positive changes in personal hygiene behavior. The sustainability of this program is expected to be supported through follow-up training and the establishment of community empowerment groups as small-scale producers of herbal soap with economic value.
Detection of the Murine Double Minute2 (MDM2) gen in formalin-fixed tissue samples using PCR Sanita, Hanifah Velda; Sayekti, Fitria Diniah Janah
Media Penelitian dan Pengembangan Kesehatan Vol. 36 No. 1 (2026): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v36i1.3605

Abstract

Background: Tissue preservation is a crucial procedure in pathological studies to maintain cellular and structural integrity, commonly performed through formalin fixation. However, formalin fixation may cause DNA degradation, posing challenges for molecular analysis. Detection of cancer biomarker genes, such as Murine Double Minute 2 (MDM2), can be performed using Polymerase Chain Reaction (PCR), a technique for amplifying specific DNA fragments. Objective: This study aimed to determine the presence of the MDM2 gene in formalin-preserved tissues stored for different periods using PCR. Methods: A descriptive qualitative design with random sampling was employed. The study was conducted at the Anatomical Pathology and Molecular Biology Laboratories of Sekolah Tinggi Ilmu Kesehatan Nasional. Samples included 10 hair and nail tissues as controls, and formalin-fixed tissues stored for 2 days, 1 month, 2 months, and 8 years. DNA isolation was performed using the Geneaid gSYNC™ DNA Extraction Kit. DNA quality was assessed by 1.5% agarose gel electrophoresis, while concentration and purity were measured using a UV-Vis spectrophotometer. DNA concentrations ranged from 320–420 ng/µL, with purity ratios between 0.8 and 1.125. Results: The results showed that the MDM2 gene (178 bp amplicon) was detectable in tissues stored for up to 2 months but was not detected in tissues stored for 8 years. Conclusion: These findings indicate that storage duration affects the success of gene detection in formalin-fixed tissues.
Co-Authors Annisa, Mariana Suryanti Nur Maharani, Raras Muhammad Taufiq Qurrohman, Muhammad Taufiq Rasid, Bryan Aditya Nur Rizki, Andita Ainal Sayekti, Fitria Diniah Janah Shaleha, Annisa Azizah Sitohang, Dahlan Tambunan, Yohana Nathalia